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Poyye Dsouza Priya Swetha Jospeh Sonia Kannan Sapna K. Sudhakara Prasad 《Current Opinion in Electrochemistry》2021
Even though global health has been steadily improved, the global disease burden associated with communicable and non-communicable diseases extensively increased healthcare expenditure. The present COVID-19 pandemic scenario has again ascertained the importance of clinical diagnostics as a basis to make life-saving decisions. In this context, there is a need for developing next-generation integrated smart real-time responsive biosensors with high selectivity and sensitivity. The emergence of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas biosensing systems has shown remarkable potential for developing next-generation biosensors. CRISPR/Cas integrated electrochemical biosensors (E-CRISPR) stands out with excellent properties. In this opinionated review, we illustrate the rapidly evolving applications for E-CRISPR-integrated detection systems towards biosensing and the future scope associated with E-CRISPR based diagnostics. 相似文献
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Thermo‐triggered Release of CRISPR‐Cas9 System by Lipid‐Encapsulated Gold Nanoparticles for Tumor Therapy 下载免费PDF全文
Dr. Peng Wang Dr. Lingmin Zhang Prof. Wenfu Zheng Dr. Liman Cong Zhaorong Guo Yangzhouyun Xie Le Wang Rongbing Tang Qiang Feng Prof. Yoh Hamada Prof. Kohsuke Gonda Prof. Zhijian Hu Prof. Xiaochun Wu Prof. Xingyu Jiang 《Angewandte Chemie (International ed. in English)》2018,57(6):1491-1496
CRISPR/Cas9 system is a powerful toolbox for gene editing. However, the low delivery efficiency is still a big hurdle impeding its applications. Herein, we report a strategy to deliver Cas9‐sgPlk‐1 plasmids (CP) by a multifunctional vehicle for tumor therapy. We condensed CPs on TAT peptide‐modified Au nanoparticles (AuNPs/CP, ACP) via electrostatic interactions, and coated lipids (DOTAP, DOPE, cholesterol, PEG2000‐DSPE) on the ACP to form lipid‐encapsulated, AuNPs‐condensed CP (LACP). LACP can enter tumor cells and release CP into the cytosol by laser‐triggered thermo‐effects of the AuNPs; the CP can enter nuclei by TAT guidance, enabling effective knock‐outs of target gene (Plk‐1) of tumor (melanoma) and inhibition of the tumor both in vitro and in vivo. This AuNPs‐condensed, lipid‐encapsulated, and laser‐controlled delivery system provides a versatile method for high efficiency CRISPR/Cas9 delivery and targeted gene editing for treatment of a wide spectrum of diseases. 相似文献
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Yan Lyu Shasha He Jingchao Li Yuyan Jiang He Sun Yansong Miao Kanyi Pu 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(50):18365-18369
Noninvasive regulation of CRISPR/Cas9 gene editing is conducive to understanding of gene function and development of gene therapy; however, it remains challenging. Herein, a photolabile semiconducting polymer nanotransducer (pSPN) is synthesized to act as the gene vector to deliver CRISPR/Cas9 plasmids into cells and also as the photoregulator to remotely activate gene editing. pSPN comprises a 1O2‐generating backbone grafted with polyethylenimine brushes through 1O2‐cleavable linkers. NIR photoirradiation spontaneously triggers the cleavage of gene vectors from pSPN, resulting in the release of CRISPR/Cas9 plasmids and subsequently initiating gene editing. This system affords 15‐ and 1.8‐fold enhancement in repaired gene expression relative to the nonirradiated controls in living cells and mice, respectively. As this approach does not require any specific modifications on biomolecular components, pSPN represents the first generic nanotransducer for in vivo regulation of CRISPR/Cas9 gene editing. 相似文献
