Colorimetric and fluorescence dual-mode detection of arsenic（v） based on cobalt oxyhydroxide nanoplatesEI北大核心CSCD

The oxidase-like activity of cobalt oxyhydroxide （CoOOH） nanosheets is inhibited by arsenic （V） in the system, thereby attenuating the oxidation of p-phenylenediamine （PPD） by CoOOH nanosheets, and changing the absorbance of the solution. Based on this principle, and the fluorescence inner filter effect （IFE） of p-phenylenediamine oxide （oxPPD） on the dye acridine orange （AO）, a colorimetric and fluorescence dualmode method for the quantitative detection of As（V） was established. The variation of absorbance and fluorescence intensity of As（V） with different concentrations was investigated, and the effects of the concentrations of CoOOH nanosheet and PPD, pH of buffer solution, and temperature of reaction were optimized. The results showed that under the optimized conditions, the concentration of As（Ⅴ） had a good linear relationship with the corresponding signal intensity, and the detection limits of colorimetric and fluorescence method were 5 and 10 nmol/L, respectively. The method has been used for the determination of arsenic（V） in lake water located the city center. © 2023, Youke Publishing Co.,Ltd. All rights reserved.     

A simple and highly repeatable colorimetric toxicity assay method using 2,6-dichlorophenolindophenol as the redox color indicator and whole eukaryote cells

A simple and highly reproducible toxicity assay method was studied by employing 2,6-dichlorophenolindophenol (DCIP) as a redox color indicator, baker’s yeast Saccharomyces cerevisiae, and a thermostable three-consecutive-stir unit. The absorbance of DCIP was decreased by increasing the metabolism activity of S. cerevisiae to intake glucose as an organic substance. By optimizing the measurement conditions, we obtained highly sensitive responses to glucose between 0.75 and 30 mg/L (eight points, n = 3) with an incubation time of the reaction mixture of 10 min at 30 °C. An excellent value of 1.15% was obtained as the average of the repeatability from eight points. Next, for the characterization of this method, we investigated the influence on the colorimetric response of dissolved substances, such as inorganic ions and surfactants, in natural water. Furthermore, the colorimetric responses to several toxicants were examined using Cu²⁺, Mn²⁺, Zn²⁺, Cr³⁺, and Fe³⁺ as heavy-metal ions and simazine as an agricultural chemical. As a result, notable colorimetric responses were obtained for Cu²⁺ and Mn²⁺ at several concentrations, and the results were compared with those obtained using river water as a real sample. In the stability test, responses to 30 mg/L glucose were obtained for 28 days when the yeast cell suspension was stored at 4 °C (response reduction, 43.9%; average of the relative standard deviation for nine testing days, 22.7%; average of repeatability, 1.01%). Figure Graphical image of the colorimetric toxicity assay     

彩色计算全息颜色匹配的研究

基于色度学原理,研究了彩色计算全息颜色的匹配问题,给出了电子显示色系下颜色量与计算全息物光波振幅之间的等色传递关系.首先,讨论了电子显示色系和彩色计算全息色系之间的颜色传递问题,并且给出了这两个色系下颜色量的转换方法.其次,以彩色全息再现像与原始计算目标颜色一致为目的,分析了彩色计算全息色系下的颜色量和与其对应物光波振幅的关系,并得出了颜色量和物光波振幅间应满足的一般方程.最后,本文选取PAL制式彩色显示色系下的彩色目标为全息图计算物体进行了彩色全息显示实验,结果表明本文所述彩色计算全息颜色匹配方法是有效的.本文的研究工作将为彩色计算全息显示的实用化奠定一定的理论基础和技术依据.     

Asensitive,Selective, and Rapid Colorimetric Method to Determine Cyanide Contamination in Containers,Capsules, and Liquids

Abstract

A new formulation of the Konig colorimetric method for cyanide is selective, has increased stability, and is sensitive to a few ppb. The formulation can be placed as a strip on the safety seals of bottles to indicate tampering, placed in a syringe and used to sample head space gas in commercial products or liquids to test for tampering, or placed in the desiccant container in bottles of capsules or tablets. Reaction with head space gas requires just a few seconds, and less than an hour is required if the cyanide must first permeate through capsules. Liquids also can be either drawn directly into the syringe, or the cyanide evolved by adding an Alka-seltzer tablet to purge the gas. Reactions are immediate. Hydrogen sulfide and ammonia which interfere with several other cyanide tests, do not interfere with this formulation. A mixture of N-chlorosuccinimide and succinimide oxidizes CN^- to CN⁺. The cyanogen ion selectively reacts with 4-phenylpyridine to form a dialdehyde, which is then coupled with either barbituric acid (red rapidly turning to blue) or 3-methyl-l-phenyl-2-pyrazolin-5-one (purple slowly turning to green black).     

