超级微波消解－石墨炉原子吸收光谱法测定香菇中的镉

为建立一种快速批量检测香菇中镉的方法,建立了超级微波消解－石墨炉原子吸收光谱法测定香菇中镉含量的方法。分别采用湿法消解、普通微波消解和超级微波、传统微波和湿法消解技术前处理香菇标准物质样品,对比消解效果,优化超级微波消解技术的酸体系和消解最终温度。;采用石墨炉原子吸收光谱仪法测定,优化基体改进剂、灰化温度等工作参数,确定仪器最佳检测条件,验证分析方法的准确性和稳定性。结果表明,超级微波消解优于湿法消解和传统微波消解技术,准确性和重复性最佳,大幅减少用酸量的同时更加安全、高效,避免样品污染。在最优分析方法条件下,镉在0~4.0 μg/L范围内所得回归方程线性关系良好,相关系数(R)为0.9994,方法检出限为0.001 mg/kg,精密度RSD为1.4%~2.5%。超级微波消解－石墨炉原子吸收光谱法检测的探索与应用超级微波消解优于湿法消解和传统微波消解技术,大幅减少用酸量的同时更加安全、高效,避免样品污染,为农产品镉含量批量检测提供可靠的方法支撑。     

Proteome Analysis and In Vitro Antiviral,Anticancer and Antioxidant Capacities of the Aqueous Extracts of Lentinula edodes and Pleurotus ostreatus Edible Mushrooms

In this study, we examined aqueous extracts of the edible mushrooms Pleurotus ostreatus (oyster mushroom) and Lentinula edodes (shiitake mushroom). Proteome analysis was conducted using LC-Triple TOF-MS and showed the expression of 753 proteins by Pleurotus ostreatus, and 432 proteins by Lentinula edodes. Bioactive peptides: Rab GDP dissociation inhibitor, superoxide dismutase, thioredoxin reductase, serine proteinase and lectin, were identified in both mushrooms. The extracts also included promising bioactive compounds including phenolics, flavonoids, vitamins and amino acids. The extracts showed promising antiviral activities, with a selectivity index (SI) of 4.5 for Pleurotus ostreatus against adenovirus (Ad7), and a slight activity for Lentinula edodes against herpes simplex-II (HSV-2). The extracts were not cytotoxic to normal human peripheral blood mononuclear cells (PBMCs). On the contrary, they showed moderate cytotoxicity against various cancer cell lines. Additionally, antioxidant activity was assessed using DPPH radical scavenging, ABTS radical cation scavenging and ORAC assays. The two extracts showed potential antioxidant activities, with the maximum activity seen for Pleurotus ostreatus (IC50 µg/mL) = 39.46 ± 1.27 for DPPH; 11.22 ± 1.81 for ABTS; and 21.40 ± 2.20 for ORAC assays. This study encourages the use of these mushrooms in medicine in the light of their low cytotoxicity on normal PBMCs vis à vis their antiviral, antitumor and antioxidant capabilities.