呼气中痕量挥发性有机物的质子转移反应质谱在线检测研究

通过对自主研制的大气成分在线检测质子转移反应质谱的进样管路系统进行改造,建立了可在线检测呼气中痕量挥发性有机物的质子转移反应质谱装置。通过对呼气进样系统的旁路流量控制,实现对进样速度的调控,既可提高进样速度,以满足实时监测呼气中指定成分浓度变化;也可适时关闭旁路,以降低进样速度,从而对呼气成分进行全谱分析,避免采样袋采样和浓缩的复杂程序和潜在干扰。以作者呼出气体作为研究对象,对装置性能进行测试,结果表明:装置最快响应时间可达1s,对呼气中丙酮的探测灵敏度高达每10-9(V/V)浓度的信号强度为14.6counts/s,多次呼气测量重复性好,有望广泛应用于呼气疾病诊断研究。     

A novel approach for the detection of DNA using immobilized peptide nucleic acid (PNA) probes and signal enhancement by real-time immuno-polymerase chain reaction (RT-iPCR)

A new approach for the detection of DNA target molecules is described, using capture probes and subsequent signal enhancement by a uniform polymerase chain reaction (PCR). Peptide nucleic acid probes were immobilized in real-time PCR-compatible microtiter plates. After hybridization of biotinylated DNA targets, detection was performed by real-time immuno-PCR, a method formerly used for protein detection. We demonstrate the feasibility of this strategy for the qualitative detection of DNA oligonucleotides with a detection limit (LOD) of 6 attomol. Furthermore, the method was applied to PCR-amplified samples from genetically modified maize DNA (Mon810). A 483-bp DNA fragment was detected in mixture with 99.9% of noncomplementary DNA with a sensitivity down to the level of attomole. Figure       

凸面光栅成像光谱仪图像数据实时压缩技术

凸面光栅成像光谱仪图像数据量巨大,给数据的传输和存储带来了压力,因此要对图像数据进行实时压缩。首先根据成像原理对图像数据的特点进行了分析,得出去除空间相关性和谱间相关性的压缩途径;然后,进行了压缩算法分析,提出了在谱间进行一阶线性预测、谱内进行JPEG2000压缩的三维压缩方案;最后,设计了基于FPGA+ADV212的实时压缩系统,其中FPGA用于逻辑控制和预测算法实现,ADV212用于JPEG2000压缩。分析结果表明:该系统具备无损和有损压缩能力,能够实现图像数据实时压缩。     

Optimal real-time estimation in diffusion tensor imaging

Diffusion tensor imaging (DTI) constitutes the most used paradigm among the diffusion-weighted magnetic resonance imaging (DW-MRI) techniques due to its simplicity and application potential. Recently, real-time estimation in DW-MRI has deserved special attention, with several proposals aiming at the estimation of meaningful diffusion parameters during the repetition time of the acquisition sequence. Specifically focusing on DTI, the underlying model of the noise present in the acquired data is not taken into account, leading to a suboptimal estimation of the diffusion tensor. In this paper, we propose an optimal real-time estimation framework for DTI reconstruction in single-coil acquisitions. By including an online estimation of the time-changing noise variance associated to the acquisition process, the proposed method achieves the sequential best linear unbiased estimator. Results on both synthetic and real data show that our method outperforms those so far proposed, reaching the best performance of the existing proposals by processing a substantially lower number of diffusion images.     

一种光电自动监测的摩擦试验机

在摩擦学的研究中,许多摩擦学试验要求使用摩擦磨损试验机作为主要的测试和观察手段.本文介绍了一种光电自动检测的摩擦试验机,简述了该试验机的原理、构成、功能及配套软件的设计.该试验机以光电自动检测、CCD动态观测记录、计算机实时处理为主要特征,对摩擦过程的各种参数进行测量,观测记录摩擦图象,数据和图象送入计算机计算、记录和处理.