Colorimetric Determination of Some N-Substituted Phenothiazine Derivatives Using 2-Iodoxybenzoate

Abstract

A new, sensitive colorimetric method has been described for the quantitative determination of six N-substituted phenothiazines. the method depends on the formation of stable phenothiazine free radical by the use of 2-iodoxybenzoate as chromogenic reagent. the drug in 50% sulphuric acid is treated with reagent (5×10^?4 M). the produced red or purple color possesses characteristic absorption maximum for each of the drugs tested. Beer's law is obeyed over the concentration range 2-30 μg.ml^?1 with apparent molar absorptivities ranging from 9.4-12.4×10^?3 for the studied phenothiazine derivatives. the average % recovery is 99.5±0.96 - 101.6±1.01. the method was applied successfully to the microdetermination of promazine. HCl, chlorpromazine, HCl, promethazine. HCl, perphenazine, levomepromazine HCl and mesoridazine besylate either in pure form or incorporated in their representative pharmaceutical preparations. the stoichiometry of the reaction was assumed and a reaction mechanism was suggested. the precision of the assay was comparable with the official ones.     

Spectrophotometric and Spectrofluorimetric Methods for the Determination of Terazosin in Dosage Forms

Five simple and accurate methods are presented for the determination of terazosin (TZ) in tablets. These methods are based on: the direct measurements of the first and second derivative spectra of samples (A), the reaction of TZ with chloranil (CH) in aqueous solution of pH 9 to give an intense yellow color measured at 340 nm (B), the reaction of the drug with mercurochrome (MER) in aqueous alkaline medium to give an intense red color measured at 543 nm (C), the formation of an ion-pair salt between the drug and bromocresol purple (BCP) with subsequent absorbance measurements at 412 nm (D), and a sensitive fluorimetric method (E). The latter method was extended to determine TZ in presence of its degradation products.     

适配体纳米金比色分析技术研究进展

核酸适配体作为一种生物识别分子,其本质是一段单链DNA或RNA,折叠形成特定的二级、三级构象后与靶标分子以高亲和力高特异性结合。纳米金具有强烈的粒子间距光学效应,其在分散状态下呈红色,发生凝聚后变为蓝色。适配体可以通过共价偶联或静电吸附与纳米金结合,通过结合靶标来控制纳米金的颜色变化,从而提供了一种简单、快速、灵敏、可以肉眼观察的快速分析技术。综述了适配体纳米金比色分析技术的研究进展,通过对不同方法比较和讨论,提出了未来的研究方向。     

光波导共振技术结合比色法检测葡萄糖溶液的浓度

利用比色法结合双面金属包覆波导构建了一种高灵敏度的光波导振荡场传感器. 将葡萄糖溶液与氧化酶试剂混合, 使葡萄糖溶液显色并注入波导结构, 采用波长为650 nm的激光入射于波导表面, 经角度扫描产生衰减全反射(ATR)吸收峰, 通过ATR吸收峰的极小值来检测葡萄糖溶液的浓度变化. 实验结果表明, 该技术对葡萄糖溶液浓度的分辨率达到111 nmol/mL, 其灵敏度高于分光光度计.     

蒽酮比色法测定马铃薯淀粉深加工工艺废液总糖含量的研究

研究了葡萄糖、果糖、蔗糖、马铃薯淀粉标准液、马铃薯粉渣和马铃薯淀粉深加工工艺废液,确定了蒽酮比色法测定马铃薯淀粉深加工废液总糖含量的最佳实验条件。在马铃薯淀粉深加工废液中加入盐酸,在105℃消煮120min,用活性碳脱色,过滤,滤液加入蒽酮,沸水浴中加热12min,显色体系在620nm处的吸光度达到最佳值,糖的浓度在1—100μg/mL范围内符合比耳定律,线性相关系数为0.9997。用所拟方法对凉山洲盐源世富农业科技公司马铃薯淀粉深加工工艺废液总糖进行测定,相对误差在5%以内,加标回收率在96.42%—103.16%之间,结果满意。     

一种基于色度学的RGBW信号映射算法评价方法

随着对高亮度、低功耗绿色显示技术的不断追求,RGBW显示技术成为了国内外的热门研究方向,已有多种RGBW显示器在市场上开始推广。由于将传统显示器rgb信号转换到RGBW显示器RGBW信号的映射算法不仅需要保留初始颜色,且需要兼容具有不同子像素布局方式的RGBW显示器,因此,信号映射算法成为RGBW显示的关键。在介绍现有的五种信号映射算法的基础上,分析了RGBW显示器中新增的白色子像素对显示颜色的作用方式以及现有四种RGBW子像素布局方式对映射算法显示效果的影响;提出了优秀映射算法应满足的三点要求,并据此推导出优秀映射算法的普适性等式及衡量映射算法亮度提升能力的亮度因子;针对五种映射算法的模拟实验表明：满足普适性等式的映射算法能更好地保留初始颜色的色调和饱和度,亮度因子可有效表征算法对亮度的提升能力。该模型不仅可用于评价现有映射算法,而且可为新型映射算法的研究提供理论指导,使之兼容于具有不同硬件参数和子像素布局的显示器,有助于RGBW显示技术的普及推广。