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The pigmentation gene of Xenopus laevis is dominant and that of albino a~p mutant recessive. Hete-rologous haploid hybrids are produced by UV inactivation of the egg nuclei during second polar body for-mation in the mutant sperm-fertilized Xenopus eggs. During development of these hybrids, melanin ap-peared in the eye and melanophores in the skin at stages comparable to those of the wild type, but muchearlier than in the albino mutant. The number and intensity of pigment cells are interinediate betweenthe black Xenopus and albino mutant. While a number of pigment cells remain in the hybrids, thosein the albino eventually degenerate. Therefore, the development and maintenance of pigmentation in hete-rologous hybrids are contributed by Xenopus cytoplasm. Tadpole tail-tips were squashed and stained for chromosome counting. The results show thatXenopus and mutants are diploid (36 chromosomes) and heterologous haploid hybrids have 18 chromosomes. 相似文献
43.
A piezoelectric nucleic acid sensor was constructed ofr detection of tumor necrosis factor gene.Two methods were employed for immobilization of nucleic acid probe on gold electrode of piezoelectric crystal.The results show that polyethyleneimine adbesion and glutaraldehyde cross-linking method has higher sensitivity,stability and selectivity than protein A method.The solid-phase nucleic acid hybridization of oligo unclecotides and tumor necrosis factor target gene sequence were monitorde using this sensor.Tumor necrosis factor gene sequence(580bp) was detected by this nucleic acid sensor for the first time. 相似文献
44.
In this paper,large-sized sapphire (230×210 mm,27.5 kg) was grown by SAPMAC method (sapphire growth technique with micro-pulling and shoulder-expanding at the cooled center). Dislocation peculiarity in large sapphire boule (0001) basal plane was investigated by chemical etching,scanning electron microscopy and X-ray topography method. The triangular dislocation etch pit measured is 7.6×101~8.0×102 cm-2,in which relative high-density dislocations were generated at both initial and final stages of crystal growth. The analysis of single-crystal X-ray topography shows that there are no apparent sub-grain boundaries; the dislocation lines are isolated and straight. Finally,the origins of low-density dislocation in sapphire crystal are discussed by numerical analysis method. 相似文献
45.
痤疮患者血清中微量元素含量的变化 总被引:2,自引:0,他引:2
测定了52例痤疮患者血清Ca,Mg,Zn,Cu,Fe五种元素的含量。结果表明,痤疮患者血清Zn水平显著低于正常人(P<0.001),血清Ca,Mg,Cu,Fe水平则与正常人无差别(P>0.05)。部分病人口服补充锌剂后痤疮症状有所好转。提示痤疮患者应重视体内微量元素含量的变化,并做适当的补充。 相似文献
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IntroductionThegenediagnosistechnology ,alsocallednucleicacidprobetechnology ,hasbecomeanattractivemethodinthefieldsofbiochemistryandclinicalmedicine .Itisprovedtobeconvenientandsafeusingnon radioactivereagentswithelectrochemicalandopticaldetector.Espe c… 相似文献
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Chemical Constituents of Ailanthus triphysa 总被引:2,自引:0,他引:2
Two new compounds,8(14),15-isopimaradiene-2α,3α,19-triol(1),and 6α,7β-dihydroxy-17(20)-cis-5α-pregna-16-one(2),together with four known copounds,a oxygenated rare phyllocladane,phyllocladan-16α,19-diol(3),kaempferol-3-0-β-D-galactopyranosied,kaempferol-3-0-α-L-rhamnopyranoside and scopoletin, were isolated from the leaves of Ailanthus tripysa.Structures of 1-3 were elucidated on the basis of spectroscopic data as compared with related compounds. 相似文献
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With the aid of matrix theory and computer a series of data can be obtained such as the total number of localities in which r species of graptolites appears, the common number of localities in which both i and j species of graptolites appear, the total number of species of graptolites in one locality, the common number of species of graptolites found in both i and j localities, the total number of zones in which r species of graptolites appears, the common number of zones in which both i and j species appear, the total number of species of graptolites found in one zone, the common number of species of graptolites found in both i and j zones. Based on these data a common problem in biostratigraphy may be solved, that is, in the absence of the zonal graptolite which species of graptolites can be chosen to replace the zonal graptolite, or how to detect the zonal graptolite of a graptolite biozone. The result of matrix treatment not only detects the graptolite biozone but is useful to infer the paleo-biogeo 相似文献