Isolation, Mapping, DNA Sequence and RFLPs Studies of Random Single-Copy DNA Segments on Human X Chromosome

Using the total human/mouse DNA as the probe, screening has been carried out three times with in situ plaque hybridization to obtain the single-copy DNA sequence from the human X chromosome genomic library. The effective rate of screening is 1. 45%. DNAs from clones containing single-copy inserts have been analyzed by a panel of hybrid cells with or without human X chromosome. Three segments, designated by DXFD52,73,75, are mapped to the X chromosome. DXFD52 has been precisely localized on Xq12-q13 with in situ chromosomal hybridization. DXFD52 has been partially sequenced. The results indicate that DXFD52 is a new isolated single-copy segment on the X chromosome. Great progress in the RFLPs study with DXFD52 has been achieved in the population of Chongqing, Sichuan Province. The results show that the DXFD52 can be used to detect the RFLP with Hind Ⅲ, Bgl Ⅱ, and Hinf Ⅰ. DXFD52 will be a potential "landmark" for the construction of the complete linkage map of human genome and the analysis of genomic s     

满江红鱼腥藻glnA基因上游调控区的克隆及其序列分析

以满江红鱼腥藻（Ａａｃ）提取总ＤＮＡ为模板,通过ＰＣＲ技术,扩增得到其谷氨酰胺合成酶基因（ｇｌｎＡ）上游区．经酶切得到４０９ｂｐ的片段,并将其连接到ｐＢｌｕｅｓｃｒｉｐｔⅡｋｓ＋上测序．将测序结果与Ａｎａｂａｅｎａ７１２０调控序列比较,有９８２％的同源性．在上游调控中也具有ｎｉｆｌｉｋｅ和Ｅ．ｃｏｌｉｌｉｋｅ启动子,值得注意的是,调节蛋白ＶＦ１的结合位点正好位于ｎｉｆｌｉｋｅ启动子上．所克隆的片段不但对蓝藻固氮、泌氨的基因调控研究具有意义,也对表达载体的构建具有实用价值．     

Cloning, construction of prokaryotic expression vector and expression ofEscherichia coli cytosine deaminase gene

Cytosine deaminase gene ofEscherichia coli strain H-30 was cloned, and its initiation codon of ‘GTG’ was mutated to ‘ATG’ by PCR. Prokaryotic recombinant expression vector pBV220-CD was constructed. Clone with high enzyme activity were selected by detecting their specific activity of cytosine deaminase. 5-FC(5-FC, 5-fluorocytosine) could induce the lethal toxicity to cells containing active CD gene. DNA sequence analysis indicated that there were 16 altered bases and 5 of them resulted in the alteration of amino acids in predicted peptide by comparing DNA sequence of the clone H-30-CD-11 with high enzyme activity with CD gene reported in Gene Bank.     

Research advances in gene therapy for Parkinson's disease

During the long period of time when people have been seeking for an effective therapeutic method for PD, the gene therapy has shown greater and greater advantages over other methods. It can be performed mainly in two ways: ex vivo and in vivo. With the former, TH gene as well as some neurotrophic factor genes (such as GDNF, BNDF genes) can be invited to some cell lines or primary cells thus forming engineered cells and then implanting them into brain. While with the latter, viral vectors including HSV-1, Ad, AAV that can be utilized to construct recombinant viruses, or non-virus vectors can be used to delived DNA into brain directly. The present review summarizes the recent research advances in the gene therapy for PD, and it is reasonable for us to predict a notable progress in prevention and treatment for PD in the next decade.     

Cloning and expression of the vp39 gene ofBombyx mori nuclear polyhedrosis virus inE. coli

The nuclear capsid protein gene (vp39) ofBombyx mori nuclear polyhedrosis virus (BmNPV) was amplified successfully by PCR technique and inserted into pGEM 3zf(+). The 5′ and 3′ terminal area of the amplified vp39 gene were sequenced with silver-staining dideoxy method. Bmvp39 gene was sub-cloned into the expression vector pRSET-A, and transformed intoE. coli BL21. This gene was highly expressed by IPTG induction. SDS-PAGE analysis showed that the expressed protein is about 38 kd, and the expressed amount reached maxium in 4 h with IPTG induction. Supported by the National Natural science Foundation of China and the Doctoral Foundation of Edn carto Committee Liu Deli: born in 1954, Doctoral Candidate from Huazhong Normal University To whom correspondence should be addressed: (027-7882712-2938)     

提高注射法导入外源基因棉铃成铃率的技术研究

对采用注射法导入外源基因的棉铃,在激素种类、不同生态条件、结铃部位、结铃性、整枝方式等方面进行分析,探讨了导入棉铃落铃机理和提高导入棉铃成铃率的技术途径。     

敏感和抗性的白血病HL60细胞对staurosporine诱导凋亡的不同反应(英)

用对蛋白激酶具有强烈抑制、作用广泛的抑制剂staurosporine（Sta）,研究敏感和抗三尖杉酯碱的人白血病HL60细胞中凋亡和多药抗药性的关系．Sta均能诱导2种细胞发生典型的凋亡,但抗性细胞发生凋亡需更长的时间,凋亡的细胞数减少．Sta增加柔红霉素在抗性细胞内的积聚,说明其能逆转多药抗药性．在抗性细胞凋亡过程中,mdrl基因表达没有变化,c-myc基因表达稍有增加．结果显示：Sta能诱导敏感和抗三尖杉酯碱的HL60细胞发生凋亡,mdrl基因表达与凋亡过程无关．     

上海地区汉族人单胺氧化酶基因微卫星DNA多态性研究

应用扩增片段长度多态性技术,分析单胺氧化酶基因的ＭＡＯＡ（ＣＡ）。力ＭＡＯＢＩ（ＧＴ）ｎ２个基因座的微卫星ＤＮＡ多态性。在２４６名（男性１２２人,女性１２４人）无血缘关系上海地区汉族人中检出ＭＡＯＡ（ＣＡ）ｎ基因座８－种等位基因（１１０－１２４ｂｐ）和１９种基因型。     

外源基因（bar）在转基因燕麦（AvenasativaL.）后代中的遗传与表达

通过微弹轰击获得的含有ｂａｒ基因的转基因燕麦植株自交产生转基因后代。利用ＰＣＲ方法检测ｂａｒ基因在后代中的传递情况。