Tantalum Nitride Nanosheets for Photoacoustic Imaging-Guided Photothermal Cancer Therapy

In this study, the synthesis of TaN nanosheets and their application in theranostic agents is reported. After coating polyethylene glycol (PEG) on the TaN nanosheets, the as-synthesized PEG-modified TaN nanosheets (TaN-PEG) show good stability and biocompatibility. Because of their high absorbance in the near-IR region, TaN-PEG can be utilized as photoacoustic imaging contrast agents for tumor imaging. Moreover, TaN-PEG has significant photothermal conversion performance, exhibiting effective laser-induced tumor ablation capability. The TaN-PEG possessing excellent photoacoustic contrast effect and photothermal properties thus have great promise in theranostic applications, especially imaging-guided cancer treatment.     

3.0T MRI检查结合X线钼靶诊断乳腺恶性肿瘤的研究

本研究探讨3.0T磁共振成像(MRI)结合X线钼靶诊断乳腺恶性肿瘤的价值。采用回顾性研究方法,选取乳腺肿块患者110例162个病灶,给予3.0T MRI及X线钼靶检查。经病理确诊为恶性病变101个;恶性病灶形态不规则、边缘毛刺、时间-信号强度曲线(TIC)类型Ⅲ型和早期增强率≥60%比例明显高于良性病灶(P<0.05),而分叶状比例和表观扩散系数(ADC)值明显低于良性病变(P<0.05);恶性病变X线钼靶表现:形态不规则、钙化、结构不对称和大导管征比例明显高于良性病变(P<0.05);MRI联合X线钼靶诊断乳腺恶性病变的灵敏性、准确性和阴性预测值明显高于MRI诊断(P<0.05)。3.0T MRI检查结合X线钼靶诊断乳腺恶性肿瘤有较好的价值。     

Versatile Near-Infrared Super-Resolution Imaging of Amyloid Fibrils with the Fluorogenic Probe CRANAD-2

CRANAD-2 is a fluorogenic curcumin derivative used for near-infrared detection and imaging in vivo of amyloid aggregates, which are involved in neurodegenerative diseases. We explore the performance of CRANAD-2 in two super-resolution imaging techniques, namely stimulated emission depletion (STED) and single-molecule localization microscopy (SMLM), with markedly different fluorophore requirements. By conveniently adapting the concentration of CRANAD-2, which transiently binds to amyloid fibrils, we show that it performs well in both techniques, achieving a resolution in the range of 45–55 nm. Correlation of SMLM with atomic force microscopy (AFM) validates the resolution of fine features in the reconstructed super-resolved image. The good performance and versatility of CRANAD-2 provides a powerful tool for near-infrared nanoscopic imaging of amyloids in vitro and in vivo.     

FRET-based ratiometric fluorescent detection of arginine in mitochondrion with a hybrid nanoprobe

A ratiometric fluorescent hybrid nanoprobe CDs-1 for arginine(Arg),exhibiting high sensitivity(the limit of detection,LOD,being 6.5×10^-8 mol/L) and excellent selectivity and anti-interference ability,was fabricated through fluorescence resonance energy transfer(FRET) and the electrostatic attraction between positively-charged hemicyanine molecules and negatively-charged carbon dots(CDs).Arg can be quantitatively detected in the concentration range from 6.0×10^-5 mol/L to 2.7×10^-4 mol/L.Further,due to its ability to target mitochondrion and low cytotoxicity,intracellular Arg was succes s fully tracked through ratiometric fluorescence imaging.     

A Far-Red Emitting Fluorescent Chemogenetic Reporter for In Vivo Molecular Imaging

Far-red emitting fluorescent labels are highly desirable for spectral multiplexing and deep tissue imaging. Here, we describe the generation of frFAST (far-red Fluorescence Activating and absorption Shifting Tag), a 14-kDa monomeric protein that forms a bright far-red fluorescent assembly with (4-hydroxy-3-methoxy-phenyl)allylidene rhodanine (HPAR-3OM). As HPAR-3OM is essentially non-fluorescent in solution and in cells, frFAST can be imaged with high contrast in presence of free HPAR-3OM, which allowed the rapid and efficient imaging of frFAST fusions in live cells, zebrafish embryo/larvae, and chicken embryos. Beyond enabling the genetic encoding of far-red fluorescence, frFAST allowed the design of a far-red chemogenetic reporter of protein–protein interactions, demonstrating its great potential for the design of innovative far-red emitting biosensors.     

