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1.
Present and future of surface plasmon resonance biosensors   总被引:21,自引:0,他引:21  
Surface plasmon resonance (SPR) biosensors are optical sensors exploiting special electromagnetic waves—surface plasmon-polaritons—to probe interactions between an analyte in solution and a biomolecular recognition element immobilized on the SPR sensor surface. Major application areas include detection of biological analytes and analysis of biomolecular interactions where SPR biosensors provide benefits of label-free real-time analytical technology. This paper reviews fundamentals of SPR affinity biosensors and discusses recent advances in development and applications of SPR biosensors.  相似文献
2.
A 21st century technique for food control: Electronic noses   总被引:17,自引:0,他引:17  
This work examines the main features of modern electronic noses (e-noses) and their most important applications in food control in this new century. The three components of an electronic nose (sample handling system, detection system, and data processing system) are described. Special attention is devoted to the promising mass spectrometry based e-noses, due to their advantages over the more classical gas sensors. Applications described include process monitoring, shelf-life investigation, freshness evaluation, authenticity assessment, as well as other general aspects of the utilization of electronic noses in food control. Finally, some interesting remarks concerning the strengths and weaknesses of electronic noses in food control are also mentioned.  相似文献
3.
中性红作为DNA作用方式光谱探针的研究   总被引:16,自引:0,他引:16  
以溴乙锭(EB)为探针在研究吩噻嗪药物与DNA间相互作用的基础上,用中性红(Neutral Red,NR)代替EB为DNA作用方式对光谱探针的可行性进行了研究。结果表明,NR作为DNA作用方式光谱探针与EB具有可比性,且由于其低毒性和高稳定性而具有较好的应用潜力。  相似文献
4.
Label-free screening of bio-molecular interactions   总被引:8,自引:0,他引:8  
The majority of techniques currently employed to interrogate a biomolecular interaction require some type of radio- or enzymatic- or fluorescent-labelling to report the binding event. However, there is an increasing awareness of novel techniques that do not require labelling of the ligand or the receptor, and that allow virtually any complex to be screened with minimal assay development. This review focuses on three major label-free screening platforms: surface plasmon resonance biosensors, acoustic biosensors, and calorimetric biosensors. Scientists in both academia and industry are using biosensors in areas that encompass almost all areas drug discovery, diagnostics, and the life sciences. The capabilities and advantages of each technique are compared and key applications involving small molecules, proteins, oligonucleotides, bacteriophage, viruses, bacteria, and cells are reviewed. The role of the interface between the biosensor surface (in the case of SPR and acoustic biosensors) and the chemical or biological systems to be studied is also covered with attention to the covalent and non-covalent coupling chemistries commonly employed.  相似文献
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表面等离子体共振法检测人血清白蛋白抗体活性   总被引:6,自引:0,他引:6  
表面等离子体共振法是研究生物大分子间相互作用的有效方法之一,和非直接方法(如酶联免疫)相比,具有实时、快速和免标记等特点。我们在甲羧基化葡聚糖修饰的传感片表面直接交联固一人血清白蛋白(HSA),用于anti-HSA抗体活性的检测,并用H3PO4(0.1mol/L)溶液再生。结果表明表面等离子体共振(SPR)生物传感器能快速实时检测anti-HSA抗体的活性,且传感片能够重复使用100次以上。  相似文献
7.
运用自行研制的表面等离子体子共振(SPR)生物传感器,采用自组装成膜技 术并以戊二醛作偶联剂,在传感片表面修饰HBsAg单克隆抗体,将其用于乙肝表面 抗原(HBsAg)的检测。实验结果表明SPR生物传感器对HBsAg的检出限为0.06ng/mL 。与传统的酶联免疫吸附试验(ELISA)相比,SPR生物传感器的检出灵敏度明显高 于ELISA法。用该SPR生物传感器对HBsAg质控血清与纯化的HBsAg溶液进行比较检测 ,结果表明该SPR生物传感器对HBsAg具有好的特异选择性。  相似文献
8.
表面等离子体共振技术研究生物膜的进展   总被引:6,自引:0,他引:6  
利用表面等离子体共振技术研究生物膜是近年来兴起的一个热门课题。文章综述了表面等离子体共振技术的基本原理、主要的成膜方式、研究进展以及发展趋势。  相似文献
9.
用双还原法制备三角形银纳米片及其光学性能   总被引:6,自引:0,他引:6       下载免费PDF全文
在硼氢化钠和柠檬酸三钠共存的体系中还原硝酸银, 以聚乙烯吡咯烷酮(PVP)为表面活性剂和保护剂, 水浴加热制备得到三角形银纳米片, 用X射线衍射(XRD)、透射电子显微镜(TEM)、紫外-可见(UV-Vis)吸收光谱、表面增强拉曼散射(SERS)光谱对其进行了表征. 结果表明: 三角形银纳米片产物为立方相金属银, 边长为(100±40) nm, 厚度为(10±5) nm; 产物表现出与球形银纳米粒子完全不同的吸收光谱; 柠檬酸根在银晶核不同晶面的选择吸附、PVP的包覆作用及Ag(111)晶面的层错对产物的形成起决定作用; 与球形纳米颗粒相比, 三角形银纳米片膜对吡啶(Py)分子有显著的SERS活性.  相似文献
10.
The intense colors of noble metal nanoparticles have inspired artists and fascinated scientists for hundreds of years. In this review, we describe refractive index sensing platforms based on the tunability of the localized surface plasmon resonance (LSPR) of arrays of silver nanoparticles and of single nanoparticles. Specifically, the color associated with single nanoparticles and surface-confined nanoparticle arrays will be shown to be tunable and useful as platforms for chemical and biological sensing. Finally, the LSPR nanosensor will be compared to traditional, flat surface, propagating surface plasmon resonance sensors.  相似文献
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