Investigation of the interaction between acridine orange and bovine serum albumin

The results from the measurement of the fluorescence spectrum showing the binding characteristics of acridine orange (AO) and bovine serum albumin (BSA) are reported. It was found that the equilibrium constant k=4848.64 l mol⁻¹, and the number of binding sites n=0.82. Based on the mechanism of the Forster energy transference, the transfer efficiency of energy and distance between the acceptor AO and BSA were found. The interaction between AO and BSA have been verified as consistent with the static quenching procedure and the quenching mechanism is related to the energy transfer.     

各种离子对血卟啉与牛血清白蛋白相互结合反应的影响研究

应用荧光法研究了水溶液体系中血卟啉与牛血清白蛋白分了间相互结合反应，其结合常数ＫＡ＝２．２×１０＾４，结合位置数为１．依据Ｆｏｒｓｔｅｒ非辐射能量转移机理，探讨了血卟啉与牛血清白蛋白相互结合时，其给体－受体间距离和能量转移效率，进一步研究了各种离子与牛血清白蛋白的结合反应，其结合淬灭能力大小顺序为Ｃｒ（Ⅵ）〉Ｆｅ＾３＋〉Ｃｕ＾２＋〉ＮＯ２〉Ｂｒ＾－，并探讨不同阴，阳离子及血卟啉与牛血清白蛋白间相互     

Determination of trace levels of mercury in water samples based on room temperature phosphorescence energy transfer

A novel method has been developed for the sensitive determination of mercury in aqueous media by room temperature phosphorescence (RTP). The measurement principle is based on the energy transfer (ET) from a phosphor molecule (acting as a donor) to a Hg-sensitive dye (acceptor). To our acknowledgment this is the first RTP method for mercury measurement developed so far. α-Bromonaphthalene (BrN) was selected as the phosphorescent donor molecule (BrN can produce significant RTP emission in aqueous media in a β-cyclodextrin rigid microenvironment without deoxygenation).The absorption spectrum of the complex formed between mercury and the dithizone dye possesses a desirable spectral overlap with the RTP emission spectrum of the donor (BrN), giving rise to a nonradiative ET from the phosphor molecules to the mercury complex. An increase in the concentration of Hg(II) causes an increase on the concentration of the dithizone complex (acceptor) with the subsequent increase of the absorbance and, therefore, resulting in a decrease of the RTP emission. Both, RTP intensities and triplet lifetimes of the BrN decreased with increases on the Hg(II) concentration.Possible interferences present in natural waters, including different cations and anions, which could affect the analytical response, were evaluated and the analytical performance characteristics investigated. The use of phosphorescence measurements (low background noise signals) resulted in an improvement on the sensitivity of the Hg(II) detection higher than five times as compared to the molecular absorption spectrophotometric method for Hg(II) detection based on dithizone as Hg-indicator. A detection limit (D.L.) of 14 ng ml⁻¹ of Hg(II) was obtained by RTP with a precision of ±4.8% for five replicates of 300 ng ml⁻¹ of Hg(II). The usefulness of the method was successfully evaluated by the determination of Hg(II) in spiked natural water samples.     

High-Resolution Infrared Synchrotron Investigation of (HCN)2 and a Semi-Experimental Determination of the Dissociation Energy D0

The high-resolution infrared absorption spectrum of the donor bending fundamental band ν of the homodimer (HCN)₂ has been collected by long-path static gas-phase Fourier transform spectroscopy at 207 K employing the highly brilliant 2.75 GeV electron storage ring source at Synchrotron SOLEIL. The rovibrational structure of the ν transition has the typical appearance of a perpendicular type band associated with a Σ–Π transition for a linear polyatomic molecule. The total number of 100 assigned transitions are fitted employing a standard semi-rigid linear molecule Hamiltonian, providing the band origin ν₀ of 779.05182(50) cm⁻¹ together with spectroscopic parameters for the degenerate excited state. This band origin, blue-shifted by 67.15 cm⁻¹ relative to the HCN monomer, provides the final significant contribution to the change of intra-molecular vibrational zero-point energy upon HCN dimerization. The combination with the vibrational zero-point energy contribution determined recently for the class of large-amplitude inter-molecular fundamental transitions then enables a complete determination of the total change of vibrational zero-point energy of 3.35±0.30 kJ mol⁻¹. The new spectroscopic findings together with previously reported benchmark CCSDT(Q)/CBS electronic energies [Hoobler et al. ChemPhysChem. 19 , 3257–3265 (2018)] provide the best semi-experimental estimate of 16.48±0.30 kJ mol⁻¹ for the dissociation energy D₀ of this prototypical homodimer.     

