硫堇/Nafion/石墨烯修饰电极对NADH的电催化活性研究

为了提高电化学检测NADH的分析性能,制备了硫堇/Nafion/石墨烯修饰的玻碳电极(GCE),利用Nifion的强离子交换能力将硫堇修饰在电极表面,形成硫堇/Nafion/石墨烯复合电极.与裸玻碳电极相比,在NADH的反应中观察到了电位明显降低和电流响应显著增大.证实了硫堇/Nafion/石墨烯复合电极对NADH有协同的电催化能力.为构造有效地检测NADH的电化学传感器提供一个良好的应用平台.     

PAA-supported Hantzsch 1,4-dihydropyridine ester: an efficient catalyst for the hydrogenation of α,β-epoxy ketones

A new type of water-soluble polymer-supported NADH co-enzyme model-PAA (polyacrylic acid)-supported Hantzsch 1,4-dihydropyridine ester (PAA–HEH) was designed and synthesized. Catalytic amount of the supported reagent was used in the hydrogenation of α,β-epoxy ketones to the corresponding β-hydroxy ketones and showed great catalytic efficiency in the reduction reaction. This PAA–HEH was an optimal potential for recycling use.     

Effects of Carbon Nanotube Paper Properties on Enzymatic Bioanodes

Carbon papers are frequent current collectors in fuel cells, but recently carbon nanotube papers have been introduced as new types of carbon paper. They have varying thickness, surface area, density, gas permeability, and conductivity. This paper studies the effect of these properties on electrochemical performance. Electropolymerizing methylene green on each carbon nanotube papers was performed to study the papers’ ability to oxidize NADH. Secondly, glucose bioanodes were formed with carbon nanotube papers to study glucose bioelectrocatalysis. The optimal carbon nanotube paper had a high surface area, low gas permeability, and low sheet resistance. Our results showed a greater than three‐fold increase in sensitivity over commercially available Toray carbon paper.     

Electrochemical Polymerization of Azure Blue II and Its Electrocatalytic Activity toward NADH Oxidation

IntroductionOver 30 0dehydrogenasesareknowntobedependentonthenicotiamideadeninedinucleotidecoenzymeinitsreduced (NADH )andoxidized (NAD+)forms ,andamonitoringofenzymaticallygeneratedNADHisthebasisforthedevelopmentofbiosensorsfortheselecteddehydro genase .Hence ,thedetectionofthiscoenzymeisimpor tantinbothpracticalanalysisandbiochemicalsynthesis .ThereportedoverpotentialforoxidationofNADHatpH7 0isabout 1 1Vatcarbonand 1 3Vatplatinumelec trodes .1,2 Itisgenerallyacceptedthatthedirecto…     

Pyridylidene‐Mediated Dihydrogen Activation Coupled with Catalytic Imine Reduction

In recent years, dihydrogen activation at non‐metallic centers has received increasing attention. A system in which dihydrogen is trapped by a pyridylidene intermediate that is generated from a pyridinium salt and a base is now reported. The dihydropyridine formed in this process can act as reducing agent towards organic electrophiles. By coupling the hydrogen‐activation step with subsequent hydride transfer from the dihydropyridine to an imine, a catalytic process was established. Treatment of the N‐phenylimine of phenyl trifluoromethyl ketone with 5–20 mol % of N‐mesityl‐3,5‐bis(2,6‐dimethylphenyl)pyridinium triflate and 0.3–1.0 equivalents of LiN(SiMe₃)₂ under 50 bar of hydrogen gas resulted in high conversion into the corresponding amine.     

催化荧光法研究辅酶NADH的抗氧化性能

采用催化荧光法观察了3种不同类型的活性氧物质(H2O2,.OH,ONOO-)与辅酶NADH的相互作用.研究表明,NADH具有明显的清除活性氧的功能,且对不同类型活性氧的清除作用表现不同:对.OH的清除作用最为迅速,对H2O2,ONOO-的清除作用则较为缓慢.进一步的研究发现,生物体内存在的肌红蛋白因具有过氧化物酶的特性可使清除H2O2的反应大大加速.     

Electrocatalytic Oxidation of NADH Based on Self-assembled Colloidal Gold and Nafion Matrixes and Co Complex Mediator

Nicotinamide adenine dinucleotide (NAD ) and its reduced form NADH used as coenzymes by over 300 dehydrogenases have been the subjects of numerous studies. Direct oxidation of NADH at a bare electrode only proceeds at high overpotentials, which finally le…