Ultrafast Fluorescence Spectroscopy via Upconversion and Its Applications in Biophysics

In this review, the experimental set-up and functional characteristics of single-wavelength and broad-band femtosecond upconversion spectrophotofluorometers developed in our laboratory are described. We discuss applications of this technique to biophysical problems, such as ultrafast fluorescence quenching and solvation dynamics of tryptophan, peptides, proteins, reduced nicotinamide adenine dinucleotide (NADH), and nucleic acids. In the tryptophan dynamics field, especially for proteins, two types of solvation dynamics on different time scales have been well explored: ~1 ps for bulk water, and tens of picoseconds for “biological water”, a term that combines effects of water and macromolecule dynamics. In addition, some proteins also show quasi-static self-quenching (QSSQ) phenomena. Interestingly, in our more recent work, we also find that similar mixtures of quenching and solvation dynamics occur for the metabolic cofactor NADH. In this review, we add a brief overview of the emerging development of fluorescent RNA aptamers and their potential application to live cell imaging, while noting how ultrafast measurement may speed their optimization.     

VISCOPLASTIC SOLUTION TO FIELD AT STEADILY PROPAGATING CRACK TIP IN LINEAR- HARDENING MATERIALS

An elastic-viscoplastic constitutive model was adopted to analyze asymptotically the tip-field of moving crack in linear-hardening materials under plane strain condition. Under the assumption that the artificial viscosity coefficient was in inverse proportion to power law of the rate of effective plastic strain, it is obtained that stress and strain both possess power law singularity and the singularity exponent is uniquely determined by the power law exponent of the rate of effective plastic strain. Variations of zoning structure according to each material parameter were discussed by means of numerical computation for the tip-field of mode Ⅱ dynamic propagating crack, which show that the structure of crack tip field is dominated by hardening coefficient rather than viscosity coefficient. The secondary plastic zone can be ignored for weak hardening materials while the secondary plastic zone and the secondary elastic zone both have important influence on crack tip field for strong hardening materials. The dynamic solution approaches to the corresponding quasi-static solution when the crack moving speed goes to zero, and further approaches to the HR (Hui-Riedel) solution when the hardening coefficient is equal to zero.     

泡沫铝夹芯双圆管结构的准静态轴向压缩性能研究

对泡沫铝夹芯双圆管结构的准静态轴压性能进行了实验研究,发现该新型结构的比质量吸能效率远远高于传统的泡沫铝夹芯单管,并接近甚至超过相应的空管结构;其内外管变形模式均与空管不同且受内外管组合的影响.本文讨论了它的变形机理,分析了外管壁厚对其压缩行为的影响,发现增大外管壁厚有利于增大结构的行程利用率,提高结构的比质量能量吸收...     

Mechanical model of lung parenchyma as a two-phase porous medium

The anatomy and geometry of the lung at the micro- and macroscopic level have been described briefly. A notion of lung parenchyma — a macroscopically continuous medium whose mechanical properties result from those of microstructural components — has been adapted. Simplifying assumptions propounded in the constitutive model have been discussed. Two phases have been distinguished in the medium: the solid phase — a highly deformable, nonlinearly elastic skeleton in the form of a thin-walled tissue structure on the micro-scale — and the fluid phase — perfect gas (air) filterating through the structure. General constitutive relations for both phases and their mechanical interactions have ben formulated. Further, the fundamental set of differential equations of the quasi-static coupled problem has been developed. Large deformations, material nonlinearities, and dependence of permeability on skeleton deformation have been included. Matrix formulation of the problem has been presented from the point of view of the finite element method. An implicit iterative time integration scheme has been proposed. The algorithm has been illustrated with results of simple numerical tests.     

移动荷载作用下地基动力分析的有限元方法

通过对地基动力问题的基本方程进行变换,把基本方程变换到随荷载移动的运动坐标系中,通过加权残数法推导了相应的单元刚度矩阵,从而建立了移动问题的有限元格式,并发现移动荷载问题的单元刚度矩阵是对相应静力问题单元刚度矩阵的修正,在静力单元刚度矩阵的主对角元素上增加与移动速度有关的项,即可得到移动问题有限元的单元刚度矩阵,这样就将动力学问题转化为“拟静力”问题处理。文中用移动问题有限元方法计算了地基的动力响应,并与解析解进行了对比,以说明本方法具有较好的精度。     

