Fatty acid composition of human erythrocyte membranes by capillary gas chromatography-mass spectrometry

Capillary gas chromatographic and gas chromatographic--mass spectrometric methods were employed for profiling total fatty acid content of human erythrocyte membranes. The protocol was designed to efficiently separate, identify, and accurately quantify the fatty acid composition in human erythrocyte membranes. Washed erythrocyte "ghosts" were saponified in aqueous methanolic sodium hydroxide solution and methylated with boron trichloride and acid catalysis. Extracted total fatty acid methyl esters (FAMEs) were analyzed using a highly polar cyanopropylsiloxane SP 2560 fused-silica capillary column. Total run time was 55 min, and 45 FAMEs were tentatively identified by relative retention times compared to those of known FAMEs. Confirmation of identities by mass spectral structure elucidation revealed saturated, mono- and polyunsaturated, and branched-chain FAMEs. The presence of four fatty aldehydes was also confirmed as dimethyl acetal derivatives. Identification of cis/trans isomers was based on relative retention times and characteristic profile of the cis/trans FAME standard. Quantification of FAMEs for normal subjects showed some variation in relative amounts, consistent with expectations based on literature reports on total or phospholipid FAMEs from human erythrocytes. Separation of individual components of fatty acid families (n-3), (n-6), and (n-9) is demonstrated. Losses in relative amounts of polyunsaturated fatty acids upon storing samples were also detectable by this rapid method.     

Monoreflections of Archimedean ?-groups, regular σ-frames and regular Lindelöf frames

We prove that in the category of Archimedean lattice-ordered groups with weak unit there is no homomorphism-closed monoreflection strictly between the strongest essential monoreflection (the so-called “closure under countable composition”) and the strongest monoreflection (the epicompletion). It follows that in the category of regular σ-frames, the only non-trivial monoreflective subcategory that is hereditary with respect to closed quotients consists of the boolean σ-algebras. Also, in the category of regular Lindelöf locales, there is only one non-trivial closed-hereditary epi-coreflection. The proof hinges on an elementary lemma about the kinds of discontinuities that are exhibited by the elements of a composition-closed l-group of real-valued functions on R.     

A protective layer on As2S3 film for photo-resist patterning

We have developed an effective fabrication process for As₂S₃ planar waveguides. A bottom anti-reflection coating provides a layer that not only protects the As₂S₃ film from attack by the alkaline developer but also improves line edge roughness of photo-resist patterns which is a pre-requisite for waveguides with smooth sidewalls.     

Chalcogenide glass photonic crystals

All-optical switching devices are based on a material possessing a nonlinear optical response, enabling light to control light, and are enjoying renewed interest. Photonic crystals are a promising platform for realizing compact all-optical switches operating at very low power and integrated on an optical integrated circuit. In this review, we show that by making photonic crystals from a highly nonlinear chalcogenide glass, we have the potential to integrate a variety of active devices into a photonic chip. We describe the fabrication and testing of two-dimensional Ge₃₃As₁₂ Se₅₅ chalcogenide glass photonic crystal membrane devices (waveguides and microcavities). We then demonstrate the ability to post-tune the devices using the material photosensitivity. In one proposal we hope to introduce a double-heterostructure microcavity using the photosensitivity alone.     

Micromotor endoscope catheter for in vivo, ultrahigh-resolution optical coherence tomography

A distally actuated, rotational-scanning micromotor endoscope catheter probe is demonstrated for ultrahigh-resolution in vivo endoscopic optical coherence tomography (OCT) imaging. The probe permits focus adjustment for visualization of tissue morphology at varying depths with improved transverse resolution compared with standard OCT imaging probes. The distal actuation avoids nonuniform scanning motion artifacts that are present with other probe designs and can permit a wider range of imaging speeds. Ultrahigh-resolution endoscopic imaging is demonstrated in a rabbit with <4-microm axial resolution by use of a femtosecond Cr:forsterite laser light source. The micromotor endoscope catheter probe promises to improve OCT imaging performance in future endoscopic imaging applications.     

Hydrogen chemisorption on silicon (100) 2 × 1

Changes in the valence band density of states (DOS) of a (100) silicon surface that accompany he chemisorption of atomic hydrogen onto that surface are deduced from a study of the changes in the L_2,3VV Auger lineshape. Complementary changes in the conduction band DOS are inferred from changes in L_2,3VV-core-level characteristic loss spectra (CLS). The chemisorbed hydrogen layer is identified as the dihydride phase from low energy electron diffraction measurements. Upon hydrogen adsorption the DOS at the top of the valence band decreases and new energy levels associated with the Si-H bonds appear lower in the band. Assuming that the Auger signal from the hydrogen covered sample consists of a superposition of a signal from silicon atoms bonded to hydrogen in the dihydride layer and an elemental-Si signal from the substrate, a N(E) difference spectrum with features due only to the dihydride is obtained by subtracting the background corrected, loss deconvoluted L_2,3VV signal for a clean (100)Si surface rom the corresponding signal for the hydrogen covered surface. Comparisons of the energy position of the major peak in this difference spectrum with that of the main peak in a gas phase silane Si-L_2,3VV spectrum, and of the corresponding Auger energy calculated empirically, indicate a hole—hole interaction energy of ~8 eV for the two-hole final state in the gaseous system and zero for the dihydride surface system. Hydrogen induced changes in the conduction band DOS are less apparent than those of the valence band DOS with only the possibility of a decrease in the DOS at the bottom of the conduction band being inferred from the CLS measurements. Electron stimulated desorption of hydrogen from the dihydride layer is adduced from changes in the Auger lineshape under electron beam irradiation of the surface. Hydrogen induced changes in the near-elastic electron energy loss spectra (ELS) are also reported and compared with previously published ELS results.     

Deuterium hyperfine structure in interstellar C3HD

The deuterium nuclear quadrupole hyperfine structure of the transition 1(10)-1(01) of the ring molecule cyclopropenylidene-d1 (C3HD) has been observed in emission from interstellar molecular clouds. The narrowest linewidths (approximately 7 kHz) so far observed are in the cloud L1498. The derived D coupling constants Xzz = 186.9(1.4) kHz, eta=0.063(18) agree well with correlations based on other molecules.     

On the second dielectric virial coefficients of methane and the inert gases

The induced quadrupole contribution to the distortion polarization term in the dielectric virial coefficient (B) has been evaluated for methane and the inert gases neon, argon, krypton and xenon. It is shown to be a substantial fraction of the experimental value of B for these systems. For argon the full ‘ classical ’ value of B is estimated by making use of a previous calculation of the classical overlap correction to the point polarizable particle model. It is shown that the classical value of B is in substantial agreement with experiment for this case.