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排序方式: 共有18条查询结果,搜索用时 15 毫秒
1.
为研究液态锂在电磁驱动限制器表面的铺展特性,设计了与EAST限制器接口相同的限制器测试平台。该平台运行时真空环境可达10-4Pa,对被测限制器可加热至350℃。在限制器锂回路管道上,利用外部2T水平磁场以及竖直方向施加的最大为200A的直流电流,形成电磁驱动力驱动下的锂液循环回路。测试平台设有顶部和正面两个观察窗,能够在可视化条件下,完成液态锂限制器的铺展性测试实验。基于该测试平台,对首次设计的双通路液态锂限制器,研究了不同实验温度和驱动电流下液态锂在限制器表面的流动铺展情况。结果发现,锂的流量和限制器表面结构是影响锂液铺展的主要因素。  相似文献   
2.
3-cube-based Förster resonance energy transfer (FRET) microscopy, a sensitized acceptor FRET quantification method, has been widely used to visualize dynamic protein–protein interaction in living cells. Determining the FRET sensitized-quenching transition factor (G factor) of a particular donor-acceptor pair and optical system is crucial for 3-cube FRET quantification. We here improved the acceptor photobleaching-based G factor determination method (termed as mPb-G) and the two-plasmid-based G factor determination method (termed as mTP-G) for rapid and reliable measurement of the G factor. mTP-G method determines G factor by simultaneously detecting three images of cells exclusively expressing each of two tandem constructs with multiple donors and multiple acceptors. This method circumvents switchover of the cells exclusively expressing each of the two constructs. mPb-G method images G factor by detecting three images of cells expressing a donor-acceptor tandem FRET construct before and after partially photobleaching acceptor. We performed the two methods on our dual-channel wide-field FRET microscope to obtain reliable G factor, and also measured the FRET efficiency and acceptor-to-donor concentration ratio of tandem constructs with different acceptor-donor stoichiometries in living HepG2 cells. mTP-G and mPb-G methods provide two simple and reliable tools for determining the G factor, in turn, quantitatively measuring FRET signal and monitoring dynamic biochemical processes in living cells.  相似文献   
3.
A highly selective dual-channel NIR fl uorescent probe (DFB1) based on curcuminoid difl uoroboron is developed for discrimination Cys over GSH, Hcy and other amino acids in mitochondria of living cells.  相似文献   
4.
Several leading manufacturers recently combined the traditional retail channel with a direct online channel to reach a wider range of customers. We examine such a dual-channel supply chain under price and delivery-time dependent stochastic customer demand. We consider five decision variables, the price and order quantity for both the retail and the online channels and the delivery time for the online channel. Uncertainty frequently arises in both retail and online channels and so additional inventory management is required to control shortage or overstock and that has an effect on the optimal order quantity, price, and lead time. We developed mathematical models with the profit maximization motive. We analyze both centralized and decentralized systems for unknown distribution function of the random variables through a distribution-free approach and also for known distribution function. We examine the effect of delivery lead time and customers’ channel preference on the optimal operation. For supply chain coordination a hybrid all-unit quantity discount along a franchise fee contract is used. Moreover, we use the generalized asymmetric Nash bargaining for surplus profit distribution. A numerical example illustrates the findings of the model and the managerial insights are summarized for centralized, decentralized, and coordinated scenarios.  相似文献   
5.
A supply chain model with direct and retail channels   总被引:3,自引:0,他引:3  
We study a dual channel supply chain in which a manufacturer sells to a retailer as well as to consumers directly. Consumers choose the purchase channel based on price and service qualities. The manufacturer decides the price of the direct channel and the retailer decides both price and order quantity. We develop conditions under which the manufacturer and the retailer share the market in equilibrium. We show that the difference in marginal costs of the two channels plays an important role in determining the existence of dual channels in equilibrium. We also show that demand variability has a major influence on the equilibrium prices and on the manufacturer’s motivation for opening a direct channel. In the case that the manufacturer and the retailer coordinate and follow a centralized decision maker, we show that adding a direct channel will increase the overall profit. Our numerical results show that an increase in retailer’s service quality may increase the manufacturer’s profit in dual channel and a larger range of consumer service sensitivity may benefit both parties in the dual channel. Our results suggest that the manufacturer is likely to be better off in the dual channel than in the single channel when the retailer’s marginal cost is high and the wholesale price, consumer valuation and the demand variability are low.  相似文献   
6.