Noninvasive Temperature Measurement in Dental Materials Using Nd3+, Yb3+ Doped Nanoparticles Emitting in the Near Infrared Region

In this work, the application of near infrared (NIR)-emitting NaYbF₄:1%Tm³⁺@NaLuF₄:30%Nd³⁺ core–shell nanoparticles is reported for noninvasive probing and monitoring the temperature during photopolymerization of dental materials. When excited at 808 nm, the synthesized nanoparticles emit NIR photoluminescence (PL) with two distinctive peaks at 865 and 980 nm which correspond to radiative transitions from the doped Nd³⁺ and Yb³⁺ ions, respectively. Luminescence intensity ratio between these two bands is found to vary with temperature due to temperature-dependent electronic excitation energy transfer between Nd³⁺ and Yb³⁺ ions at the core/shell interface. This finding allows luminescence ratiometric evaluation of the in situ temperature during photopolymerization of resin cement (doped with nanoparticles) in a veneer placement procedure. In addition, the NIR emission also enables PL imaging of the distribution of the adhesive under the veneer. The results highlight that rare-earth ions–doped nanoparticles with both excitation and emission in the NIR spectral range are advantageous for both PL-based nanothermometry and imaging due to the reduced attenuation of NIR light by dental ceramics.     

曲面相机阵列多分辨成像方法

针对现有成像系统因数据冗余而无法兼顾大视场、高分辨、高效性的问题,结合人眼视网膜变分辨成像和并列式复眼成像原理,设计一种多分辨率成像的复合仿生成像系统.该成像系统按照球面和平面兼顾的曲面布局方式,利用11个相机镜头构建相机阵列,组成了四个等级分辨率的子眼拍摄模块.通过物距100 m的远景实验和物距10 m的近景实验发现,该系统在实现高分辨成像的同时,获得总视场达150.8°×37.8°.多分辨率成像实验结果表明,该系统获取的图像的分辨率从中心视场到边缘视场逐渐降低,并且相较于中心清晰全视场成像,四级分辨率成像的拼接图像数据量减少了17.2倍的数据冗余.     

空中目标多波段红外辐射特性描述与仿真分析

针对空中目标在复杂背景下的探测需求,根据实际目标的运动特性,分析目标在飞行高度、飞行姿态角改变时的辐射特点,基于MODTRAN计算得到大气辐射和衰减数据,建立目标的三维模型、热辐射和反射模型,搭建空中目标的红外成像仿真系统.分析和仿真结果表明:在中波波段,目标尾焰的红外辐射比蒙皮强很多,在长波波段,蒙皮的红外辐射比较强,仿真图像的细节比较多,尾焰的红外辐射虽然有所减弱,红外成像效果依旧很好;相同探测条件下,由于位置越高大气越稀薄,探测器的可探测距离会变得比较远.目标红外辐射特性的分析和红外仿真系统的搭建对缩短红外探测器的研制周期和进一步确定探测器波段和系统分辨率等指标提供了参考依据.     

OCS经由F 31Π里德堡态生成CO(X1Σ+)+S(1D2)产物的波长相关性光解离

本文利用时间切片离子速度成像技术在134∽140 nm波段研究了OCS分子经由F 3¹Π里德堡态的真空紫外光解离动力学. 在选取的5个分别对应OCS(F 3¹Π, v₁=0∽4)的伸缩振动激发的光解波长，实验测得了来自CO(X¹Σ⁺)+S(¹D₂)产物通道的SS(¹D₂))实验影像，并获得了总平动能谱和CO(X¹Σ⁺, v)共生产物的振动布居及角分布. 结果分析表明OCS分子解离生成CO(X¹Σ⁺)+S(¹D₂)产物的过程经历了上态F 3¹Π 与C_?v和C_s构型的下电子态间非绝热耦合过程. 实验结果显示了很强的波长相关性：OCS (F 3¹Π, v₁)的较低转动激发态(v₁=0∽2)和较高转动激发态(v₁=3, 4)的CO(X¹Σ⁺)产物的振动布居和角分布具有显著差异，表明该解离过程中具有不同的解离机理. 本结果提供了振动耦合可能对真空紫外光解离动力学产生关键作用的相关证据.