用氧电极结合枯草杆菌M45(Rec-)和H17(Rec+)快速鉴别金属离子的致癌性

Asimplified, efficient method has been constructed by combining O2 electrode andBacillus subtilis rec-assay strains to detect genetoxicity of various metal ions. The resultsshow that this method can be used to detect genotoxic metal compounds with a high sensitivity and fidelity, and the carcinogenicity of Cr6+, Co2+, Cd2+, Mn2+ ions are confirmed. Themanipulations are very simple, and reproducible responses was gained within half an hour.So this method can be applied to the screening of environmental metal compounds.     

膦、胂叶立德的化学与应用——Ⅳ.乙酰基亚甲基三苯基膦、胂与2-全氟炔酸甲酯的反应及其加合产物水解的研究

前文曾报道苯甲酰基亚甲基三苯基瞵(?)胂(1,M=P,As,R=C_6H_5)与2-全氟炔酸甲酯(2)的反应,主要产物为3(R=C_6H_5)和少量的4(R=C_6H_5);通过胂加合产物3(R=C_6H_5)     

再生丝素固定葡萄糖氧化酶及其传感器应用

再生丝素固定葡萄糖氧化酶及其传感器应用钱江红，刘永成，刘海鹰，于同隐，邓家祺（复旦大学化学系高分子科学系，上海，２００４３３）关键词再生丝素，葡萄糖氧化酶，传感器，酶电极酶电报的各项性能在很大程度上取决于酶的固定比方法，葡萄糖氧化酶的固定化方法很多１...     

气相色谱固定液极性和选择性评价

对非同族化合物溶质在两种固定相上保留值的关系进行了理论推导，并将该方程应用于８９种常见固定相的极性和选择性的评价。固定相的极性可用Ｂ值来评价，而选择性则用Ｂ、Ｔ３＾ａ，Ｃ３β值来评价。用该方程评价固定相具有两个优点，一是基于分子间作用力来评价固定相；二是探针化合物的选择不再受限制。     

用再生丝素固定过氧化物酶及其传感器在有机相中的研究

用再生丝素固定过氧化物酶及其传感器在有机相中的研究钱江红，刘永成，刘海鹰，于同隐，邓家祺（复旦大学化学系、高分子科学系，上海，２００４３３）关键词再生丝素，生物传感器，过氧化氢酶在非水介质中的催化活性已有研究［１］，生物传感器在有机相中有许多优点：如...     

Biofunctional Janus particles promote phagocytosis of tumor cells by macrophages

Herein, a versatile strategy for the construction of biofunctional Janus particles (JPs) through the combination of Pickering emulsion and copper-free click chemistry is developed for the study of particle-mediated cell–cell interactions. A variety of biomolecules including bovine serum albumin (BSA), ferritin, transferrin (Tf), and anti-signal regulatory protein alpha antibodies (aSIRPα), etc., can be incorporated into the Janus platform in a spatially defined manner. JPs consisting of Tf and aSIRPα (Tf–SPA1–aSIRPα JPs) demonstrate a significantly improved binding affinity to either macrophages or tumor cells compared to their uniformly modified counterparts. More importantly, Tf–SPA1–aSIRPα JPs mediate more efficient phagocytosis of tumor cells by macrophages as revealed by real-time high-content confocal microscopy. This study demonstrates the potential advantages of JPs in mediating cell–cell interactions and may contribute to the emerging cancer immunotherapy.

A versatile Janus particle platform modified with biological ligands can facilitate tumor cell phagocytosis by macrophages for promising cancer immunotherapy.     

基于自适应本征正交分解混合模型的跨音速流场分析

采用非线性模型替代线性回归模型响应本征正交分解(POD)基函数的系数,并采用自适应抽样方法确定快照集合,实现了基于自适应POD混合模型的跨音速叶片复杂流动分析及流场拟合.首先通过比较基于线性回归模型和非线性回归模型的基函数系数响应精度,验证非线性回归模型的收敛性和精确性;之后通过与静态抽样方法进行对比,研究分析自适应抽样技术的优越性;最后开展基于自适应POD混合模型的全三维跨音速流场分析及流动拟合.结果表明,采用自适应POD混合模型,不仅能够清晰地识别三维跨音速流场中的敏感流动特征,还能精确地拟合设计空间内任意状态的流场及出口气动参数.     

On the highest oxidation states of the actinoids in AnO4 molecules (An = Ac – Cm): A DMRG-CASSCF study

Actinoid tetroxide molecules AnO₄ (An = Ac – Cm) are investigated with the ab initio density matrix renormalization group (DMRG) approach. Natural orbital shapes are used to read out the oxidation state (OS) of the f-elements, and the atomic orbital energies and radii are used to explain the trends. The highest OSs reveal a “volcano”-type variation: For An = Ac – Np, the OSs are equal to the number of available valence electrons, that is, Ac^III, Th^IV, Pa^V, U^VI, and Np^VII. Starting with plutonium as the turning point, the highest OSs in the most stable AnO₄ isomers then decrease as Pu^V, Am^V, and Cm^III, indicating that the 5f-electrons are hard to be fully oxidized off from Pu onward. The variations are related to the actinoid contraction and to the 5f-covalency characteristics. Combined with previous work on OSs, we review their general trends throughout the periodic table, providing fundamental understanding of OS-relevant phenomena.     

高效液相色谱法检测肉类食品中4种嘌呤碱

建立了肉类食品中嘌呤含量的多组分高效液相色谱分析方法。采用V(三氟乙酸)∶V(甲酸)∶V(水)=5∶5∶1溶液在90℃下水解样品12min,水解液浓缩后过膜,以0.02mol/LKH2PO4(pH=3.8)为流动相进行色谱分离;流速1.0mL/min;柱温30℃;DAD二极管矩阵检测器检测,检测波长254nm;进样量10μL。在0.4～40mg/L的浓度范围内,各嘌呤的响应峰面积与其相应浓度呈良好相关性,r>0.9999,通过水解条件实验证实,90℃下12min能够使样品中嘌呤完全水解为游离态,且不破坏嘌呤物质,相对标准偏差<16.0%。本方法分离度好,能够快速检测肉类食品中4种主要的嘌呤组分。     

对硝基苯酚在酸性介质中的电化学还原反应机理

采用电化学原位红外光谱技术研究了对硝基苯酚在铜电极表面及硫酸介质中的电化学反应机理. 对硝基苯酚首先经电还原反应生成对亚硝基苯酚和对羟胺苯酚, 对亚硝基苯酚可发生缔合生成反式二聚体, 最后在较低的电位下, 对羟胺苯酚经电还原生成了对氨基苯酚.