ESTABLISHMENT AND BIOLOGICAL PROPERTIES OF MOUSE GRANULOCYTIC LEUKEMIC CELL LINE-L_(833)

A granulocytic Leukemic cell line, called L_(833), has been established through culturing in vitro frommouse bone marrow of transplantable granulocytic leukemia. More than 280 passages have been performedin 3 years. Cell growth has been rapid and stable. Incidence of tumour formation was 100% with variousroutes of inoculation. The nature of granulocytic leukemia was confirmed by examination of cytology,cytochemistry, pathology and ultrastructural changes. Analysis proved chromosomes to be hypodiploid with model number of 39, loss of chromosomes, andpresence of a marker. Besides the chromosomal change as mentioned above, both L_(883-A) and L_(833-B)derived from colonies formed from the L_(833) cells cultuerd in semi-solid agar medium, have their ownmarker. The cell line was sensitive to various types of antitumour agents in varying degrees. It was alsosensitive to ionizing radiation. D_0 values of L_(833) and L_(833-A) cells were 98.8 and 104.9 rad, respec-tively. The results were similar to that of L_(8     

小鼠粒细胞白血病细胞体外细胞系L_(833)的建立及其生物学特性的研究

本文用小鼠可移植性粒细胞白血病瘤株L_(801)的骨髓细胞,经体外培养建立了粒细胞白血病细胞系(株),定名为L(833)。三年来在体外已传至280余代,细胞生长迅速稳定,经不同途径动物接种致瘤率均为100％。经细胞学、细胞化学、病理学和超微结构等检查,均证明本细胞系(株)仍为粒细胞白血病细胞。L_(833)细胞系细胞染色体分析证明仍然是亚二倍体,众数39条,Y染色体丢失和存在一个标记染色体。L_(833)-A和L_(833)-B(利用半琼脂法分选出的细胞集落定名为A和B)染色体除了上述变化外,还各自存在一个标记染色体。本系细胞对所用各类抗癌药物均有不同程度的敏感性。对辐射(γ线)也很敏感,L_(833)和L_(833)-A的D_0值分别为98.8和104.9 rad。其结果和L_(801)基本是一致的,它的建立不但对于深入研究白血病和其药物筛选等有重要意义,而且利用体外瘤株进行有关研究更直观、经济和易于进行精确定量研究。     

叶腊石(Pyrauxite)表面活性提高的机理

采用振动磨对叶腊石进行超细粉磨,并试用助剂与叶腊石共磨工艺技术,得到了表面活性较高的粉磨产品,分析并讨论了加入助剂前后的粉磨工艺效果,并对一面活性提高的机理进行了探讨。     

逆向热蒸发制备望远镜主镜银反射膜

以自上向下热蒸发大型镀膜机为实验平台,开展了逆向蒸发制备大口径银反射镜研究.通过设计特殊蒸发舟及优化相关工艺参数,实现了金属银膜稳定、高速向下蒸发.根据逆向热蒸发工艺特点,选取熔点低、升华型材料Cr和SiO及特殊设计的蒸发舟,以Cr或CrNx为粘结层,SiOx或SiOxNy为保护层,制备了三种具有四层结构的介质保护性银反射膜,并对所制备反射镜样品的反射率、断面形貌和环境稳定性进行了表征.实验结果表明:膜系结构为3 nmCr/120 nmAg/0.6 nmCrNx/150 nmSiOxNy的银反射膜具有较好的环境稳定性和光谱特性;通过了24小时湿热实验(50℃/相对湿度98％/)和盐雾实验,在400～1 800 nm波段光谱范围内,银反射膜的平均反射率达到97.8％.     

Anti-Reflective Fluoride Coatings for Widely Tunable Deep-Ultraviolet Diode-Pumped Solid-State Laser Applications

An anti-reflective （AR） fluoride coating in the 170-230 nm spectral range is prepared by the thermal evaporation method for the applications of widely tunable deep-ultraviolet diode-pumped solid-state lasers. The transmittance of an AR coated calcium fluoride （CaF2） window in thickness 3mm is measured to be in the range of 95.8% at 170 nm to 97.1 % at 230 nm, with the maximum transmittance 99.2% and the minimum residual reflectance 0.04% appeared at 195 nm. The experimental results indicate that treating the AR coated window and the bare substrate with ultraviolet irradiation can significantly improve their optical performance.     

磁镜场对射频等离子体中离子能量分布的影响

运用阻碍栅极型能量分析器,在不同磁镜场参数下测量了低温等离子体中离子的能量分布. 结果表明,在放电管中心处离子能量分布,随磁镜场强度的增加而向低能方向偏移,但离子能量分布宽度却没有明显的变化;而随着磁镜比的增加离子能量分布却向高能方向偏移,并且离子能量分布宽度也将变宽.由此可见,磁镜场参数对离子能量分布有很大影响. 关键词： 磁镜场 辉光放电 低温等离子体 离子能量分布     

SiOx protective film thermally evaporated downwards for 2-m level primary mirror

The service life of the large primary mirror with aluminum coating can be effectively prolonged by a protective layer. The SiOx （1〈x〈2） protective material which is thermally evaporated from up to bottom is studied. Environmental adaptability experiment, spectral measurement and micro-morphology analysis are executed on bare SiOx coatings within different oxygen concentrations, and the repeatability verification is implemented by testing the SiOx protected aluminum coatings. The results show that the SiOx coatings can meet the protective qualification within oxygen flow of 50 sccm （p=5.6× 10-3 Pa）. The fine compactness of the coating has excellent moisture resistance with the average molecular spacing of 0.335 nm. In addition, the average reflectivity of the SiOx protected aluminum coating is 90.47% in the band 400 --2 500 nm and the coating is confirmed to have good environmental performance.     

氧化物激光薄膜的离子束溅射制备技术

 研究了由Ta₂O₅和SiO₂组成的多层氧化物激光薄膜的双离子束溅射制备工艺。简要介绍了离子束溅射技术的基本工作原理和应用，着重分析了薄膜厚度均匀性的调控方法。先后得到了Ta₂O₅和SiO₂单层薄膜厚度均匀性调控结果以及不同波长处薄膜折射率，并定性地分析比较了离子束溅射和电子束蒸发制备的薄膜结构；制备并测试了633nm,1 315nm反射薄膜以及增透膜。结果表明：采用离子束溅射技术能够制备出优良的、满足需要的激光高反射薄膜元件。     

大型天文望远镜主镜镀膜技术

采取主镜镜面向上真空蒸镀制备方式,开展了大型天文望远镜主镜铝加介质保护反射膜技术研究。结合ZZS3200型镀膜设备真空室几何配置,对传统膜厚分布计算物理模型进行了修正,分别分析了铝膜与介质膜层膜厚均匀性。结果表明:理论分析与实验数据一致,直径2.4 m范围内膜厚不均匀性约5.5%。通过优化工艺,成功制备出1.8 m平面镜薄膜元件,在350~1 800 nm波段范围内,膜层平均反射率88.6%,通过了环境稳定性测验。