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1.
表面增强拉曼光谱研究吸附态的变化   总被引:4,自引:0,他引:4  
测量了吡啶分子在电化学处理的银表面和亚甲基蓝分子在硝酸刻蚀银表面的表面增强拉曼光谱(SERS).对SERS谱线的分析结果表明,吡啶分子在较低的表面吸附分子密度时倾向于平躺方式;而在较高的表面分子密度时,则倾向于向垂直吸附态转化.亚甲基蓝分子在较低浓度时主要是平躺吸附,随Cl-浓度的增加,亚甲基蓝从“平躺”吸附态转向“站立”吸附态.  相似文献
2.
SERS标记纳米粒子用于免疫识别   总被引:4,自引:1,他引:3       下载免费PDF全文
激光拉曼光谱技术近年来已成为研究生物分子结构常用的光谱手段.尤其在研究水溶液中蛋白质的结构和构象方面发挥了重要作用.然而,常规拉曼光谱的信号强度很低,限制了其在各个领域中的应用.表面增强拉曼光谱(SERS)和表面增强共振拉曼光谱(SERRS)技术可使信号增强6~10个数量级,尤其是SERS技术已发展到检测单分子的水平,更为其在生物方面的应用开拓了新的局.  相似文献
3.
顾仁敖  蒋芸  孙玉华 《化学学报》2004,62(23):2352-2354
利用共焦显微拉曼系统研究了在乙腈非水体系中,二甲基亚砜(姗)与乙腈的竞争吸附,同时研究了咪唑在非水乙腈体系中的吸附情况.结果表明:在非水乙腈溶液中存在着明显的竞争吸附现象,咪唑和二甲基亚砜较乙腈分子在银电极上优先吸附,明显抑制了乙腈分子的吸附,随着电位负移,咪唑或二甲基亚砜逐渐脱附,更多的乙腈分子才得以接近电极表面并开始发生解离反应.  相似文献
4.
标记免疫双组分的SERS检测研究   总被引:1,自引:0,他引:1       下载免费PDF全文
以金膜为免疫检测的基底, 采用自组装技术(Self-assembled monolayer, SAM)将ω-巯基十六酸(16-MHA)修饰于金膜后与抗体结合成固相抗体, 在此基础上组装“固相抗体-待测抗原-标记免疫金溶胶”三明治复合体系. 采用不同标记分子苯硫酚(Thiophenol)和4,4'-联吡啶(4,4'-Bipyridine)分别标记不同的免疫金溶胶, 利用表面增强拉曼光谱(SERS)谱峰较窄且具有较强的分辨率及高灵敏度的特点, 通过对两种标记分子特征谱峰的判断识别所加入的两种抗原. 通过选择合适的标记分子和一定尺度的免疫溶胶, 标记免疫SERS检测的检测限可达到飞克级(1—100 fg/mL).  相似文献
5.
表面增强拉曼光谱检测联苯胺   总被引:1,自引:0,他引:1       下载免费PDF全文
采用柠檬酸钠还原法制备了具有表面增强拉曼散射(SERS)活性的银纳米溶胶, 利用透射电子显微镜、 扫描电子显微镜和紫外-可见光谱仪对银纳米溶胶进行了表征. 对水相的联苯胺进行了SERS研究, 并对联苯胺的拉曼谱带进行了归属. 考察了团聚剂氯化镁的浓度对检测的影响, 发现随着氯化镁浓度的变大, SERS信号呈现出先增大后减弱的趋势, 即氯化镁的浓度存在一个最佳值, 此时联苯胺的检测限可达到10-8 mol/L.  相似文献
6.
SERS: a versatile tool in chemical and biochemical diagnostics   总被引:1,自引:0,他引:1  
Raman spectroscopy is a valuable tool in various research fields. The technique yields structural information from all kind of samples often without the need for extensive sample preparation. Since the Raman signals are inherently weak and therefore do not allow one to investigate substances in low concentrations, one possible approach is surface-enhanced (resonance) Raman spectroscopy. Here, rough coin metal surfaces enhance the Raman signal by a factor of 104–1015, depending on the applied method. In this review we discuss recent developments in SERS spectroscopy and their impact on different research fields.  相似文献
7.