Calculation of the thermondynamic values of alkylthiazoles from the Kovats retention indices on the basis of the non-linear additivity principle

Summary Retention indices and their energy equivalents were determined for ten alkylthiazoles analysed on the stationary phases Apiezon L, Triton X-305 and Carbowax 1000. It was found that the energy contributions of identical alkyl groups to the partial molar free energy of sorption of a molecule are not equivalent. Smaller energy contribututions are due to the α-position of alkyl groups with respect to the heteroatoms in the thiazole ring (α-effect). This effect was most pronounced for 2-alkylthiazoles. Higher sorption energies were found for dimethylthiazoles with two methylgroups in a vicinal position. The retention indices were predicted for ten other di- and tri-substituted methyl, ethyl and propyl thiazoles on the basis of the so-called α- and ortho-effects. The predicted values are in good agreement with the experimentally determined values. Dedicated to Prof. Dr. E. sz. Kováts (Ecole Polytechnique Fédérale de Lausanne) on the occasion of his sixtieth birthday.     

天线若丹明染料分子内能量与电荷传递的研究

染料三重态在染料激光的应用中起着重要作用，尤其是三重态一Z重态（T－T）吸收常常会造成谐振腔损耗＊．为了减少由基态吸收而造成的话振腔损耗，带有紫外吸收天线分子的三发色团染料已在研究问，2，5·二苯基螨喳（PP0）在紫外区（如308删）有很强的吸收，PPO－rhod．系列染料（见图1）在紫外区的吸收就很强，由PPO到若丹明母体的单线态一单线态（S功能量传递使这类三发色团染料具有较大的荧光量子效率，较小的基态重复吸收耗能卜，司．然而，在610N640nm区域中这些天线若丹明染料的激光输出效率远远小于若丹明Rh630＊，其原因正是…     

内含式化合物X@B12P12的结构与稳定性研究

采用B3LYP/6-31G*方法，对内含式化合物X@B₁₂P₁₂(X=Li^0/+、Na^0/+、K^0/+、Be^0/2+、Mg^0/2+、Ca^0/2+、H和He)的不同对称性构型进行了计算，讨论其最稳定构型的几何参数、布居分析、偶极矩、电离势、包含能、振动频率、能隙和自旋密度． 发现在X@B₁₂P₁₂化合物中，客体X=Li、Na^0/+、K^0/+、Mg^0/2+、Ca^0/2+和He处在偏离笼的中心0.006 nm的半径内． Be²⁺沿着C₃轴偏离中心点0.279 nm． 在Be@B₁₂P₁₂和H@B₁₂P₁₂的基态结构中，Be和H与笼上的B原子成键． 除Li@B₁₂P₁₂、 Be²⁺@B₁₂P₁₂和He@ B₁₂P₁₂外， 其余结构为C_s对称稳定构型．     

基本参数法在能量色散X射线荧光分析金属样品中的应用

在X射线荧光分析中,基本参数法是校正元素间吸收增强效应的数学方法之一。我们使用带有不锈钢窗的~(241)Am59.6keVγ射线作为激发源,用Si(Li)半导体探测器和多道脉冲幅度分析器作为实验手段。采用无标样法,即绝对测量法,因而具有更大的广泛性和实用性。实验中,我们除了考虑元素间的二次、三次荧光吸收增强作用外,对样品中元素     

Properties of strong hydrogen-bonded systems: The formation of hydrogen-bonded ion pair in amine·HCL systems

The hydrogen-bonded complexes between CH₃NH₂ and (CH₃)₂NH with HCl have been studied by the ab initio molecular orbital method using the 4–31G basis set. Calculations show that the proton potential curve has a single minimum near to the nitrogen atom in both complexes. This means that the proton has been transferred from HCl to the amine. ΔE and the dipole moment of the complexes studied are as follows: ?18.2 kcal mol^?1, 10.3 D for methylamine ·HCl, and ?21.7 kcal mol^?1 11.1 D for the corresponding dimethylamine complex. Other properties of the hydrogen-bonded ion pairs are discussed.