Visualization and In Situ Ablation of Intracellular Bacterial Pathogens through Metabolic Labeling

Protected by the host cells, the hidden intracellular bacteria are typically difficult to kill by common antibiotics and cannot be visualized without complex cellular pretreatments. Herein, we successfully developed a bacteria-metabolizable dual-functional probe TPEPy-d -Ala, which is based on d -alanine and a photosensitizer with aggregation-induced emission for fluorescence turn-on imaging of intracellular bacteria in living host cells and photodynamic ablation in situ. Once metabolically incorporated into bacterial peptidoglycan, the intramolecular motions of TPEPy-d -Ala are inhibited, leading to an enhanced fluorescent signal, which allows the clear visualization of the intracellular bacteria. Moreover, TPEPy-d -Ala can effectively ablate the labeled intracellular bacteria in situ owing to covalent ligation to peptidoglycan, yielding a low intracellular minimum inhibitory concentration (MIC) of 20±0.5 μg mL⁻¹, much more efficient than that of a commonly used antibiotic, vancomycin.     

Visualization and In Situ Ablation of Intracellular Bacterial Pathogens through Metabolic Labeling

Protected by the host cells, the hidden intracellular bacteria are typically difficult to kill by common antibiotics and cannot be visualized without complex cellular pretreatments. Herein, we successfully developed a bacteria‐metabolizable dual‐functional probe TPEPy‐d ‐Ala, which is based on d ‐alanine and a photosensitizer with aggregation‐induced emission for fluorescence turn‐on imaging of intracellular bacteria in living host cells and photodynamic ablation in situ. Once metabolically incorporated into bacterial peptidoglycan, the intramolecular motions of TPEPy‐d ‐Ala are inhibited, leading to an enhanced fluorescent signal, which allows the clear visualization of the intracellular bacteria. Moreover, TPEPy‐d ‐Ala can effectively ablate the labeled intracellular bacteria in situ owing to covalent ligation to peptidoglycan, yielding a low intracellular minimum inhibitory concentration (MIC) of 20±0.5 μg mL^?1, much more efficient than that of a commonly used antibiotic, vancomycin.     

Full use of the liquid nature of the stationary phase: The development of elution‐extrusion counter current chromatography

Elution‐extrusion counter current chromatography extrudes the most solute retained in the column with the highest possible peak resolution. It can greatly improve the hydrophobic window. In recent years, elution‐extrusion counter current chromatography has received extensive attention in the separation of complex samples. This article first reviews the development and application of elution‐extrusion counter current chromatography, including its origin, mechanism, advantages and disadvantages, and some representative applications. At the same time, this review also shared our visions and ideas on how to improve the elution‐extrusion mode. This article aims to provide certain reference for the research of this technology.     

Research Progress in the Field of Adsorption and Catalytic Degradation of Sewage by Hydrotalcite‐Derived Materials

With the rapid development of industry and agriculture and the greatly improved living conditions, the resultant gradually deteriorated environments threaten the human beings. Refractory or even toxic pollutants, which are from different industries such as printing and dyeing, pesticides, chemicals, petrochemicals, plastics and rubber, seriously threat the ecosystems and human health. Having the advantages of flexible composition, unique structure, high stability, memory effect, easy preparation and low cost, hydrotalcite compounds have a great potential in sewage degradation and environmental protection. This study focuses on the adsorption and catalytic properties (such as photocatalysis, electrocatalysis and photoelectrocatalysis) of hydrotalcite‐derived materials for treating organic, inorganic and heavy metal ion sewage. The types of adsorption and catalysis, and the effects of various influencing factors on the degradation efficiency were discussed as well.     

圆锥透镜对偏轴球面波光束的衍射模式

球面波入射圆锥透镜产生的出射光束中心光斑直径较小且焦深长，适合通讯领域长距离测量和准直。针对实际操作中球面波光束入射时很可能与圆锥透镜的中心轴线发生偏移或倾斜，使衍射光场发生变化，着重研究圆锥透镜对偏轴球面波光束的衍射模式，并通过衍射理论和稳相法分析，研究其径向衍射光场的分布特性。使用半径为15mm，底角为1°的圆锥透镜进行计算机仿真和实验，最后将实验结果与理论分析和仿真结果相比较，证明球面波的曲率半径和出射光束的传输距离越小，偏轴对衍射模式的影响越小。该研究结果表明：在激光通讯等长距离通信中，使用曲率半径小的球面波光束代替平面波入射圆锥透镜，不仅可以扩展焦深，还可以降低偏轴对测量的影响。     

Lingshuine,an Unexpected Passerini Product from the Hainan Sponge Axinyssa variabilis

Lingshuine ( 1 ), a novel sesquiterpene amide, along with three known N‐containing compounds, 2 – 4 , have been isolated from the Hainan sponge Axinyssa variabilis. The structure of 1 was elucidated by detailed analysis of the spectroscopic data and by chemical methods. Lingshuine represents the first example of a Passerini product formed during the isolation process.     

A novel pentaazadentate bismacrocycle cadmium complex

A novel pentaazadentate bismacrocycle was synthesized through an improved procedure of 1:1 Schiff base condensation of diformyltripyrrane with 3,4,3′,4′‐tetraamino‐biphenyl hydrochloride using Pb²⁺ as template ion, in which the condensed byproduct water was removed efficiently to make the yield over 90%. Then the bismacrocycle legend reacted with cadmium chloride to yield the title metal complex with molecular weight being 1200.4 by TOF MS and the λ_max being 766 nm.     

Pure single line oscillation of HF lasers in fine atmospheric window

Applied Physics B - A correction to this paper has been published: https://doi.org/10.1007/s00340-021-07611-y     

影响石英单模光纤数值孔径测量的因素及分析

本文采用远场光斑法和光强法测量了石英单模光纤的数值孔径.实验结果表明,由光纤耦合条件不同导致的光斑形状变化,对数值孔径的测量影响不大,单模光纤的纤芯直径小,导致出射光存在较强的衍射现象,对数值孔径的测量造成较大的影响,无论采用光斑法还是光强法,需以衍射第1次极大为计算标准,尽量选择光斑中心为亮斑时测量其数值孔径.此外,用远场光斑法进行测量时,观察屏到光纤的距离要合适,太短或太长都会增加实验误差.