全文获取类型
收费全文 | 811篇 |
免费 | 188篇 |
国内免费 | 72篇 |
专业分类
化学 | 402篇 |
晶体学 | 11篇 |
力学 | 88篇 |
综合类 | 91篇 |
数学 | 141篇 |
物理学 | 338篇 |
出版年
2024年 | 1篇 |
2023年 | 8篇 |
2022年 | 1篇 |
2021年 | 2篇 |
2020年 | 2篇 |
2019年 | 12篇 |
2018年 | 26篇 |
2017年 | 5篇 |
2016年 | 13篇 |
2015年 | 10篇 |
2014年 | 13篇 |
2013年 | 10篇 |
2012年 | 11篇 |
2011年 | 9篇 |
2010年 | 15篇 |
2009年 | 9篇 |
2008年 | 4篇 |
2007年 | 12篇 |
2006年 | 9篇 |
2005年 | 7篇 |
2004年 | 29篇 |
2003年 | 171篇 |
2002年 | 262篇 |
2001年 | 231篇 |
2000年 | 93篇 |
1999年 | 66篇 |
1998年 | 9篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 5篇 |
1994年 | 5篇 |
1993年 | 4篇 |
1992年 | 6篇 |
1991年 | 3篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1986年 | 1篇 |
1984年 | 1篇 |
排序方式: 共有1071条查询结果,搜索用时 15 毫秒
1.
合适比例增敏剂会对超级微波消解-电感耦合等离子体质谱法(ICP-MS)测定食品中硒元素含量具有一定的优化效果。本文采用超级微波消解仪对1.0 g食品样品进行全消解,采用碰撞池模式规避质谱干扰,内标校正非质谱干扰,选择甲醇、乙醇、乙酸、异丙醇、丙三醇作为增敏剂,并调整测试过程中的添加比例,用电感耦合等离子体质谱法测定。结果表明:2%异丙醇存在时硒元素各同位素响应值最高,其中78Se具有低至0.0003 mg·kg-1的方法检出限;用以上方法对食品标准物质(GSB-8、GSB-9、GSB-24、GSB-30)及实际样品进行分析,标准物质测定值均在其认定值及不确定度范围内,相对误差小于-5.4%;实际样品测定值的相对标准偏差(n=7,RSD)为0.3~1.7%,加标回收率为99.2~105%。本方法操作简便,精密度好,准确度高,可以作为各类食品样品中硒含量测试方法。 相似文献
2.
Study of the matrix effects of tetrodotoxin and its content in cooked seafood by liquid chromatography with triple quadrupole mass spectrometry 下载免费PDF全文
Xin‐wei Yu Meiling Lu Bai‐fen Huang Yi‐ping Ren 《Journal of separation science》2015,38(19):3374-3382
Tetrodotoxin is a marine biotoxin with high acute toxicity. The levels in cooked seafood will help us to assess its intake in humans and may help assess the risk of toxicity. However, heavy matrices hinder the direct quantitation of tetrodotoxin. A quantitative method of measuring tetrodotoxin in cooked seafood using liquid chromatography with triple quadrupole mass spectrometry was established in this study. Tetrodotoxin was extracted from the sample matrix using 2% formic acid in methanol and cleaned using a cation exchange cartridge. The cleanup conditions were optimized. The matrix effects were determined using the postextraction spiking method and by comparing the slope of the linear regression equation in sample matrix to that in solvent. The limit of detection in the sample matrix was 5 μg/kg and the limit of quantification was 10 μg/kg. The mean recoveries at three spiking levels were 66.9–89.2% with relative standard deviations of 5.0–10.8% (n = 6) in five different matrices. Tetrodotoxin was found at concentrations of 26.1–2462 μg/kg in nine of 83 cooked seafoods tested in this study. Eight analogs of Tetrodotoxin were detected in the samples studied. 相似文献
3.
抑制系统是抑制型(双柱型)离子色谱系统极为重要的一个组成部分,也是离子色谱有别于其它类型的液相色谱的最重要特点之一。抑制器的发展经历了多个发展时期,而目前商品化的离子色谱仪亦分别采用不同形式的抑制手段。近年来,还发展了一些特殊的辅助抑制器,如接在抑制器后面CO2除去装置,用于提高被测离子的信号或进一步降低背景电导值。 相似文献
4.
5.
6.
无机类富勒烯过渡金属硫化物纳米材料的合成及其摩擦学应用研究进展 总被引:7,自引:0,他引:7
评述了无机类富勒烯(IF)与过渡金属硫化物(WS2和MoS2等)纳米材料的合成技术及其摩擦学应用研究的最新进展,外型为球形或近似于球形并具有嵌套中空结构的IF-WS2和IF-MoS2纳米果粒具有潜在的摩擦学应用前景;对含有IF-WS2纳米颗粒的复合材料和Ni-P复合镀层的研究表明,其比含有石墨和层状2H-WS2粉末的固体润滑剂具有更优异的摩擦学性能。 相似文献
7.
8.
Rong Wang Hua Xie Yue‐bing Xu Zheng‐ping Jia Xian‐dong Meng Juan‐hong Zhang Jun Ma Juan Wang Xian‐hua Wang 《Biomedical chromatography : BMC》2012,26(3):393-399
The DNA fragment detection focusing technique has further enhanced the sensitivity and information of DNA targets. The DNA fragment detection method was established by capillary electrophoresis with laser‐induced fluorescence detection and restriction endonuclease chromatographic fingerprinting (CE‐LIF‐REF) in our experiment. The silica capillary column was coated with short linear polyarclarylamide (SLPA) using nongel sieving technology. The excision product of various restricted enzymes of DNA fragments was obtained by REF with the molecular biology software Primer Premier 5. The PBR322/BsuRI DNA marker was used to establish the optimization method. The markers were focused electrophoretically and detected by CE‐LIF. The results demonstrate that the CE‐LIF‐REF with SLPA can improve separation, sensitivity and speed of analysis. This technique may be applied to analysis of the excision product of various restricted enzymes of prokaryotic plasmid (pIRES2), eukaryote plasmid (pcDNA3.1) and the PCR product of codon 248 region of gastric cancer tissue. The results suggest that this method could very sensitively separate the excision products of various restricted enzymes at a much better resolution than the traditional agarose electrophoresis. Copyright © 2011 John Wiley & Sons, Ltd. 相似文献
9.
β‐Ionone was oxidized with O2 under solvent‐free conditions catalyzed by an N‐hydroxyphthalimide/Co(acac)2 system in mild conditions with high conversion and excellent selectivity to oxo‐β‐ionone or 5,6‐epoxy‐β‐ionone in different reaction conditions, respectively. 相似文献
10.
基于增量段上的应变关于塑性拉氏乘子的变化率为常矢量的假定,导出一种精确有效的、对有限元分析中线性混合硬化弹塑性本构方程的积分算法。以精确算例和等误差分布图的形式检验了本文方法以及其他两种常用方法的精度。 相似文献