Dimerization of haplamine

Institute of the Chemistry of Plant Substances, Uzbek SSR Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 51–53, January–February, 1989.     

Structure of the alkaloid kalidine

The spatial structure of the alkaloid kalidine obtained from the epigeal part ofKalidium gracile Fenzl. has been determined by IR and NMR spectroscopy and x-ray structural analysis. It has been established that it is the chloride of the trimethyl derivative of glycinamide.     

The structure of folifinine

Conclusions 1. A new base, folifinine, has been isolated from the epigeal part ofHaplophyllum foliosum Vved. Its developed formula is C₁₆H₁₄N(OH)₂ (OCH₃) (-O-).2. The structure 8-hydroxy-7-(3-hydroxy-3-methylbutyl)-4-methoxy-2, 3-furoquinoline has been proposed for folifinine.Khimiya Prirodnykh Soedinenii, Vol. 4, No. 6. pp. 373–376, 1968     

Electrophoretic determination of albumin in urine using on-line concentration techniques

To improve the sensitivity of the UV-detection for the determination of trace amounts of albumin by capillary zone electrophoresis (CZE), five on-line preconcentration techniques, including field-amplified sample stacking (FASS), head-column field-amplified sample stacking (HC-FASS), stacking with a polymer solution, dynamic pH junction and large volume sample stacking (LVSS) with reversed polarity, were compared. Sensitivity enhancement factor and reproducibility were two factors that were used to assess the suitability of each method. To minimize protein adsorption on the capillary wall, capillaries were covalently modified with anionic polymer, poly(sulfopropylmethacrylate) coating. All used methods have good reproducibility. The maximum sensitivity enhancement factor (about 67-fold in terms of peak heights) was achieved with LVSS technique. The concentration limit of detection (LOD) (S/N=3) for the human serum albumin obtained with the optimized LVSS approach was 15 microg/ml with UV-detection. The method was further evaluated for the analysis of urine samples with gel-filtration-based sample-desalting procedure.     

Synthesis and study of the properties of PLOT columns based on new dendritic polymers for the separation of proteins by capillary electrochromatography

Capillary electrochromatography (CEC) is actively used for biomedical studies. Therefore, the search for new materials for stationary and pseudostationary phases for CEC that allow the control of the selectivity of separation, modification of quarts capillary walls, an increase in efficiency, and the reduction of the limits of analyte detection is a promising line of research. PLOT columns in this work have been synthesized using new dendritic polymers that are water soluble oligosaccharide derivatives of hyperbranched polyethyleneimine (PEI-Mal). The obtained coating has been characterized by the value of electroosmotic flow (EOF), and the influence of the composition and pH of a buffer electrolyte on the separation of proteins under CEC conditions with PLOT columns has been studied.