Effects of Melatonin on Dairy Herd Improvement (DHI) of Holstein Cow with High SCS

Mastitis is a common disease in cows breeding. The milk quality will be significantly reduced with increased milk somatic cells, which often occurs in cows with mastitis. In this study, the influence of seasonal changes, age and lactation stages in the Dairy Herd Improvement (DHI) of cows was investigated. Then, the Dairy Herd Improvement (DHI) of cows with high somatic cell score (SCS) after melatonin treatment was systemically investigated. The results showed that melatonin significantly suppressed the milk somatic cell score under all of the tested conditions. The melatonin treatment also improved the milk nutritional value by reducing its fat but increasing its lactose and protein contents. The application of melatonin significantly improved the DHI. The beneficial effects of melatonin on DHI are likely attributed to the antioxidant and anti-inflammatory activities of melatonin.     

Lipoic Acid Combined with Melatonin Mitigates Oxidative Stress and Promotes Root Formation and Growth in Salt-Stressed Canola Seedlings (Brassica napus L.)

Lipoic acid (LA) and melatonin (MT) are pleiotropic molecules participating in plant stress resistance by modulating cellular biochemical changes, ion homeostasis, and antioxidant enzyme activities. However, the combined role of these two molecules in counteracting the detrimental impacts of salinity stress is still unknown. In the present study, we determined the effects of exogenous LA (0.5 µM), MT (1 µM) and their combination (LA + MT) on growth performance and biomass accumulation, photosynthetic pigments, enzymatic and non-enzymatic antioxidant activities, and ions homeostatic in canola (Brassica napus L.) seedlings under salinity stress (0, 100 mM) for 40 days. The results indicate that exogenous application of LA + MT improved the phenotypic growth (by 25 to 45%), root thickness (by 68%), number of later lateral roots (by 52%), root viability (by 44%), and root length (by 50%) under salinity stress. Moreover, total soluble protein, chlorophyll pigments, the concentration of superoxide dismutase (SOD), catalase peroxidase (CAT), and ascorbic peroxidase (ASA) increased with the presence of salt concentration into the growth media and then decreased with the addition of LA + MT to saline solution. Leaf protein contents and the degradation of photosynthetic pigments were lower when LA + MT treatments were added into NaCl media. The proline and phenol contents decreased in the exogenous application of LA + MT treatments more than individual LA or MT treatments under the salinity stress. The incorporation of LA or MT or a combination of LA + MT to saline solution decreased salinity-induced malondialdehyde and electrolyte leakage. In conclusion, the alteration of metabolic pathways, redox modulation, and ions homeostasis in plant tissues by the combined LA and MT application are helpful towards the adaptation of Brassica napus L. seedlings in a saline environment. The results of this study provide, for the first time, conclusive evidence about the protective role of exogenous LA + MT in canola seedlings under salinity stress.     

褪黑激素对家兔性器官和动情周期的作用

在家兔皮下埋植褪黑激素丸，每周一次，共７周．结果表明，褪黑激素对家兔性器官起抑制作用：公兔睾丸重量减轻，分泌的睾酮减少；母兔卵巢变小，血浆孕酮水平降低，卵巢发育不良，母兔无发情表现     

Determination of Melatonin in the Mian Erkang Capsules by HPLC

IntroductionTheMianErkangmadebyusisahighpotencycalciumpreparation.ItfindswideapplicationinmanysymPtomswherethereisalackofcalcium.Atthesametime,itcanhypnotizeandactasananti-decrepitude.Melatoninisoneofthemaineffectivecomponents.ItschendcalnameisN-acetyl-5-methoxytryptamine,andcanhypnotize,calm,relievepain',increasethebody'sinununity,beusedincancertherapyanddelaydecrepitude"'.ForqualitycontrolofMianErkangcapsules,arapid,sensitiveandaccurateHPLCmethodforthedeterminationofmelatonininMianErka…     

褪黑激素的合成

以5－甲氧基吲哚为起始原料，经4步反应合成了褪黑激素（MLT).5－甲氧基吲哚和草酰氯在0℃-5℃定量反应生成5－甲氧基吲哚－3－草酰氯（I），I直接用氨水胺化得98％的5－甲氧基吲哚－3－草酰胺（Ⅱ），Ⅱ用氢化铝锂还原得到5－甲氧基色胺（Ⅲ），收率57％，Ⅲ经乙酰化即得目标产物MLT，收率80％。     

褪黑素清除活性氧作用的研究

采用多种分析体系检测了褪黑素清除活性氧的作用，结果表明，褪黑素能有效地清除包括２、Ｏ２、Ｈ２Ｏ２在内的多种活性氧，能显著地抑制ＯＨ对ＤＮＡ的损伤，是一种有效的抗氧化剂。     

氧化异阿朴菲-褪黑素杂合体的合成、抗β淀粉样蛋白聚集及抗氧化活性

基于多靶向策略设计合成了氧化异阿朴菲-褪黑素杂合化合物,测试了它们的抗胆碱酯酶性能及相应的抑制动力学、抗氧化能力和抑制乙酰胆碱酯酶诱导的β-淀粉样蛋白(Aβ)聚集能力。实验结果表明,所合成的化合物对乙酰胆碱酯酶具有中等强度抑制力,其抑制IC50值在微摩尔浓度水平,属于非竞争性抑制剂;对乙酰胆碱酯酶诱导的Aβ淀粉样蛋白聚集的抑制率达到79.3%～84.7%;抗氧化能力是trolox的1.1～1.5倍。     

脑海马结构研究进展

综述了海马结构研究的新进展,包括（１）磁共振成像海马结构体积测定;（２）微波辐射对海马的影响;（３）探讨主要由松果体分泌的褪黑素（ｍｅｌａｔｏｎｉｎ）与海马之间的功能关系;（４）在鼠海马内移植神经组织改善记忆减退;（５）铅、锌、铝离子对海马记忆的影响。