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Inside Cover: Active Intracellular Delivery of a Cas9/sgRNA Complex Using Ultrasound‐Propelled Nanomotors (Angew. Chem. Int. Ed. 10/2018) 下载免费PDF全文
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CRISPR(Clustered regularly interspaced short palindromic repeats)技术是一种革命性的基因编辑和调控工具,问世之后迅速成为了生物医学领域的前沿热点,广泛用于基因功能研究和治疗。CRISPR具有优异的序列识别性质,核酸切割能力,并且易于编程设计改造,在生物分析化学领域展示出独特的魅力,在病毒检测、临床诊断和单细胞成像分析等方面都取得了突破性进展。目前基于CRISPR技术的检测方法种类繁多,本文综述了CRISPR-Cas分析检测方法的研究进展,并且展望了该技术的发展趋势。 相似文献
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Active Intracellular Delivery of a Cas9/sgRNA Complex Using Ultrasound‐Propelled Nanomotors 下载免费PDF全文
Malthe Hansen‐Bruhn Dr. Berta Esteban‐Fernández de Ávila Dr. Mara Beltrán‐Gastélum Prof. Jing Zhao Dr. Doris E. Ramírez‐Herrera Pavimol Angsantikul Prof. Kurt Vesterager Gothelf Prof. Liangfang Zhang Prof. Joseph Wang 《Angewandte Chemie (International ed. in English)》2018,57(10):2657-2661
Direct and rapid intracellular delivery of a functional Cas9/sgRNA complex using ultrasound‐powered nanomotors is reported. The Cas9/sgRNA complex is loaded onto the nanomotor surface through a reversible disulfide linkage. A 5 min ultrasound treatment enables the Cas9/sgRNA‐loaded nanomotors to directly penetrate through the plasma membrane of GFP‐expressing B16F10 cells. The Cas9/sgRNA is released inside the cells to achieve highly effective GFP gene knockout. The acoustic Cas9/sgRNA‐loaded nanomotors display more than 80 % GFP knockout within 2 h of cell incubation compared to 30 % knockout using static nanowires. More impressively, the nanomotors enable highly efficient knockout with just 0.6 nm of the Cas9/sgRNA complex. This nanomotor‐based intracellular delivery method thus offers an attractive route to overcome physiological barriers for intracellular delivery of functional proteins and RNAs, thus indicating considerable promise for highly efficient therapeutic applications. 相似文献
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Yu Zhang Xinyu Ling Xiaoxuan Su Shilin Zhang Dr. Jing Wang Dr. Pingjing Zhang Dr. Wenjian Feng Dr. York Yuanyuan Zhu Prof. Dr. Tao Liu Prof. Dr. Xinjing Tang 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2020,132(47):21081-21085
Currently CRISPR/Cas9 is a widely used efficient tool for gene editing. Precise control over the CRISPR/Cas9 system with high temporal and spatial resolution is essential for studying gene regulation and editing. Here, we synthesized a novel light-controlled crRNA by coupling vitamin E and a photolabile linker at the 5′ terminus to inactivate the CRISPR/Cas9 system. The vitamin E modification did not affect ribonucleoprotein (RNP) formation of Cas9/crRNA/tracrRNA complexes but did inhibit the association of RNP with the target DNA. Upon light irradiation, vitamin E-caged crRNA was successfully activated to achieve light-induced genome editing of vascular endothelial cell-growth factor A (VEGFA) in human cells through a T7E1 assay and Sanger sequencing as well as gene knockdown of EGFP expression in EGFP stably expressing cells. This new caging strategy for crRNA could provide new methods for spatiotemporal photoregulation of CRISPR/Cas9-mediated gene editing. 相似文献
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CRISPR/Cas是大多数细菌及古细菌基于RNA的后天免疫系统。由CRISPR/Cas系统改造而成的CRISPR/Cas技术已成为一种强大的基因编辑工具,广泛应用于基因功能研究和基因修饰与治疗。除了作为基因编辑工具,Ⅱ类Cas蛋白具有的"附属切割"特性,已被开发成一种快速、低成本且高灵敏的核酸检测工具,在核酸分子诊断领域具有重要的应用潜力。该文总结了3种Ⅱ类Cas蛋白在核酸检测领域的代表性研究进展,并对CRISPR/Cas系统在该领域的应用前景进行了展望。 相似文献