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利用激光扫描共聚焦显微技术和碱性单细胞凝胶电泳技术对经过离子注入后的黑松花粉粒内细胞核的直接损伤效应进行了观察鉴定。 研究结果表明, 离子注入后可以直接损伤细胞核结构, 导致细胞核裂解。 细胞核的损伤程度与注入离子的剂量密切相关, 即细胞核DNA分子的损伤程度随着注入离子剂量的增加而提高。 The damage of pollen nuclei of Pinus thunbergii induced directly by ion implantation were measured by the laser confocal microscopy technique and the single cell gel electrophoresis test. The results showed that ion implantation caused the nuclei structure to be damaged, leading to the nuclei degradation. The results of statistical analysis showed that the damage grade of nuclei was very correlative with the ion dosage. The damage degree of DNA in the nuclei at the level of single cells was increased with the increase of the ion implantation dose.  相似文献   
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不同辐照源对黑松花粉粒的生物学效应   总被引:1,自引:1,他引:0  
研究了N+离子束、 紫外线和γ射线辐照对黑松花粉粒的影响, 对3种辐射源在诱发花粉粒细胞出现损伤效应上的差异性进行了比较分析。 研究结果表明, 3种辐照源对黑松花粉粒萌发和花粉管生长所表现出的效应存在明显的差异。 γ射线的剂量效应曲线表现为近S型; 紫外线辐照的剂量效应曲线呈现出近L型; 离子束的生物学效应主要在两个方面表现出特异性, 即离子束所导致的剂量 效应曲线呈“马鞍型”趋势和N+离子束注入后会诱发花粉管顶端产生出明显的肿胀现象。The effects of pollens and pollen tubes of Pinus thunbergii induced respectively by N+ beam, γ ray and ultraviolet ray were measured , and the differences of the effects caused by the different radiant factors were distinguished. The results showed that there was obvious difference in the damages of the pollen germination and the pollen tube growth led by the radiant factors. The curve of dose effects from γ ray irradiation was similarly S type, and that from ultraviolet ray treatment approximately L type . The effects from ion implantation expressed the two characteristics, the curve of the saddle type and the top inflation of pollen tube.  相似文献   
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