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1.
酶直接电化学与第三代生物传感器   总被引:10,自引:0,他引:10  
池其金  董绍俊 《分析化学》1994,22(10):1065-1072
本文详细地评述并展望了酶直接电化学与第三代生物传感器这个领域已取得的成果,主要内容涉及生物电催化的三个发展阶段,实现酶与电极之间的直接电子转移方法和相应机理、以及第三代酶传感器的研制。  相似文献
2.
辣根过氧化物酶/聚邻苯二胺膜电极的制备与性能研究   总被引:5,自引:0,他引:5  
魏东  吴辉煌 《电化学》1995,1(2):186-192
辣根过氧化物酶(HRP)/聚邻苯二胺(PPD)膜电极由pH7.0磷酸盐缓冲溶液介质中邻苯二胺在玻碳电极上的电聚合而制得。讨论了HRP电化学固定化的过程。所得酶电极呈现生物催化活性,可以没有电子传递体存在的情况下催化H2O2还原。该反应发生在聚邻苯二胺氧化还原的电位区,聚合物参与了酶的电子转移过程。分析了旋转HRP/PPD电极上酶反应的动力学,讨论了动力学常数的影响因素。  相似文献
3.
聚邻苯二胺膜电极中辣根过氧化物酶的电子传递   总被引:3,自引:0,他引:3  
利用酸的电化学固定法制备含辣根过氧化物酶的聚邻苯二胺膜电极,研究其伏安行为及对H2O2还原的生物电催化作用,结果表明,在所述生物电催化反应中酶与聚合物基质 直接电子传递,但对新制的酶电极而言,电聚合时生成并包埋在酶膜中的寡聚体可作为电子传递体加速氧化态酶的再生,根据酶电极电流响应实验曲线的拟合,发现经态酶的再生速度随是极电位的变化表观上符合Tafel关系式,提出了酶反应学参数的测定方法。  相似文献
4.
维生素B12及其衍生物的电化学研究   总被引:2,自引:0,他引:2  
评述了维生素B12及其衍生物的电化学研究,讨论了电化学在理解B12生理功能方面的重要性,阐述了有关B12电化学研究的最新进展,预测了研究的前景。  相似文献
5.
The direct electrochemistry of redox enzymes (or proteins) has received more and more attention[1—9]. These studies developed an electrochemical basis for the investigation of enzyme structure, mechanisms of redox transformations of enzyme molecules and metabolic processes involving redox transformations. From these studies, one can also find potential appli-cations of enzymes in biotechnology. For example, if an enzyme immobilized on electrode surface is ca-pable of the direct electron tra…  相似文献
6.
葡萄糖氧化酶在活性炭上的固定及直接电化学   总被引:2,自引:0,他引:2       下载免费PDF全文
The glucose oxidase (GOD) immobilized onto the surface of activated carbon powders at the glassy carbon electrode (GOD-C/GC) could undergo the quasi-reversible, direct electrochemical reaction. Its formal redox potential, E0′, is almost independent on the scan rates. The average value of E0′ is (-0.467 ± 0.002) V (vs SCE) in the pH 6.8 phosphate buffer solution. Its apparent heterogeneous electron transfer rate constant (ks) is (1.18 ± 0.59) s-1, which is much higher than that reported previously. The dependence of E0′ on the pH of the buffer solution indicated that the direct electrochemical reaction of the immobilized GOD is a two-electron transfer reaction process coupled with two-proton transfer. The further experimental results demonstrated that the immobilized GOD retained its bioelectrocatalytic activity to the oxidation of β-D(+) glucose.  相似文献
7.
