排序方式: 共有4条查询结果,搜索用时 0 毫秒
1
1.
本文应用化学降解法测定了adr亚型乙型肝炎病毒pADR-1 DNA中包含表面抗原基因(s基因)的XhaI BamHI片段顺序,共1279碱基对。比较了adr亚型与已知的adw,adyw,ayw的s基因的核苷酸顺序及其编码的HBsAg氨基酸顺序的差异,发现了一些新的变异位点,差异集中分布在HBsAg的亲水区域。但在一些可能具有生物功能的位点,各亚型间并无变异,在遗传和进化中是相对保守的。比较s基因区和非s基因区的核苷酸变异情况表明,非s基因区的变异频率高一倍,s基因区是相对保守的。 相似文献
2.
THE COMPLETE NUCLEOTIDE SEQUENCE OF THE CLONED DNA OF HEPATITIS B VIRUS SUBTYPE adr IN pADR-1 总被引:4,自引:0,他引:4
The complete nucleotide sequence of the cloned hepatitis B virus DNA subtype adr in pADR-1 wasdetermined by Maxam and Gilbert's method. It is 3215 base pairs in size, which is 27 bp longer thanthe sequence of the adr pHBr330, as reported by Ono et al. The nucleotide difference between pADR-1and adr pHBr330 is about 2% while those between pADR-1 and adw as well as ayw are 9.3% and 9.7%respectively. In this paper, the heterogeneity and homogeneity of the S gene, the C gene and the othercoding regions in pADR-1 and in the other subtypes are compared and discussed. 相似文献
3.
4.
The nucleotide sequence of the XhoI-BamHI fragment (1279bp), which contains the sur-face antigen gene (S gene) of HBVadr, was determined by Maxam and Gilbert's method.By comparing the differences both of the nuclectide sequence in the S gene and its codedamino acid sequence between adr and those reported for adw, ayw and adyw, some new var-iation sites were discovered. The differences were mainly distributed in the two hydrophilieregions. However, at those sites which might show biological function, there were no varia-tions among different subtypes, they are relatively conservative in heredity and evolution.Comparing the variation of the nucleotide sequence in the S gene region with that in thenon-S gene region, it is shown that the frequency of variation in the non-S gene regiondoubled that in the S gene region. Tim S gene region is more conservative. 相似文献
1