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1.
Lateral flow immunoassay (LFIA) is a rapid, simple, and inexpensive point-of-need method. A major limitation of LFIA is a high limit of detection (LOD), which impacts its diagnostic sensitivity. To overcome this limitation, we introduce a signal-enhancement procedure that is performed after completing LFIA and involves controllably moving biotin- and streptavidin-functionalized gold nanoparticles by electrophoresis. The nanoparticles link to immunocomplexes forming multilayer aggregates on the test strip, thus, enhancing the signal. Here, we demonstrate lowering the LOD of hepatitis B surface antigen from approximately 8 to 0.12 ng mL−1, making it clinically acceptable. Testing 118 clinical samples for hepatitis B showed that signal enhancement increased the diagnostic sensitivity of LFIA from 73 % to 98 % while not affecting its 95 % specificity. Electrophoresis-driven enhancement of LFIA is universal (antigen-independent), takes two minutes, and can be performed by an untrained person.  相似文献   
2.
We discuss bifurcation of periodic orbits in discontinuous planar systems with discontinuities on finitely many straight lines intersecting at the origin and the unperturbed system has either a limit cycle or an annulus of periodic orbits. Assume that the unperturbed periodic orbits cross every switching line transversally exactly once. For the first case we give a condition for the persistence of the limit cycle. For the second case, we obtain the expression of the first order Melnikov function and establish sufficient conditions on the number of limit cycles bifurcate from the periodic annulus. Then we generalize our results to systems with discontinuities on finitely many smooth curves. As an application, we present a piecewise cubic system with 4 switching lines and show that the maximum number of limit cycles bifurcate from the periodic annulus can be affected by the position of the switching lines.  相似文献   
3.
The limit cycle oscillation (LCO) behaviors of control surface buzz in transonic flow are studied. Euler equations are employed to obtain the unsteady aerodynamic forces for Type B and Type C buzz analyses, and an all-movable control surface model, a wing/control surface model and a three-dimensional wing with a full-span control surface are adopted in the study. Aerodynamic and structural describing functions are used to deal with aerodynamic and structural nonlinearities, respectively. Then the buzz speed and buzz frequency are obtained by V-g method. The LCO behavior of the transonic control surface buzz system with linear structure exhibits subcritical or supercritical bifurcation at different Mach numbers. For nonlinear structural model with a free-play nonlinearity in the control surface deflection stiffness, the double LCO phenomenon is observed in certain range of flutter speed. The free-play nonlinearity changes the stability of LCOs at small amplitudes and turns the unstable LCO into a stable one. The LCO behavior is dominated by the aerodynamic nonlinearity for the case with large control surface oscillation amplitude but by the structural nonlinearity for the case with small amplitude. Good agreements between LCO behaviors obtained by the present method and available experimental data show that our study may help to explain the experimental observation in wind tunnel tests and to understand the physical mechanism of transonic control surface buzz.  相似文献   
4.
研究集值逆鞅(集值逆上鞅)在Kuratowski收敛意义,Kuratowski-Mosco收敛意义及弱收敛意义下的收敛定理.  相似文献   
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利用求和法,在矩条件下,给出分组ρ*-混合随机场的强极限定理。它是经典结果的推广。  相似文献   
7.
We develop the rough path counterpart of Itô stochastic integration and differential equations driven by general semimartingales. This significantly enlarges the classes of (Itô/forward) stochastic differential equations treatable with pathwise methods. A number of applications are discussed.  相似文献   
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Nonlinear effects such as friction and freeplay on the control surfaces can affect aeroelastic dynamics during flight. In particular, these nonlinearities can induce limit cycle oscillations (LCO), changing the system stability, and because of this it is essential to employ computational methods to predict this type of motion during the aircraft development cycle. In this context, the present article presents a matrix notation for describing the Hénon’s method used to reduce errors when considering piecewise linear nonlinearities in the numerical integration process. In addition, a new coordinate system is used to write the aeroelastic system of equations. The proposal defines a displacement vector with generalized and physical variables to simplify the computational implementation of the Hénon’s technique. Additionally, the article discusses the influence of asymmetric freeplay and friction on the LCO of an airfoil with control surface. The results show that the extended Hénon’s technique provides more accurate LCO predictions, that friction can change the frequency and amplitude of these motions, and the asymmetry of freeplay is important to determine the LCO behavior.  相似文献   
10.
A robust 4,8-connected Tb-based metal-organic framework (Tb-MOF) with paddle wheel-shaped {Tb2(COO)4} subunits extended by C3-symmetric 3,3',3''-[1,3,5-benzenetriyltris(carbonylimino)]tris-benzoate connector was hydrothermally synthesized, showing highly environmental stability, good dispersion and intense green emission in water system. Resulting critically from the well suppressed absorption towards the excitation energy, the Tb-MOF exhibits rapid and efficient fluorescent response towards nitroimidazole antibiotics with strong quenching constants and low detection limits of 1.59 × 104 m –1 and 2.4 μM for metronidazole as well as 1.62 × 104 m –1 and 2.9 μM for dimetridazole. Moreover, the sensitive and selective identification of the Tb-MOF has strong anti-interference and excellent regeneration ability, which endows the promising applications of the Tb-MOF as fluorescent sensing materials.  相似文献   
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