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41.
Hibi K Ushio H Fukuda H Mitsubayashi K Hayashi T Ren H Endo H 《Analytical and bioanalytical chemistry》2008,391(4):1147-1152
Bacterial cold water disease, caused by Flavobacterium psychrophilum, is a serious problem in the aquaculture industry worldwide. Several methods to prevent and treat cold water disease have
been studied. Although detection at the early stage of F. psychrophilum infection is very important for the prevention and treatment of cold water disease, an effective detection method has not
yet been developed. The use of flow cytometry (FCM) for the rapid determination of bacterial cell numbers with high sensitivity
is beginning to attract attention. Immunomagnetic separation (IMS) has also been used to detect F. psychrophilum. The purpose of the present study was to develop a method to quickly determine the number of bacterial cells by combining
the FCM and IMS methods. Because samples can be more effectively concentrated using smaller magnetic beads and stronger magnetism,
we used carbonyl iron powder as the magnetic beads for the IMS. The detection level of F. psychrophilum using FCM combined with IMS was 5 orders lower than that using FCM without IMS. The values determined using FCM combined
with IMS strongly correlated with those obtained using the colony-counting method, in the range of approximately 10–108 colony-forming units per milliliter. One FCM assay could be completed within 60 s and the total assay time, including sample
preparation, was less than 2 h. The combined method of FCM with IMS developed in this study can be used reliably for the rapid
detection of F. psychrophilum. 相似文献
42.
Dr. Kais Dhbaibi Maxime Grasser Dr. Haiet Douib Dr. Vincent Dorcet Prof. Olivier Cador Dr. Nicolas Vanthuyne Dr. François Riobé Dr. Olivier Maury Prof. Stéphan Guy Dr. Amina Bensalah-Ledoux Dr. Bruno Baguenard Prof. Geert L. J. A. Rikken Prof. Cyrille Train Dr. Boris Le Guennic Dr. Matteo Atzori Dr. Fabrice Pointillart Dr. Jeanne Crassous 《Angewandte Chemie (International ed. in English)》2023,62(5):e202215558
The combination of physical properties sensitive to molecular chirality in a single system allows the observation of fascinating phenomena such as magneto-chiral dichroism (MChD) and circularly polarized luminescence (CPL) having potential applications for optical data readout and display technology. Homochiral monodimensional coordination polymers of YbIII were designed from a 2,15-bis-ethynyl-hexahelicenic scaffold decorated with two terminal 4-pyridyl units. Thanks to the coordination of the chiral organic chromophore to Yb(hfac)3 units (hfac−=1,1,1,5,5,5-hexafluoroacetylaconate), efficient NIR-CPL activity is observed. Moreover, the specific crystal field around the YbIII induces a strong magnetic anisotropy which leads to a single-molecule magnet (SMM) behaviour and a remarkable room temperature MChD. The MChD-structural correlation is supported by computational investigations. 相似文献
43.
Optimal brushless DC motor design using genetic algorithms 总被引:2,自引:0,他引:2
This paper presents a method for the optimal design of a slotless permanent magnet brushless DC (BLDC) motor with surface mounted magnets using a genetic algorithm. Characteristics of the motor are expressed as functions of motor geometries. The objective function is a combination of losses, volume and cost to be minimized simultaneously. Electrical and mechanical requirements (i.e. voltage, torque and speed) and other limitations (e.g. upper and lower limits of the motor geometries) are cast into constraints of the optimization problem. One sample case is used to illustrate the design and optimization technique. 相似文献
44.
Dirk Plendl Marian Fujara Alexei F. Privalov Franz Fujara 《Journal of magnetic resonance (San Diego, Calif. : 1997)》2009,198(2):183-187
A new concept for an energy efficient electromagnet for fast field cycling NMR applications as well as its construction and first test results are presented. The magnet, which provides a rectangular sample space of 17 × 25 mm, has an iron yoke and pole pieces optimised with respect to the B0 homogeneity. The maximum field is 0.66 T at a current of 320 A; its field inhomogeneity for a cylindrical sample (length 7 mm, diameter 6 mm) is about 50 ppm. The power dissipation during polarisation at 0.55 T is as low as 1.4 kW. The magnet is powered by a commercially available power supply and can be rapidly switched with a slew rate of 0.55 T/ms. The system has shown a stability of 50 ppm/h. 相似文献
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46.
介绍了国家同步辐射实验室二期工程新建波动器UD-1的物理设计,给出了设计参数和主要技术要求。从储存环电子束流的要求和用户对光源的要求两方面分析了波动器磁场品质应满足的技术指标,给出UD 1的磁场一次积分值应小于2×10-4T·m,二次积分值应小于2×10-4T·m
2,相位误差应小于10°。 相似文献
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48.
科学技术的发展对于磁场强度质量的要求越来越高,极端强磁场条件是人类追求的永远的科学目标,它孕育着许多重大的科学发现和新技术的产生,对人类的科学和技术以及生活产生重大的影响.以磁体为核心与电力电子器件以及相关的软件等结合可以构成各种各样科学仪器和装置,广泛应用在科学研究和工业特种装备中.磁技术对于人类的科学与技术进步起到越来越重要的作用,尤其是极高磁场所带来的诸多优点,使得人类对于物质世界认识不断加深,对于生命的起源以及从事疾病的防治的研究有特别重要的意义.本文介绍磁体基本原理、磁场产生的方法与应用以及相关的发展. 相似文献
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50.
S.A. Romero M.F. de Campos H.R. Rechenberg F.P. Missell 《Journal of magnetism and magnetic materials》2008,320(14):e73-e76
Several magnets with different Zr contents were studied: Sm(CobalFe0.2Cu0.1Zrx)8 (bal=balance; x=0, 0.02, 0.04, 0.06 and 0.08). The microstructure of the magnets includes three main phases, all crystallographically coherent: the cell phase Sm2(Co,Fe)17, the cell boundary phase Sm(Co,Cu)5 and a lamellar Zr-rich phase, rhombohedral (ZrSm)1Co3. The hysteresis curves were compared with the Callen, Liu and Cullen (CLC) modification of the Stoner–Wohlfarth model for an isotropic distribution of interacting single-domain particles. Choosing reasonable values for the saturation magnetization Ms, the anisotropy field Ha, and the mean-field interactions of the CLC model, we were able to reproduce the main features of the hysteresis curves for the x=0.02 and 0.04 samples. For higher x values, X-ray diffraction Rietveld analysis revealed the presence of other “impurity” phases, among them cubic Zr6(Co,Fe)23, rhombohedral (SmZr)5(CoFeCu)19 and rhombohedral (SmZr)2(CoFeCu)7. 相似文献