以聚芳酰胺-多壁碳纳米管混合物为载体, 利用漆酶表面氨基与聚芳酰胺主链端羧基的共价偶联以及碳纳米管与漆酶间的疏水作用, 构筑了具有较高稳定性和电催化活性的漆酶修饰电极. 并对该固酶修饰电极的固酶量、酶活力、电化学行为及其电催化氧还原的性能进行了表征. 对漆酶分子具有亲和力的聚芳酰胺芳环结构及聚芳酰胺端羧基与漆酶表面氨基的共价偶联避免了漆酶的脱落和变性. 而碳纳米管与聚芳酰胺的混合使得该三维修饰电极具有良好的电子导电性, 并成功地实现了漆酶的氧化还原活性位与电极之间的直接电荷转移, 这一点可由在0.73 和0.38 V附近观察到漆酶的T1和T2 (漆酶的T1, T2铜活性位的形式电位分别为0.78 和0.39 V (vs NHE))铜活性位的两对氧化还原峰确认. 漆酶的担载量为56.0 mg·g-1, 具有电化学活性的漆酶占总担载漆酶量的68%. 在pH=4.4磷酸盐缓冲溶液中, 该修饰电极上氧气还原的起始电位为0.55 V, 其对氧气的米氏常数KM为55.8 μmol·L-1, 对氧气的检测限为0.57 μmol·L-1. 在4 ℃下保存两个月后能实现直接电荷转移的漆酶量仅下降了14%左右而氧还原超电势提高了约50 mV. 结果表明该修饰电极有望用作酶基生物燃料电池的阴极和电流型氧气传感器.  相似文献
8.
将肌红蛋白(Mb)通过吸附的方法固定在碳纳米管(CNT)表面, 用AFM、XPS、UV-Vis和FTIR对其进行了表征, 研究了CNT对Mb直接电子转移反应的促进作用. 循环伏安结果表明, Mb在CNT表面能进行有效和稳定的直接电子转移反应, 其循环伏安曲线上表现出一对良好的、几乎对称的氧化还原峰; 在20−160 mV•s−1的扫速范围内, 式量电位E0′几乎不随扫速而变化, 其平均值为(−0.343±0.001) V (vs SCE, pH 7.0); Mb在CNT表面直接电子转移的表观速率常数为(3.11±0.98) s−1; 式量电位E0′与溶液pH的关系表明, Mb的直接电化学过程是一个有H+参与的电极过程. 进一步的实验结果显示, 固定在CNT表面的Mb能保持其对H2O2和O2还原的生物电催化活性.  相似文献
9.
Catalytic properties of monoamino oxidase (MAO) in a homogeneous reaction of oxidation and a heterogeneous reaction of bioelectrocatalytic oxidation of benzylamine by derivatives of phenothiazine and phenoxazine (artificial electron acceptors) is studied. The efficiency of electroenzymic catalysis involving Methylene Blue and MAO immobilized on the electrode is comparable to that of homogeneous enzymic catalysis. When immobilized in a film of polymer electrolyte Eastman AQ-29, Methylene Blue and Toluidine Blue efficiently carry electrons from the reduced redox center of immobilized MAO onto a glassy-carbon electrode. Dependence of the oxidation current of these compounds on the concentration of the benzylamine substrate is studied and it is shown that the enzymic reaction is the limiting stage in a bioelectrocatalytic process. This conclusion gives grounds for using the dependence of the anodic current on the monoamine concentration as a calibration plot when assaying biogenic amines.  相似文献
10.
Structure and Properties of Polymer Biocomposite Materials   总被引:1,自引:0,他引:1  
Results of studying the structure and properties of biocomposite materials are summarized. The materials in question include an enzyme (laccase, peroxidase), an ion- or electron-conducting polymer (Nafion, polymethylpyrrole), and a carbon substrate (compact, disperse). It is shown that the orientation of a large number of enzyme molecules in an enzyme/Nafion composite material on the substrate surface favors direct bioelectrocatalysis. During co-immobilization of an enzyme and polymethylpyrrole, conditions are realized under which the polymer takes part in the electron transfer between the active center of the enzyme and the surface of the electroconducting substrate. A fresh approach to constructing a biocomposite material is developed. The material is based on an extremely finely divided carbon material (colloidal graphite), which ensures a high specific activity of laccase immobilized on it. The size of colloidal-graphite particles is commensurate with that of the laccase molecule, owing to which the enzyme macromolecule is surrounded by carbon particles. As a result, practically all adsorbed enzyme molecules are electrochemically active and participate in direct bioelectrocatalysis.  相似文献
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