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181.
We have investigated the effect of ionic strength on the aggregation behavior of n-dodecyl phosphocholine. On the basis of the classical Corrin–Harkins relation, the critical micellar concentration of this
detergent decreases with a biphasic trend on lithium chloride addition. It is nearly constant below 150 mM salt, with a mean
value of 0.91 mM, whereas it undergoes a dramatic 80-fold decrease in 7 M LiCl. Such a drop in the critical micellar concentration
could be explained by the effect of salting out and the implication of phosphocholine head groups on the organization of surrounding
water. Knowledge of the effective critical micellar concentration of n-dodecyl phosphocholine could be useful in the purification of membrane proteins in non-denaturing conditions. 相似文献
182.
We give a unified approach to study the existence of multiple positive solutions of nonlinear differential inclusions of the form subject to various nonlocal boundary conditions. We study these problems via a perturbed integral inclusion of the form . 相似文献
183.
Carmen Tesoro Filomena Lelario Rosanna Ciriello Giuliana Bianco Maria Assunta Acquavia Paola Montoro Maria Assunta Crescenzi Emanuela Gregori Mario Dell'Agli Stefano Piazza Antonio Guerrieri Angela Di Capua 《Journal of mass spectrometry : JMS》2023,58(10):e4952
An analytical method based on ultrasound assisted extraction (UAE) and liquid chromatography coupled to electrospray tandem mass spectrometry (LC–ESI/MS/MS) was validated and applied for determining L-dopa in four ecotypes of Fagioli di Sarconi beans (Phaseolus vulgaris L.), marked with the European label PGI (Protected Geographical Indication). The selectivity of the proposed method was ensured by the specific fragmentation of the analyte. Simple isocratic chromatographic conditions and mass spectrometric detection in multiple reaction monitoring (MRM) acquisition mode were used for sensitive quantification. The LC–ESI/MS/MS method was validated within a linear range of 0.001–5.000 μg/mL. Values of 0.4 and 1.1 ng/mL were obtained for the limits of detection and quantification, respectively. The repeatability, inter-day precision, and recovery values ranges were 0.6%–4.5%, 5.4%–9.9%, and 83%–93%, respectively. Fresh and dried beans, as well as pods, cultivated exclusively with organic methods avoiding any synthetic fertilizers and pesticides were analyzed showing an L-dopa content ranging from 0.020 ± 0.005 to 2.34 ± 0.05 μg/g dry weight. 相似文献