Zhao Y  Zheng B  Du J  Xiao D  Yang L 《Talanta》2011,85(4):2194-2201
A novel rhodamine-based fluorescent chemosensor (RND) was synthesized that contains two independent fluorophores and acts as a very sensitive, selective and reversible off-on probe for Hg(II). Importantly, this newly developed sensing system exhibited different fluorescent responses toward Hg(II) and Mg(II) at 589 nm and 523 nm, respectively. RND also displayed detectable color change upon treatment with Hg(II). Results from confocal laser scanning microscopy experiments demonstrated that this chemosensor is cell permeable and can be used as a fluorescent probe for monitoring Hg(II) or Mg(II) in living cells. This probe can also indirectly detect glutathione (GSH) and cysteine (Cys) with good linear relationships.  相似文献   
7.
Dual-channel measuring method based on long-period grating in holey fiber (LPHFG) is proposed, in which cladding mode interacts with medium filled in air holes and surrounding LPHFG simultaneously. Mode coupling properties and resonance spectral response of LPHFG are numerically investigated with respect to refractive index sensitivity characteristic to the variation of medium with dual-channel measuring method. It is proved that dual-channel measuring method can enhance the index sensitivity characteristic, which can be further improved by elaborately choosing appropriate cladding mode. Dual-channel measuring method provides an excellent alternative to the index-based sensors and detectors. Cladding mode in holey fiber with special radius is very sensitive to the variation of wavelength and medium, and it can turn into high-order or low-order cladding mode. The characteristics of cladding mode to wavelength and medium could be used to optimize the index-based sensor, and it also can be used in mode conversion.  相似文献   
8.
A new type of fluorescent probe capable of detecting Ag+ and Hg2+ in two independent channels was developed in the present work. Specifically, in CH3CN–MOPS mixed solvents with CH3CN/MOPS ratio (v/v) of 15/85, this type of probe fluoresced weakly, and the addition of Ag+ remarkably induced fluorescence enhancement of the probe. In CH3CN–MOPS mixed solvents with the percentage of CH3CN increased up to 65%, the probe was highly fluorescent and addition of Hg2+ dramatically induced the fluorescence quenching. Thus, using such single-fluorophore-based probe and tuning the polarity of the mixed solvent, Ag+, and Hg2+ can be detected in independent channels with high selectivity and sensitivity. As a result, the mutual interference usually encountered in most cases of Ag+ and Hg2+ sensing owing to the similar fluorescence response that these two ions induced, can be effectively circumvented by using the probes developed herein.  相似文献   
9.
Simultaneous tracking of the vertical laryngeal position in various phonatory tasks was performed for 2 subjects (one male, one female) using dual-channel electroglottography (EGG) and videofluorography (videofluoroscopy). The tasks included phonation on [a:] with voluntary repeated (1) heightening and (2) lowering of the laryngeal position; alternation between [a:] and phonation on (3) [b], (4) [m], and (5) the voiced bilabial fricative [beta:]; and (6) production of the corner vowels [a, i, u]. EGG and videofluorography agreed about the direction of changes in the vertical laryngeal position in most cases. Most disagreements were found in the amount of changes. Reasons for the discrepancies, including, for example, changes in the resting position of the larynx and anteroposterior movements of the cartilages, are discussed. It can be concluded that dual-channel EGG is a valuable clinical and pedagogical tool for the analysis of the vertical displacement of larynx in well-specified phonatory tasks. The method should act most reliably on separately phonated vowels. Its applicability for studying laryngeal biomechanics more wholistically, however, is limited.  相似文献   
10.
《Current Applied Physics》2014,14(7):932-935
Thin film transistors (TFTs) with indium–zinc tin-oxide (IZTO) dual-channel layers were fabricated on heavily-doped p-type Si substrates by using a tilted dual-target radio-frequency magnetron sputtering system. The number of oxygen vacancies in the IZTO channel layer decreased with increasing oxygen partial pressure, resulting in a decrease in the conductivity. The threshold voltage (Vth) shifted toward positive gate-source voltage with increasing oxygen partial pressure for the growth of the IZTO layer because of a decrease in the carrier concentration. The Vth, the mobility, the on/off-current ratio, and the subthreshold swing of the dual-channel IZTO TFTs were 3.5 V, 7.1 cm2/V s, 1.3 V/decade, and 8.2 × 106, respectively, which was enhanced by utilizing dual-channel layers consisting of a top channel deposited at a high oxygen partial pressure and a bottom channel deposited at a low oxygen partial pressure.  相似文献   
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