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A γ-hydroxyphosphonate P6 (O1-methyl-O2-(1, 2, 2-trimethylpropyl)-2-hydroxy-5-nitro-phenyl methylphosphonic acid) which is proposed to be an analog of the transition state in hydrolysis of soman was synthesized. Artificial antigens were obtained by conjugating P6 to the carrier proteins BSA (bovine serum albumin) and LPH (Limulus polyphenus hemocyanin). Mice were immunized with P6-LPH and recombinant single-chain antibody phage display library was constructed. After 4 rounds of panning against P6-BSA and competitive inhibition enzyme immunoassay, more than 70 strains of phage antibodies capable of binding soman were obtained and 11 of them can accelerate the hydrolysis reaction of soman. One of them (EP6) was studied further. Soluble single-chain antibody was prepared and purification was performed by gel filtration and ion exchange chromatography. The kinetic experiment was carried out showing that the turnover number kcatt= 198 min-1 and the rate enhancement kcatkuncat = 122 419. When 0.16 mg · mL-1 相似文献
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通过相应的酸和亚硫酰氯反应合成了3种新的稠环芳基取代酰氯类化合物(9-蒽丙烯酰氯,9-蒽丙酰氯和1-芘丙烯酰氯)作为羟基药物及毒物超微量分析的荧光探针。 相似文献
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含哌啶结构杂环醇的立体化学研究 总被引:2,自引:0,他引:2
用分子力学和量子化学计算方法(AM1)研究了含哌啶结构杂环醇(1-4)及其酸酯类化合物的立体化学问题,结果表明,在哌啶醇(1)及醇(3)分子中,4位羟基处于哌啶环的α键位时, 的氮原子和羟基氧原子之间可能形成非经典的轨道超共轭作用,使其构型构象得到稳定。 相似文献
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三环羧酸酯(Ⅱ)在强碱LDA作用下经过烯醇中间体,由空气氧对其进行氧化,在羰基α-位引入一个过氧键,尔后用亚硫酸钠还原得到三环羟基羧酸酯(Ⅲ)。用量子化学AM1计算结果分析了Ⅲb分子中形成分子内氢键的原因及其与Ⅲa和Ⅲc之间的区别。 相似文献