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1.
Time-resolved fluorescence resonance energy transfer (FRET) measurements were used to measure distance distributions and intramolecular dynamics (site-to-site diffusion) of a 28-residue single-domain zinc finger peptide in the absence and presence of zinc ion. Energy transfer was measured between TRP14 and a N-terminal DNS group. As expected, the TRP-to-DNS distance distribution for zinc-bound peptide is shorter and narrower (R av=11.2 Å,hw=2.8 Å) than the metal-free peptide (R av=20.1 Å,hw=14.5 Å). The degree of mutual donor-to-acceptor diffusion (D) was also determined for these distributions. For zinc-bound peptide there is no detectible diffusion (D0.2 Å2/ns), whereas for metal-free peptide a considerable amount of motion is occurring between the donor and the acceptor (D=12 Å2/ns). These results indicate that the zinc-bound peptide folds into a unique, well-defined conformation, whereas the metal-free conformation is flexible and rapidly changing. The absence of detectible mutual site-to-site diffusion between the donor and the acceptor in the metal-bound zinc finger peptide indicates that intramolecular motion is essentially frozen out, on the FRET time scale, as a consequence of zinc coordination.Dedicated to the memory of Barbara D. Wells.  相似文献   
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研究了4度循环图,构造出其任意两点之间的四条内部点不交路,并且给出其宽直径的一个较好的上界。  相似文献   
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微光成像系统的阈值探测理论和视距探测方程的研究   总被引:6,自引:2,他引:6  
艾克聪 《应用光学》1994,15(4):21-28
研究目前文献中常见的18种微光成像系统探测方程之间的内在联系和异同之处,分析论证各种阈值探测理论和探测方程存在的问题和不足。在此基础上,从人眼的视觉理论和阈值特性出发,建立微光成像系统新的阈值探测理论模型,并分别导出微光直视系统和微光电视系统的视距探测方程。应用所导出的理论公式计算的数据与各种实际系统的实测数据进行了比较,证明新的阈值探测理论和视距探测方程是正确可靠的,且具有很高的精度。  相似文献   
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The encoding process of finding the best-matched codeword (winner) for a certain input vector in image vector quantization (VQ) is computationally very expensive due to a lot of k-dimensional Euclidean distance computations. In order to speed up the VQ encoding process, it is beneficial to firstly estimate how large the Euclidean distance is between the input vector and a candidate codeword by using appropriate low dimensional features of a vector instead of an immediate Euclidean distance computation. If the estimated Euclidean distance is large enough, it implies that the current candidate codeword could not be a winner so that it can be rejected safely and thus avoid actual Euclidean distance computation. Sum (1-D), L2 norm (1-D) and partial sums (2-D) of a vector are used together as the appropriate features in this paper because they are the first three simplest features. Then, four estimations of Euclidean distance between the input vector and a codeword are connected to each other by the Cauchy–Schwarz inequality to realize codeword rejection. For typical standard images with very different details (Lena, F-16, Pepper and Baboon), the final remaining must-do actual Euclidean distance computations can be eliminated obviously and the total computational cost including all overhead can also be reduced obviously compared to the state-of-the-art EEENNS method meanwhile keeping a full search (FS) equivalent PSNR.  相似文献   
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We study time dependence of various measures of entanglement (covariance entanglement coefficient, purity entanglement coefficient, normalized distance coefficient, entropy coefficients) between resonantly coupled modes of the electromagnetic field in ideal cavities with oscillating boundaries. Two types of cavities are considered — a three-dimensional cavity possessing eigenfrequencies 3 = 31, whose wall oscillates at the frequency w = 21, and a one-dimensional (Fabry–Perot) cavity with an equidistant spectrum n = n1 where the distance between perfect mirrors oscillates at the frequencies 1 and 21. The behavior of entanglement measures in these cases turns out to be completely different, although all three coefficients demonstrate qualitatively similar time dependences in each case (except some specific situations where the covariance entanglement coefficient based on traces of covariance submatrices seems to be essentially more sensitive to entanglement than other measures, which are based on determinants of covariance submatrices). Different initial states of the field, namely, vacuum, squeezed vacuum, thermal, Fock, and even/odd coherent states, are considered.  相似文献   
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We proposed a method to find the community structure in a complex network by density-based clustering. Physical topological distance is introduced in density-based clustering for determining a distance function of specific influence functions. According to the distribution of the data, the community structures are uncovered. The method keeps a better connection mode of the community structure than the existing algorithms in terms of modularity, which can be viewed as a basic characteristic of community detection in the future. Moreover, experimental results indicate that the proposed method is efficient and effective to be used for community detection of medium and large networks.  相似文献   
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The symplectic, optical, and photon-number tomographic symbols of binomial states of the radiation field are studied. Explicit relations for all tomograms of the binomial states are obtained. Two measures for nonclassical properties of these states are discussed.  相似文献   
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Headphone rendering of nearby virtual sound sources represents to date an open issue in 3-D audio, due to a number of technical challenges and temporal requirements involved in the measurement of individual Head-Related Transfer Functions (HRTFs). In order to tackle this problem, we propose a filter model of near-field effects based on the Distance Variation Function (Kan et al., 2009). Thanks to its simple structure and low order, the model can be applied to any far-field virtual auditory display to yield a realistic and computationally efficient near-field compensation of spectral and binaural effects. The model is subjectively evaluated in two psychophysical experiments where the relative distance of pairs of virtually rendered sound sources is judged. Results show that even though sound intensity overshadows subtler near-field effects when it is available as a cue for distance, the model is capable of offering relative distance information of near lateral virtual sources when intensity cues are removed. Furthermore, performances of the model in relative distance rendering are compared to those of alternative near-field rendering methods available in the literature.  相似文献   
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