单个人类端粒重复序列d(TTAGGG)对长链端粒DNA-d[AGGG(TTAGGG)6]结构影响的研究

本文使用超速离心沉降速度法、聚丙烯酰胺凝胶电泳和圆二色光谱和紫外吸收检测熔融实验研究了长序列端粒DNA-d[AGGG(TTAGGG)₆](G₆-DNA)的结构以及单个重复序列DNA-d(TTAGGG)(G₀₁-DNA)对G₆-DNA结构的影响. 结果表明G₆-DNA可以在水溶液中形成二聚体，并且G₀₁-DNA可以通过与G₆-DNA结合形成额外的G-四链体结构，从而改变单体与二聚体的平衡. 然而，G₀₁-DNA对序列为d[AGGG(TTAGGG)₃](G₃-DNA)的结构没有影响. 该研究为长序列单链端粒DNA的结构多样性提供了新的见解.     

一种新型含炔基的聚合2-乙基-2-噁唑啉引发剂的合成

A novel trifunctional initiator with one alkyne and two trifluoromethanesulfonate moieties was synthesized from a protected alcohol 5-hydroxyl-2-phenyl-1, 3-dioxane. The alkyne functionalized intermediate with two protected alcohol groups was synthesized by reacting with propargyl bromide. The alcohol groups were cleaved using a mixture of tetrahydrofuran and hydrochloric acid aqueous solution. In the last step the initiator was synthesized using triflic anhydride in carbon tetrachloride. The initiator was characterized by ¹H NMR and used for the polymerization of 2-ethyl-2-oxazoline which gives polymers with narrow distribution. For comparison a similar initiator with two tosylates was prepared and used for the polymerization of the monomer 2-ethyl-2-oxazoline, the resulting product has a wide molecular weight distribution and most of the initiator remains unreacted after 24 h which may be due to the steric hindrance between the two tosylate groups. To further explore the steric hindrance phenomenon, a linear tosylate initiator was synthesized, but still some of the initiator remains unreacted, illustrating that both steric hindrance and electrophilic balance affect the efficiency of the cationic ring-opening polymerization. All of the polymers were characterized in detail by using ¹H NMR, matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy, and size exclusion chromatography to confirm the purity and distribution of the polymers.