~(12)C~(6+)高能重离子辐照大葱损伤及其分子生物学效应

利用30,90,180Gy3种剂量的12C6+重离子束辐照大葱种子,研究其在细胞水平和农艺性状的诱变效应并进行RAPD分析。通过与M1代的研究结果比较后表明:经过不同剂量12C6+重离子照射后能有效地诱导大葱细胞形成微核和染色体畸变,这种诱变效应,在M2代仍然有所表现。M1代大葱结果期的株高、白长、花序直径和种子产量随辐照剂量增加产生明显差别,其中30Gy辐照组增幅最大。大葱总水溶性蛋白质和维生素C的含量在30Gy组中积累最多,在90Gy组有明显下降。与M1代一致,M2代中大葱染色体微核率及RAPD分析所得的DNA多态性比率仍然与辐照剂量呈正相关,但比率整体下降;说明高能量重离子辐照造成的DNA变异在M2代被修复和淘汰。     

12C6+离子辐照对4种农作物种子出苗率

以能量为80 MeV/u的12C6+离子为诱变源辐照油菜、 胡麻、 大葱和兵豆的干种子后， 研究了不同剂量处理对4种农作物M1和M2代种子出苗率及幼苗生长的影响。 实验结果表明: 重离子所导致的M1代生物学效应因不同的物种而表现出一定的差异， 适当剂量C离子辐照促进了油菜和胡麻M1代出苗率和幼苗的生长； 而不同剂量的C离子辐照抑制了大葱的出苗率和幼苗的生长； 兵豆3个剂量下的出苗率和对照相差很小， 但90 Gy辐照有利于其生长。 到了M2代， 4种作物辐照组的发芽率都低于各自的对照组； 30 Gy剂量下的油菜、 胡麻和兵豆长势最好； 大葱依然是对照的长势最好。 Crops of Brassica napus L.， Linum usitatissmum L.， Allium fistulosum L. and Lens culinaris Medic. were irradiated by 80 MeV/u 12C6+ ion beams with doses of 30， 90 and 180 Gy. The germination rates and heights of seedlings of M1 and M2 generation of these four plants were studied. The results indicated that germination rates and average heights of the B. napus and L. usitatissmum were improved by appropriate dose treatment， while great suppression was found in the irradiated groups of the A. fistulosum. As far as the L. Culinaris was concerned， little differences was observed on M1 germination rate， but the 90 Gy irradiation was favorable to growth of plant. The treatments with 30， 90 and 180 Gy were inferior to contrast one on M2 germination rate of the four species. Seedlings of M2 generation of the B. napus， L. sitatissmum and L. culinaris under 30 Gy grew better than the other groups， while the best performance of the A. fistulosum was shown by the control group.