Preparation and selective recognition of a novel solid‐phase microextraction fiber combined with molecularly imprinted polymers for the extraction of parabens in soy sample

A prepared molecularly imprinted polymer with ethyl p‐hydroxybenzoate as template molecule was applied for the first time to a homemade solid‐phase microextraction fiber. The molecularly imprinted polymer‐coated solid‐phase microextraction fiber was characterized by scanning electron microscopy and thermogravimetric analysis. Various parameters were investigated, including extraction temperature, extraction time, and desorption time. Under the optimum extraction conditions, the molecularly imprinted polymer‐coated solid‐phase microextraction fiber exhibited higher selectivity with greater extraction capacity toward parabens compared with the nonimprinted polymer‐coated solid‐phase microextraction fiber and commercial fibers. The molecularly imprinted polymer‐coated solid‐phase microextraction fiber was tested using gas chromatography to determine parabens, including methyl p‐hydroxybenzoate, ethyl p‐hydroxybenzoate, and propyl p‐hydroxybenzoate. The linear ranges were 0.01–10 μg/mL with a correlation coefficient above 0.9943. The detection limits (under signal‐to‐noise ratio of 3) were below 0.30 μg/L. The fiber was successfully applied to the simultaneous analysis of three parabens in spiked soy samples with satisfactory recoveries of 95.48, 97.86, and 92.17%, respectively. The relative standard deviations (n=6) were within 2.83–3.91%. The proposed molecularly imprinted polymer‐coated solid‐phase microextraction method is suitable for selective extraction and determination of trace parabens in food samples.     

Antioxidant peptides from <Emphasis Type="Italic">Cicer arietinum</Emphasis> of Xinjiang,China

Four peptides of molecular weights 1.148, 4.68, 5.41, and 9.086 kDa with antioxidant activity were isolated from chickpea sprout using two-step ion-exchange chromatographic and HPLC techniques for the first time. The partial N-terminal amino acid sequences of the peptide named YZDBCM-1 (9.086 kDa) was determined as H₂N^1-15-H₂N-Ala-Ile-Thr-Cys-Gly-Arg-Val-Ser-Ala-Ala-Leu-Ala-Pro-Pro-Leu using the Edman automated sequencing apparatus, which was a new peptide rich in alanine. It was shown that the antioxidant activity of the peptide YZDBCM-1 was IC₅₀ 156.2 μg/mL (17.2 μmol/L) to the free radical of ABTS.     

致密砂岩气藏纳微观结构及渗流特征

随着常规油气资源的减少,致密砂岩气藏逐渐成为勘探开发的热点.然而致密砂岩气高效产出机理研究还相对滞后,已经成为目前制约致密砂岩气大规模经济有效开发的瓶颈.致密砂岩由于其特殊的地质特征, 基质微观结构复杂,天然裂缝一定程度发育, 投产一般需要水力压裂等增产措施,气体在其中渗流存在跨尺度效应.研究揭示出致密砂岩气在多孔介质中的流动分为解吸、扩散、渗流等几个方式,包括浓度场下的扩散、压力场中的渗流等.综述了致密砂岩存在的非常规地质特征及其研究描述方法,并依据克努森数大小, 对致密砂岩气的渗流状态进行流态区域划分,为更好的理解和模拟致密砂岩气的流动状态提供了理论依据,并指出建立一种高效、简洁的微观结构精细描述方法,以及解决致密砂岩气在多尺度条件下的传质优化等是今后研究的重点方向.      

糖苷端差异构体的波谱研究(Ⅳ)——全乙酰化芳基1-硫代糖苷的~(13)C-NMR谱

本文报导了一系列全乙酰化邻甲基苯基1-硫代吡喃型五碳糖苷、六碳糖苷及双糖糖苷的~(13)C-NMR谱的化学位移数据,并进行了归属。比较了端差异构体间C_1位化学位移的差异;发现均为β型的高于α型的,为用~(13)C-NMR谱去区别全乙酰化芳基1-硫代糖苷提供一个依据。     

思维导图在分析化学中的教学应用探索

Taking the chapter of "errors and data processing" in analytical chemistry as an example, the teaching design, teaching practice and teaching efficiency of mind-mapping were explored. The derivation of the new knowledge point, the relation between the new and old knowledge points, their generalization and summarization were emphasized in the design of mind-mapping; the blackboard-writing and mixed-media complemented each other in the teaching practice, the guiding function of mind-mapping was highlighted; and students' logical thinking was practiced in homework. The teaching practice and questionnaire survey show that it is necessary to introduce mind-mapping to university classroom teaching. Mind-mapping can improve students' logical thinking, increase their "learning ability" and arouse their enthusiasm. The teaching effect is satisfying.     

Dually emitting gold-silver nanoclusters as viable ratiometric fluorescent probes for cysteine and arginine

Glutathione coated gold and silver nanoclusters (GSH-Au/AgNCs) were synthesized by one-pot reduction methods and are found to be viable fluorescent nanoprobes for cysteine (Cys) and arginine (Arg), with good selectivity over other amino acids. The GSH-Au/AgNCs have two emissions at 616 nm and 412 nm when excited at 360 nm. With the increased concentration of Cys, the ratio of the emission intensities (I₆₁₆/I₄₁₂) linearly decreases with Cys in concentration ranging from 0.05 to 10 μM and from 10 to 50 μM, respectively. With increased concentrations of Arg, the ratio of I₆₁₆/I₄₁₂ linearly decreases with Arg concentration ranging from 0 to 50 μM and from 50 to 100 μM, respectively. The probe was applied to the determination of Cys and Arg in spiked samples of serum and urine where it gave good recoveries.

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Graphical abstract Glutathione-coated gold and silver nanoclusters (GSH-Au/AgNCs) were synthesized by one-pot reduction and are found to be viable fluorescent nanoprobes for cysteine (Cys) and arginine (Arg).

     

Isolation of biocidal peptides from Anethum graveolens seeds

Three peptides with fungicidal activity with molecular weights 4–8 kDa were isolated from Anethum graveolens L. seeds. The cationic peptides were tested in vitro and inhibited the pathogenic strain Verticillium dahliae. The effective concentrations required for 50% inhibition (IC₅₀) growth of fungi were in the range 1.3–31 μg/mL. Peptide D-AFP-3 was the most active biocidal peptide. __________ Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 474–476, September–October, 2006.     

New peptide from seeds of <Emphasis Type="Italic">Cicer arietinum</Emphasis>

The pure peptide YZDCM was isolated from seeds of Cicer arietinum and assigned to the homologous class of defensins according to molecular weight and N-terminus amino-acid sequence.