泡沫铝夹芯双方管结构准静态轴压性能的实验研究

对几种泡沫铝夹芯方管结构的准静态轴压性能进行了实验研究。结果表明,较空管及泡沫铝夹芯单管结构,泡沫铝夹芯双管结构的承载能力及能量吸收效率明显得到增强。双管夹芯结构中外管撕裂模式的结构行程利用率和能量吸收效率高于相应的外管周期折叠模式。而泡沫铝四角填充方式提高了双管夹芯结构相同变形模式下的能量吸收效率和结构变形的稳定性。同时研究了内管管壁及材料强度对泡沫铝夹芯双管结构的影响。随着内管壁厚增大,双管夹芯结构的能量吸收效率提高,而内管材料强度影响不明显。     

Dynamic and quasi-static bending of saturated poroelastic Timoshenko cantilever beam

Based on the three-dimensional Gurtin-type variational principle of the incompressible saturated porous media, a one-dimensional mathematical model for dynamics of the saturated poroelastic Timoshenko cantilever beam is established with two assumptions, i.e., the deformation satisfies the classical single phase Timoshenko beam and the movement of the pore fluid is only in the axial direction of the saturated poroelastic beam. Under some special cases, this mathematical model can be degenerated into the Euler-Bernoulli model, the Rayleigh model, and the shear model of the saturated poroelastic beam, respectively. The dynamic and quasi-static behaviors of a saturated poroelastic Timoshenko cantilever beam with an impermeable fixed end and a permeable free end subjected to a step load at its free end are analyzed by the Laplace transform. The variations of the deflections at the beam free end against time are shown in figures. The influences of the interaction coefficient between the pore fluid and the solid skeleton as well as the slenderness ratio of the beam on the dynamic/quasi-static performances of the beam are examined. It is shown that the quasi-static deflections of the saturated poroelastic beam possess a creep behavior similar to that of viscoelastic beams. In dynamic responses, with the increase of the slenderness ratio, the vibration periods and amplitudes of the deflections at the free end increase, and the time needed for deflections approaching to their stationary values also increases. Moreover, with the increase of the interaction coefficient, the vibrations of the beam deflections decay more strongly, and, eventually, the deflections of the saturated poroelastic beam converge to the static deflections of the classic single phase Timoshenko beam.     

反复荷载下型钢再生混凝土组合柱粘结性能研究

为研究反复荷载下型钢再生混凝土组合柱粘结性能,对17个组合柱进行了拟静力试验。重点分析型钢翼缘应变分布特征,利用粘结应力计算公式获取型钢翼缘粘结应力大小及其分布规律。在此基础上,分析组合柱粘结破坏机理和位移荷载循环次数对其粘结应力的影响规律,并给出粘结应力退化率计算公式。研究结果表明:反复荷载作用下型钢再生混凝土组合柱粘结应力分布规律基本呈抛物线状;柱中部附近的粘结应力最大,柱上下端粘结应力分布复杂,需对这三个部位采取相应的措施以保证型钢与再生混凝土之间的粘结强度;位移循环荷载作用下粘结强度逐渐降低,出现粘结应力退化现象。研究结论可为型钢再生混凝土组合柱的抗震设计提供理论参考。     

MDYB-3有机玻璃在不同应变率下的一维屈服行为

对MDYB-3有机玻璃进行了多组不同应变率(10-3~3 000 s-1)下的压缩实验, 得到准静态下的屈服应力与动态下的峰值应力。沿其增强与面内2个方向进行准静态压缩实验, 以分析定向拉伸对屈服应力的影响, 修正了Ree-Eyring模型与Cooperative模型以描述定向有机玻璃的屈服行为。采用Johnson-Cook模型描述屈服后的黏塑性行为。结果表明Cooperative屈服模型比Ree-Eyring屈服模型更接近实验结果, 且能准确描述准静态屈服应力。动态压缩下的峰值应力为失效应力, 说明试样在1 500 s-1以上应变率下未达到屈服应力时已经发生破坏。Johnson-Cook模型对于单条曲线拟合良好, 但无法准确描述材料的应变率相关性。     

颗粒堆准静态崩塌及慢速流动过程中的堆结构研究

运用自行设计的实验装置并结合颗粒跟踪与成像技术,系统地研究了不同堆积程度的颗粒堆在准静态崩塌过程中的滑移行为.实验发现在颗粒堆中一部分颗粒沿着斜下方向滑动,而另一部分颗粒则几乎没有滑动.精确的数据分析还表明颗粒堆的滑动部分具有明显的层状结构特征,在每一滑动层中颗粒的滑移量基本相同,且各滑动层之间近似相互平行.这些滑动层与水平面的夹角约在40—50之间,与静态层表面与水平面的夹角一致.各滑动层中颗粒的滑移量随其距静态层表面的距离而线性的增加.不同堆积程度的颗粒堆的静态部分基本上不随堆积体积而改变.