水溶性共轭聚合物研究与应用进展

近年来,在水溶性共轭聚合物(CPs)方面的研究备受瞩目,由于它包含了聚合物共轭主链良好的光电性质的同时还兼具了良好的水溶性,因此在光电功能信息器件中有着特殊的应用,并显著地推动了包括生物传感、电致发光器件、太阳电池和场效应晶体管等有机光电子材料及其器件的发展.本文对近几年来水溶性CPs的合成及其应用进展做出简要的总结,...     

Cover Picture: Intramolecular Dimers: A New Design Strategy for Fluorescence‐Quenched Probes (Chem. Eur. J. 15/2003)

The cover picture shows a 96‐well plate illuminated by a UV lamp and viewed through a cutoff filter. All wells contain a Cy3.5‐BHQ1 (reporter quencher) dual‐labeled oligonucleotide probe in buffer. Complementary sequence was added to three columns. The probe is virtually nonfluorescent due to static quenching through formation of an intramolecular dimer. Addition of complement separates the dye and quencher, causing fluorescence. Intramolecular dimers as a new design strategy for fluorescence‐quenched probes is discussed by M. K. Johansson and R. M. Cook in the Concepts article on p. 3466 ff.     

Synthesis and analytical properties of micrometric biosensing lipobeads

This paper describes the preparation for the first time of lipobead-based micrometric fluorescence biosensors and the optimization of their analytical properties. The study focused on the well-established urea biosensors as a model system. Fluorescence-sensing lipobeads were prepared by coating carboxyl-functionalized silica microspheres with phospholipids. The enzyme urease and the pH indicator fluorescein-5-thiosemicarbazide were then attached covalently to the phospholipid membrane of the lipobeads. Urease converts urea to ammonia, which results in a pH increase in the analyte solution and to a urea concentration-dependent increase in the fluorescence intensity of the sensing lipobeads. Previous fluorescence-sensing lipobeads were synthesized by coating polystyrene particles with a phospholipid membrane. The membrane was physically attached to the particles and the fluorophores were entrapped in the membrane. In this study, we prepared improved fluorescence-sensing lipobeads by utilizing covalent chemistry to bind the phospholipid membrane to the silica particles and the fluorophores to the membrane. This led to improvement in the stability of the newly developed urea-sensing lipobeads compared to previously developed miniaturized fluorescence biosensors.     

纳米金表面修饰与表面等离子体共振传感器的相互作用研究

为了分析纳米金表面修饰对表面等离子体共振(SPR)的放大作用,以及其对传感器本身的影响,首先,基于色散介质的吸收理论,通过建立波长型SPR生物传感器四层膜结构的数学模型,理论分析了传感器表面所吸附纳米金对传感器的影响:纳米金的表面修饰,改变了表面等离子体传感器中棱镜表面各介质层内电磁场的能量分布,削弱了金属膜在共振吸收中的作用,从而使SPR曲线的半波宽度增加,最小反射系数增大,金膜的最优膜厚度也随之改变.其次,通过不同厚度的金膜外吸附纳米金的对比试验,验证了此理论.金膜厚45nm、表面修饰10nm纳米金颗 关键词： 表面等离子体共振 生物传感器 纳米金 金属膜