The dye nuclear fast red has been detected and determined semi-quantitatively by means of surface enhanced resonance Raman scattering (SERRS) and surface enhanced Raman scattering (SERS), using laser exciting wavelengths of 514.5 and 632.8 nm, respectively, by employing a citrate-reduced silver colloid. A good linear correlation is observed for the dependence of the intensities of the SERRS bands at 989 cm−1 (R=0.9897) and 1278 cm−1 (R=0.9872) on dye concentration over the range 10−9 to 10−7 M, when using an exciting wavelength of 514.5 nm. At dye concentrations above 10−7 M, the concentration dependence of the SERRS signals is non-linear. This is almost certainly due to the coverage of the colloidal silver particles being in excess of a full monolayer of the dye. A linear correlation is also observed for the dependence of the intensities of the SERS bands at 989 cm−1 (R=0.9739) and 1278 cm−1 (R=0.9838) on the dye concentration over the range 10−8 to 10−6 M when using an exciting wavelength of 632.8 nm. Strong fluorescence prevented collection of resonance Raman scattering (RRS) spectra from powdered samples or aqueous solutions of the dye using an exciting wavelength of 514.5 nm, but weak bands were observed in the spectra obtained from both powdered and aqueous samples of the dye using an exciting wavelength of 632.8 nm. A study of the pH dependence of SERRS/SERS and UV–VIS absorption spectra revealed the presence of different ionisation states of the dye. The limits of detection for nuclear fast red by SERRS (514.5 nm), SERS (632.8 nm) and visible spectroscopy (535 nm) are 9, 89 and 1000 ng ml−1, respectively.  相似文献
8.
SERS technique was used to study the chemisorption kinetics of Methylene Blue (MB) on the HNO3-etched silver surface. The adsorption kinetic parameters were deduced from different vibrational modes at a low concentration of 3.5×10-6 mol/L, and it showed that MB adsorbed uniformly (monolayerly) on silver surface. However, the adsorptive behavior turned anomalous at relatively higher concentrations and a possible explanation was suggested. In addition, the influence of Cl- ions on the adsorption states of MB was investigated, and it was shown that MB molecules, adsorbed on the silver surface, tended to transform from the "lying-down" state to the "end- on"4 state after Cl- ions were added.  相似文献
9.
Wu ZS  Zhou GZ  Jiang JH  Shen GL  Yu RQ 《Talanta》2006,70(3):533-539
It is difficult to detect glucose by surface-enhanced Raman spectroscopy (SERS) due to the small normal Raman cross-section and the weak adsorption of glucose molecules on the surface of noble metal. A simple and fast method is proposed in this paper for the detection of glucose based on SERS signal of the enzyme reaction product and the difficulties have been circumvented. Gold colloids modified by horseradish peroxidase and glucose oxidase (HRP/GOD-gold colloids) are added to the mixture of o-phenylenediamine and glucose, and the resulting solution is allowed to react at room temperature for 5 min. Azoaniline, an azo compound with strong Raman scattering, is generated and the Raman scattering of this reaction product is enhanced when adsorbed on gold colloids. The intensity of the SERS spectrum is used for assessment of glucose content. The dynamic signal range provided by this analytical system is 0.50-32 mM, which covers the normal clinical range for glucose in blood from 3.5 to 6.1 mM. The detection limit is about 0.46 mM. The interference effect of several proteins on glucose detection is also investigated and has shown to have no effect on the measurement of glucose by the described technique.  相似文献
10.
Surface-enhanced Raman spectroscopy is used for in situ study of an iron surface in aqueous carbonate solutions both on open circuit and at constant potentials. Raman signals are assigned to FeCO3 and adsorbed water. Formation of FeCO3 corresponds to lowering of iron dissolution.  相似文献
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