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建立了同时测定牙膏和漱口水中23种致癌染料的高效液相色谱(HPLC)检测方法。漱口水样品直接以乙醇溶解稀释,牙膏样品烘干后以乙醇超声提取。采用ZORBAX Eclipse XDB-Phenyl(150 mm×4.6 mm×5μm)色谱柱进行分离,以2.5 mmol/L磷酸二氢四丁基铵水溶液(pH 7.5)和甲醇-乙腈(体积比50∶50)为流动相进行梯度洗脱,二极管阵列检测器(DAD)在400、500、600 nm波长下检测,外标法定量。结果表明,23种致癌染料在32 min内完成色谱分离,在相应的质量浓度范围内线性良好,相关系数(r2)均不小于0.9991,检出限(LOD)和定量下限(LOQ)分别为0.06~0.66 mg/L和0.18~1.98 mg/L。以阴性牙膏和漱口水为样品基质,23种致癌染料在3个加标水平下的平均回收率分别为91.2%~104%和92.0%~103%,相对标准偏差(RSD,n=6)分别为2.5%~5.8%和2.3%~5.5%。对239个牙膏和88个漱口水样品进行检测,1个牙膏样品中检出碱性紫3。该方法灵敏度高,精密度好,准确度高,适用于牙膏和漱口水中致癌染料的测定,可为牙膏和漱口水的质量安全监控提供参考。  相似文献   
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Background: DNA-RNA compounds have shown promising protection against cell oxidative stress. This study aimed to assess the cytotoxicity, protective, or preventive effect of different experimental formulations on oral epithelia’s oxidative stress in vitro. Methods: Reconstituted human oral epithelia (RHOE) were grown air-lifted in a continuous-flow bioreactor. Mouthwashes and gels containing DNA-RNA compounds and other bioactive molecules were tested on a model of oxidative stress generated by hydrogen peroxide treatment. Epithelia viability was evaluated using a biochemical MTT-based assay and confocal microscopy; structural and ultrastructural morphology was evaluated by light microscopy and TEM. Results: DNA-RNA showed non-cytotoxic activity and effectively protected against oxidative stress, but did not help in its prevention. Gel formulations did not express adequate activity compared to the mouthwashes. Excipients played a fundamental role in enhancing or even decreasing the bioactive molecules’ effect. Conclusion: A mouthwash formulation with hydrolyzed DNA-RNA effectively protected against oxidative stress without additional enhancement by other bioactive molecules. Active compounds, such as hyaluronic acid, β-Glucan, allantoin, bisabolol, ruscogenin, and essential oils, showed a protective effect against oxidative stress, which was not synergistic with the one of DNA-RNA. Incorporation of surfactant agents showed a reduced, yet significant, cytotoxic effect.  相似文献   
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