Quenching effect of nickel ions on fluorescent gold nanoparticles

Herein, we reported the quenching effect of Ni(2+) on bovine serum albumin protected fluorescent gold nanoparticles (BSA-GNPs). The quenching mechanism was discussed and a static quenching mechanism was proposed. The number of binding sites (n), apparent stability constants (K) and corresponding thermodynamic parameters of BSA-GNPs-Ni(2+) complex were measured at different temperatures. Under optimum conditions, the fluorescence intensity of BSA-GNPs is linearly proportional to nickel concentration from 6.0x10(-8)mol/L to 8.0x10(-6)mol/L with a detection limit of 1.0x10(-8)mol/L. The result indicated that BSA-GNP was a potential Ni(2+) probe.     

流动注射光纤动力学荧光法测定锰──锰-二元荧光配合物-高碘酸钠体系

对二元荧光配合物作为动力学反应指示剂的可行性进行了考察,以8-羟基喹啉-5-磺酸铝-高碘酸钠为指示反应,建立起流动注射光导纤维光谱技术测定痕量锰(Ⅱ)的动力学荧光分析新体系.此法线性范围为5～200ng/mL,检出限为1ng/mL,对50ng/mL锰(Ⅱ)进行11次测定的相对标准偏差为0.5%,分析速度为45样次/h.