Analysis of peptides and protein digests by reversed phase high performance liquid chromatography–electrospray ionisation mass spectrometry using neutral pH elution conditions

In this study, the advantages of carrying out the analysis of peptides and tryptic digests of proteins under gradient elution conditions at pH 6.5 by reversed-phase liquid chromatography (RP-HPLC) and in-line electrospray ionisation mass spectrometry (ESI-MS) are documented. For these RP separations, a double endcapped, bidentate anchored n-octadecyl wide pore silica adsorbent was employed in a capillary column format. Compared to the corresponding analysis of the same peptides and protein tryptic digests using low pH elution conditions for their RP-HPLC separation, this alternative approach provides improved selectivity and more efficient separation of these analytes, thus allowing a more sensitive identification of proteins at different abundance levels, i.e. more tryptic peptides from the same protein could be confidently identified, enabling higher sequence coverage of the protein to be obtained. This approach was further evaluated with very complex tryptic digests derived from a human plasma protein sample using an online two-dimensional (2D) strong cation-exchange (SCX)-RP-HPLC-ESI-MS/MS system. Again, at pH 6.5, with mobile phases of different compositions, improved chromatographic selectivities were obtained, concomitant with more sensitive on-line electrospray ionisation tandem mass spectrometric (ESI-MS/MS) analysis. As a consequence, more plasma proteins could be confidently identified, highlighting the potential of these RP-HPLC methods with elution at pH 6.5 to extend further the scope of proteomic investigations.     

Convective Instability of the Flow of a Binary Mixture under Conditions of Vibration and Thermal Diffusion

Stability of a plane-parallel flow of a nonuniformly heated binary mixture filling a vertical layer located in a field of gravity and in a high-frequency vibrational field is studied. The axis of vibrations is directed along the layer. The case of rigid and isothermal boundaries of the layer impermeable for the mixture is considered. The influence of thermal diffusion on the evolution of the admixture and the thresholds of flow stability is taken into account. The study is performed on the basis of equations for averaged fields. An asymptotic method with the use of the perturbation wavenumber as a small parameter is applied in the long-wave limit. For arbitrary values of the wavenumber, the limit of stability was determined by numerical integration. Charts of stability of gaseous and liquid binary mixtures are plotted. __________ Translated from Prikladnaya Mekhanika i Tekhnicheskaya Fizika, Vol. 47, No. 2, pp. 77–84, March–April, 2006.     

Plasma in a high-frequency electric field with a reflective condition on the boundary

An analytical solution is obtained for the linearized problem of the behavior of a collisional gas plasma in a half-space in an external alternating electric field. It is assumed that the electrons are mirror-reflected from the plasma boundary. The solution is used for finding the screened field. The case of an external field frequency close to the plasma frequency is investigated separately.     

GaN HEMT器件结构的研究进展

Ga N高电子迁移率晶体管(HEMT)具有大的禁带宽度、高电子饱和速度、异质结界面的高二维电子气浓度、高击穿电压以及高的热导率,这一系列特性使它在高频、高功率、高温等领域得到了广泛的认可。本文首先论述了制约氮化镓高电子迁移率晶体管器件性能提高所遇到的问题及解决方法;然后,着重从优化材料结构设计和器件结构设计的角度,阐述了氮化镓高电子迁移率晶体管器件在高频高功率领域的最新研究进展;最后,讨论了器件进一步发展的方向。     

低温等离子体物理进展

概述了低温等离子体物理研究现状、发展及其应用     

多体动力学程序切断铰的处理方法

多体系统动力学从非树系统派生出树系统的计算需要进行切断铰的处理。切断铰约束方程的形成是进行多体系统程序编写时的重要部分,其处理过程复杂,需要一定的技巧。本文引入了约束正交补轴的概念,详细介绍了几种典型(旋转铰、万向节、棱柱铰、旋转棱柱组合铰)切断铰位移约束方程、速度约束方程、加速度约束方程的形成方法,并给出了详细的程序化过程,该方法适用于任何类型的切断铰。最后给出相应算例,结果表明本文的方法能快速、正确地形成切断铰约束方程。     

基于驻留时间补偿的抛光误差控制方法

 针对计算机控制光学表面成形中光学表面存在中高频误差的问题,提出了一种基于驻留时间补偿的有效控制方法。分析了抛光误差的形成机理和影响因素,对系统的误差影响因素进行分类和定量描述,构建了抛光过程中磨损影响因子、浓度变化影响因子和系统影响因子。基于各影响因素的影响因子对抛光驻留时间的求解函数进行了修正,提出采用离散最小二乘法对修正的函数求解驻留时间。研究表明：这种补偿方法能提高计算机控制光学表面成形技术中加工模型的精度,减小光学表面的残余误差。     

质子交换LiNbO3波导调制器多模截止波长的测试技术研究

提出并实验了一种采用倒置输入的结构来测定Y分支LiNbO3波导相位调制器的多模截止波长的新方法,模式耦合分析表明,倒置输入的Y分支波导的多模传输的输出功率具有随波长变动发生振荡的特征,该特征可用于定量判定多模截止波长.方法的有效性得到了波束传播法(BPM)仿真和样品实验两方面的验证.     

高频引力波与现代宇宙学

文章简要综述了由典型现代宇宙学模型和膜振荡模型预期的微波频带的高频引力波的特性和可能的观测方案，以及研究这一课题的重要科学意义和所面临的挑战和机遇．     

Analysis for Transmission Characteristics of Closed Trapezoidal-Groove Waveguide with Boundary Element Method

In this paper, the cut-off and dispersion characteristics of a new kind of closed trapezoidal-groove waveguide that has more advantages than open groove waveguides are analyzed with the help of the boundary element method, the relationship between cut-off wavelengths and variations of guide's relative sizes is discussed in detail. The calculated results are in good agreement with those in the relevant reference. So, the obtained conclusions are very important in theoretical studies and actual applications of closed trapezoidal-groove waveguides for millimeter and submillimeter waves.