Real-Time Observation of Pyoverdine Dissolving Ferric Hydroxide

Pyoverdine is one of the siderphores excreted by Pseudomonas aeruginosa that can help microbe to uptake iron in vitro. To determine the effect of pyoverdine chelating with iron, we purified the free pyoverdine and applied the dynamic laser light scattering (DLS) to detect the interaction between the pyoverdine and ferric hydroxide. The real-time DLS data analysis indicated that pyoverdine can directly combine with Fe(OH)₃ to form complexes and these substances are gradually degraded by themselves then completely disappeared. In our experiment, we have demonstrated that pyoverdine may not only chelate ferric ion but also availably dissolve ferric hydroxide which assists bacteria to survive in iron-deficient environments.     

实时观测绿脓杆菌素溶解氢氧化铁

绿脓杆菌素是绿脓杆菌分泌的铁载体,它可以帮助细菌在体外有效摄取铁.为了观测绿脓杆菌素与铁的结合作用,提纯了游离态的绿脓杆菌素,并采用动态激光光散射来观察绿脓杆菌素和氢氧化铁的相互作用.实时观测光散射数据分析表明绿脓杆菌素可以直接和Fe（OH）₃作用形成复合物,这种复合物会自身逐渐降解直到完全消失.实验证明绿脓杆菌素不仅能够和铁离子相结合,还可以通过溶解氢氧化铁来帮助细菌在缺铁环境中生存.