两种保护剂对C离子诱导小鼠急性肝损伤的应答

比较了N-乙酰半胱氨酸(NAC)及乙酰左旋肉毒碱(ALCAR)对12C6+离子照射小鼠的损伤效应,并探讨了其可能的作用机制。利用4Gy剂量的12C6+离子束对预先给予NAC(100mg/kg)和ALCAR(100mg/kg)保护的昆明小鼠进行单次全身照射。随后检测肝组织中总抗氧化能力(TAC)、DNA单链断裂和细胞凋亡率。结果显示,与照射对照组相比,提前给予NAC和ALCAR均极显著地增强了肝组织的抗氧化能力(P0.001),减轻了12C6+离子导致的肝组织中DNA断裂(P0.001)和细胞凋亡(P0.001)。此外,还发现ALCAR组抗重离子辐照损伤的能力显著地高于NAC组(P0.05)。实验结果提示了NAC和ALCAR可通过抵御组织内的氧化胁迫,阻止DNA链的断裂和细胞的凋亡,实现对C离子辐照损伤的保护效应。而且ALCAR比NAC可能更适合成为有潜力、有希望的抗C重离子辐射药物。     

12C6+离子预辐射对小鼠胸腺脾脏细胞周期进程的影响

以0， 0.05， 0.1， 0.25， 0.5 Gy 12C6+ 离子全身预辐照昆明小鼠， 间隔4 h后再对小鼠进行4 Gy全身辐射。 辐照后12 h用流式细胞仪检测小鼠胸腺脾脏细胞在各细胞周期时相的百分率， 同时用单细胞电泳检测受辐射小鼠胸腺脾脏细胞DNA损伤程度。 结果显示， 相对于大剂量预照射组， 各低剂量预照射组胸腺细胞S期细胞百分率显著减少； 脾脏细胞G0/G1期细胞百分率明显减少； 同时胸腺脾脏细胞的拖尾率及拖尾长度明显减少， 以0.1 Gy预辐照效果最为明显。 这些结果表明， 低剂量预辐射处理可以减弱胸腺细胞的S期阻滞及脾脏细胞的G1期阻滞， 并明显减轻胸腺脾脏细胞的DNA损伤程度。     

辐射诱导促进AdCMV-p53 转染前列腺癌细胞

用腺病毒重组体（AdCMV p53/GFP）转染经0.5, 1.0和2.0 Gy γ射线辐射处理的前列腺癌细胞[PC 3（ nullp53）]， 用克隆形成法检测细胞增殖能力， 用流式细胞分析法测定腺病毒重组体转染率和外源性p53蛋白表达。 结果提示， 辐射诱导使腺病毒重组体转染PC 3细胞提高7%—39%。 辐射联合 AdCMV p53 转染组p53表达水平提高18.5%—35.4%。 与单纯 AdCMV p53 转染组和单纯辐射组相比， 辐射联合 AdCMV-p53 转染组细胞存活率分别降低25%—64%和22%—65%。 To determine whether low dose pre irradiation could enhance adenovirus mediated p53 transfer and expression in human prostate adenocarcinoma， the PC 3 cells were pre exposed to γ rays， and then infected with replication deficient adenovirus recombinant vectors， containing human wild type p53 (AdCMV p53) or green fluorescent protein gene (AdCMV GFP) respectively (γ ray irradiation + AdCMV p53 /GFP infection). The exogenous gene transfer and expression were detected by flow cytometric analysis. The GFP transfer frequencies in γ irradiation + AdCMV GFP infection groups were 7%—39% more than those in AdCMV GFP infection groups. The p53 levels in the γ irradiation + AdCMV p53 infection groups were 18.5%—35.4% more than those in AdCMV p53 infection groups (p<0.05)，suggesting that low dose (less than or equal to 1.0 Gy) irradiation could significantly promote exogenous p53 transfer and expression in the PC 3 cells. The survival fractions for the γ irradiation + AdCMV p53 infection groups were 25%—64%， 22%—65% less than those for AdCMV p53 infection， or γ irradiation groups， respectively (p<0.05).     

甘肃当归新品系DGA2000-02的选育研究

当归新品系DGA2000 02是采用重离子束55 MeV/u 40Ar+15 离子辐照甘肃当归90 01干种子， 按新品种选育程序多年选育而成的。 2005—2007年， 在定西市岷县、 渭源县、 漳县、 陇西县等地当归品系区域试验中， 甘肃当归DGA2000 02平均产鲜当归10 621.5 kg/hm2， 较对照品种（甘肃当归90 01）平均增产鲜当归1 386.0 kg/hm2， 增产率15.0%。 生育期790 d， 茎秆深紫色， 根系黄白色。 测定结果: 总灰分4.2%， 酸不溶性灰分0.4%， 分别优于对照品种16%和33.3%; 浸出物61.4%， 较规定指标提高4.4%; 阿魏酸0.148%， 较规定指标提高2.96倍。 质量显著优于对照品种和2005年版《中国药典》规定指标。 适宜在海拔2 000—2 600 m 、 年降水量500—600 mm的二阴及高寒阴湿生态区栽培。 Angelica sinensis（Oliv）Diels new strain DGA2000 02 was selected successfully by Institute of Modern Physics， Chinese Academy of Sciences， Dryland Farming Research and Extension Center of Dingxi Prefecture， etc. According to the program of new strain selection， this new strain was selected for several years after the dry seeds of Gansu Angelica sinensis（Oliv）Diels 90 01 was irradiated by ions of 55 MeV/u 40Ar+15. During the period of year 2005—2007， region experiments of Angelica sinensis（Oliv）Diels new strain DGA2000 02 were developed in Minxian， Weiyuan， Zhangxian and Longxi etc. Average yield of the fresh DGA2000 02 Angelica was 10 621.5 kg/hm2， and 15.0% production was increased more than control (for 1 386.0 kg/hm2 of 90 01).The growth stage of the DGA2000 02 was 790 d， and it has deep purple stem and yellow white root. The quality analysis results are as follows: total ash content is 4.2% and acid fast ash content is 0.4%， 16% and 33.3% better than control， respectively; the lixivium is 61.4%， i.e., 4.4% more than the standard of Pharmacopoeia of People’s Republic of China (2005 edition); the ferulic acid content is 0.148%， i.e., 2.96 times ligher than the standard. All these results showed that the quality of the DGA2000 02 was better significantly than both control and the standard. It can be grown appropriately at the high， cold and dankness regions at the altitude of 2 000—2 600 m and with a annual precipitation of 500—600 mm.     

重离子束定点诱变育种初探

介绍了在兰州重离子加速器上采用 75 Me V/u16O8+离子进行了贯穿与定点注入的实验 ,以及不同注入部位的种子在实验室萌发的根尖细胞染色体的微核率和畸变率与大田培育结果随剂量的变化情况 .经过 3年 5代 (南繁加代 )的系统选育 ,筛选出增产、矮杆、抗 (锈 )病、抗干热风和早熟等 9个稳定突变系.Penetration and site chosen implantation of spring wheat seeds at HIRFL with 75 MeV/u 16 O 8+ ions were caried out. The seeds, of which the different sites ware implanted by the ions were germinated in room. The frequency of micronuclei and chromosome aberration in their root tip cells was observed. The results of their cultivation in the field were different. Through selection of three year five generations (adding a generation in southern China each year), nine stable...