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离子色谱荧光检测法测定废水中痕量2-氨基联苯和4-氨基联苯 总被引:2,自引:0,他引:2
采用离子色谱荧光检测法测定废水中的2-氨基联苯(2-ADP)和4-氨基联苯(4-ADP),流动相为1.0mL/min的0.06mol/L NaCl,0.08mol/L HCl,40%(V/V)ACN,分离柱为Dinoex OmniPac PCX-500,该法具有良好的重现性和线性关系,2-ADP和4-ADP的回收率分别为98.5%-101.4%和102.2%-104.0%,检测限分别为0.006mg/L和0.10mg/L。 相似文献
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小肠蔗糖酶的化学修饰及对酶活力的影响 总被引:3,自引:0,他引:3
考察了苯甲基磺酰氟、N-溴化琥珀酰亚胺、琥珀酸酐、三硝基苯磺酸对猪小肠蔗糖酶活性的影响。将猪小肠匀浆后的粗提取物进行分段盐析、DEAB-Sepharose柱层析及Sephacryl S-200、Sephadex G-200凝胶过滤等纯化步骤,得到聚丙烯酰胺凝胶电泳为单一蛋白质区带,比活力为1.79units/mg,经12%SDS-聚丙烯酰胺凝胶电泳为一条区带,其亚基相对分子质量为51000。该酶的功能基团是半胱氨酸的巯基,蔗糖酶分子内没有二硫键存在。 相似文献
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建立了一种利用阴离子交换色谱柱分离,抑制型电导检测器同时测定乳酸、丙酸、丙酮酸、苹果酸、磷酸二羟丙酮、柠檬酸、异柠檬酸7种有机酸和单磷酸核苷酸(AMP)、双磷酸核苷酸(ADP)、三磷酸核苷酸(ATP)3种核苷酸的方法。采用IonPacAS11-HC阴离子交换柱,KOH梯度洗脱,流速1.00mL/min,柱温30℃,进样体积25μL。方法的相对标准偏差为0.39%~4.69%,线性相关系数为0.9906~0.9996,加标回收率为80.71%~94.02%,方法可用于嗜热厌氧菌胞外培养液中有机酸和核苷酸的同时测定。 相似文献
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Sankaran Chandramouleeswaran Bhavaniamma Vijayalakshmi Satrugnan Kartihkeyan Talasila Prasada Rao Chonatumatom S. P. Iyer 《Mikrochimica acta》1998,128(1-2):75-77
A highly sensitive and selective ion-chromatographic procedure with UV detection at 227 nm was developed for the determination of trace amounts of iodide. The procedure utilizes 0.1M NaOH as eluent, which enables the determination of 1–100 g I–1 I– with a precision of ca. 6%. 1000-fold amounts of other anions such as fluoride, chloride, phosphate, sulphate, bromide, nitrite and nitrate do not interfere. The method has been applied to determine iodide in sea water samples from the Indian coast. 相似文献
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G. Brandt A. Kettrup 《International journal of environmental analytical chemistry》2013,93(2-4):129-143
Abstract In recent years methods have been developed to determine organic halogen at the μg/1 level in water samples by adsorbing these compounds on active carbon and by detecting the inorganic halides formed after conversion of the adsorbates by pyrohydrolysis. Applying these techniques the analysis of the so-called group parameter “Adsorbable Organic Halogen (AOX)” is performed. The distinction of each of the halogens in the group parameter AOX and the determination of the parameter “adsorbable organic sulfur compounds (AOS)” can be realized using ion-chromatography for the detection of the anions, obtained after pyrohydrolysis of the adsorbed organic compounds. Further investigations have shown good adsorption capacity of a newly developed nearly chlorine- and sulfur-free active carbon for organic model substances. This report presents the examinations concerning pyrohydrolysis of the organic solutes and absorption of the formed inorganic species. The conditions for complete conversion of the model substances and high recovery rates in inorganic anions have been proved successfully. The optimization of the pyrohydrolysis apparatus and of the combustion conditions have been performed and proved with good results. 相似文献
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