Interfacially Bridging Covalent Network Yields Hyperstable and Ultralong Virus-Based Fibers for Engineering Functional Materials

We present a strategy of interfacially bridging covalent network within tobacco mosaic virus (TMV) virus-like particles (VLPs). We arranged T103C cysteine to laterally conjugate adjacent subunits. In the axis direction, we set A74C mutation and systematically investigated candidate from E50C to P54C as the other thiol function site, for forming longitudinal disulfide bond chains. Significantly, the T103C-TMV-E50C-A74C shows the highest robustness in assembly capability and structural stability with the largest length, for TMV VLP to date. The fibers with lengths from several to a dozen of micrometers even survive under pH 13. The robust nature of this TMV VLP allows for reducer-free synthesis of excellent electrocatalysts for application in harshly alkaline hydrogen evolution.     

Encapsulation of adefovir bis(l-leucine propyl)ester pro-virucide in cucurbit[7]uril and its activity against tobacco mosaic virus

The interaction of cucurbit[7]uril (Q[7]) with a pro-virucide, adefovir bis(l-leucine propyl)ester (PMEA-Leu) in aqueous solutions and in solid state was studied by ¹H NMR, UV absorption spectroscopy, fluorescence and IR spectroscopy. The ¹H NMR revealed that the leucine propyl moiety of the compound could be entrapped in the cavity of the host Q[7], and the other moiety except for leucine propyl moieties, including aminopurine, was probably located at the portal area of Q[7]. Absorption and fluorescence spectroscopy proved that the interaction of Q[7] with PMEA-Leu led to the formation of host–guest inclusion complexes (2:1) that were controlled by the concentration of the host Q[7]. Formation of the inclusion complex between Q[7] and PMEA-Leu was confirmed by IR spectroscopy in solid state. In addition, deliquescent stability studies indicated that the moisture stability of the host–guest complex was significantly enhanced. The phenomenon was explained by the fact that the formation of solid inclusion complexes can prevent the compounds from absorbing water. Finally, bioactivity of PMEA-Leu and its inclusion complex against tobacco mosaic virus (TMV) was tested. The compound PMEA-Leu and its inclusion complexes showed some inhibitory activity against TMV at 500 μg/ml in vivo.     

Efficient synthesis of benzimidazolyl-phenoxyacetic acid O-acetylxylopyranosyl and O-acetylgalactopyranosyl esters and their antiviral activity

Six benzimidazolylphenoxyacetic acid O-acetylxylopyranosyl and O-acetylgalactopyranosyl esters were synthesized through esterification reactions at room temperature using 4-dimethylaminopyridine as a catalyst and triethylamine as a deacidification reagent. Their structure was confirmed by IR, ¹H NMR spectra, MS, and elemental analysis. The synthesized compounds are all of β-configuration. The results show that DMAP is an effective catalyst; the yields can reach 63.6%. The above esters showed improved antiviral activity against tobacco mosaic virus. Published in Khimiya Geterotsiklicheskikh Soedinenii, No. 6, pp. 832–837, June, 2006.     

Replication of tobamovirus RNA

Tobamovirus is a positive-strand RNA virus of plants. Its single-stranded RNA genome replicates via the negative-strand RNA. In this review, we describe our current knowledge about viral and host factors associated with tobamovirus RNA replication and discuss the replication mechanisms. We also mention the usefulness of tobamovirus genomes as vectors.     

湿敏半导体陶瓷感湿机理的理论新模型

本详细论述了湿敏半导体陶瓷的微观感湿机理，讨论了比较接近于实际的“镶嵌模型”，给出了新模型的理论方程，为各种多孔陶瓷湿敏元件的开发提供了新的理论依据。     

一种基于特征不变描述的图像无缝拼接算法

针对尺度变化不明显场景的拼接,提出一种基于特征不变描述的图像无缝拼接算法.利用Harris特征检测算子进行特征点提取,在此基础上对提取出的特征点采用SIFT描述子进行处理,使其具备旋转不变性,然后利用k-d树算法进行遍历搜索定位,并用RANSAC算法进行图像间单应性变换矩阵计算,最后利用加权平均的融合方法进行图像无缝平滑,得到无缝拼接图像.实验结果表明:该算法在图像任意角度旋转的情况下,能有效地实现图像无缝拼接,并具有较强的鲁棒性.     

微流控振荡流反转录聚合酶链式反应快速检测烟草花叶病毒

建立了一种基于毛细管的振荡流反转录聚合酶链式反应(RT-PCR)的微流控装置检测烟草花叶病毒(TMV)的新方法.根据TMV移动蛋白基因设计一对PCR引物,对粗提纯TMV颗粒直接进行RT-PCR.用0.025%小牛血清蛋白(BSA)对反应毛细管内壁进行静力学和动力学钝化,提高了PCR效率.在此微流控装置上进行RT-PCR,流速为70 μL/min时, 检出限为0.01 μg cDNA;可以在17 min内成功扩增出179 bp的目的DNA片段.     

大麦抗黄花叶病的遗传方式和育种策略

本文通过5个抗病品种、2个感病品种的相互杂交,对F_1、F_2、BC_1、BC_2及多年的数据分析表明大麦抗BYMV抗性的遗传方式较复杂,不呈单基因控制的孟德尔遗传方式,而符合多基因控制的数量遗传模式。根据分析结果,初步讨论了大麦抗BYMV的育种策略。     

Testing the Possibility of Average-Color Perception from Multi-Colored Patterns

To test the possibility that an average color is perceived from a multi-colored pattern, the appearance of colored mosaic patterns as a whole was measured by the method of adjustment. As the range of color distribution in a mosaic pattern became larger, subjects matches deviated more from the photometric averages of each pattern and the subjects perceived higher brightness from mosaic patterns. Since the saliency of element colors was different in an equiluminant mosaic pattern, two possible hypotheses for selecting a color which represents a mosaic pattern was examined by using brightness match results for each element color. The results show that the mosaic element with the highest-saturation strongly influences the choice of a color to represent a mosaic pattern.