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41.
A laser induced breakdown spectrometry hyphenated with on-line continuous flow hydride generation sample introduction system, HG-LIBS, has been used for the determination of arsenic, antimony, lead and germanium in aqueous environments. Optimum chemical and instrumental parameters governing chemical hydride generation, laser plasma formation and detection were investigated for each element under argon and nitrogen atmosphere. Arsenic, antimony and germanium have presented strong enhancement in signal strength under argon atmosphere while lead has shown no sensitivity to ambient gas type. Detection limits of 1.1 mg L−1, 1.0 mg L−1, 1.3 mg L−1 and 0.2 mg L−1 were obtained for As, Sb, Pb and Ge, respectively. Up to 77 times enhancement in detection limit of Pb were obtained, compared to the result obtained from the direct analysis of liquids by LIBS. Applicability of the technique to real water samples was tested through spiking experiments and recoveries higher than 80% were obtained. Results demonstrate that, HG-LIBS approach is suitable for quantitative analysis of toxic elements and sufficiently fast for real time continuous monitoring in aqueous environments. 相似文献
42.
将金纳米粒子(AuNPs)标记的大肠杆菌O157∶H7(E.coli O157∶H7)的多克隆抗体(PAb)作为二抗,采用氨基偶联法将PAb固定在传感器表面作为一抗,通过三明治方法用双通道表面等离子体子共振(SPR)传感器对E.coli O157∶H7进行检测,并与SPR直接法检测进行了比较.结果表明,直接法的检出限为103cfu/mL,线性范围为103~109cfu/mL;AuNPs增强三明治法的检出限为10 cfu/mL,线性范围为10~1010cfu/mL,灵敏度比直接法提高了100倍,且具有更宽的检测范围.本方法不仅检测时间短,而且具有良好的选择性和重现性. 相似文献
43.
为了抑制蛟龙号载人潜水器水声通信受母船色噪声干扰的影响,本文提出了一种统计匹配滤波器在色噪声环境下使用的新方法,它通过将接收信号映射到一个生成子空间中进行信号检测,可提高输出水声通信信号的信噪比。受客观条件限制,向阳红09船——蛟龙号载人潜水器的试验母船噪声环境比较恶劣,对水声通信产生了较强的干扰。如何在向阳红09船的这种强、色噪声环境下,提升水声同步信号的检测能力,对提高水声通信的可靠性是非常有帮助的。实际采集试验数据分析结果验证了该检测算法比传统匹配滤波器有更强的信号检测能力。 相似文献
44.
45.
生物体内的细胞通常会分泌各种各样的蛋白质,这些蛋白质在生物体中发挥着重要作用,尤其是可被用于诊断各种疾病的发生和发展。多肽具有良好选择性、空间适应能力和识别灵活的特点,可与不同类型的蛋白分子形成非共价键,用于蛋白质的生物检测。将多肽与电化学生物传感器结合用于蛋白质的广谱检测具有良好的发展前景。本文介绍了多肽修饰的电化学传感器在不同蛋白质检测方面的研究进展,分析了待测蛋白质的不同对多肽修饰的电化学传感器分类的影响及其优缺点,提出了基于多肽的电化学传感器在不同蛋白质检测中存在的问题,并展望了其未来发展。 相似文献
46.
A label-free DNA-based electrochemical biosensor owning high sensitivity and selectivity has been established for detecting bisphenol A in a wide range of applications. Coupling the high electrochemical performance of graphene oxide-thionine-Au nanomaterial with the specific binding capacity of the aptamers to BPA, the monitoring of trace amount of BPA was realized, the detection limit was 3.3 pg ⋅ mL−1 with strong anti-interference. Besides, using molecular docking, it was found that BPA binds to the bases DC-49, DC-51, DG-52, DG-53 and DA-63 on the aptamer via hydrogen bonding and π-π stacking interactions. Finally, the biosensor had been successfully applied in different real samples. 相似文献
47.
《Journal of Saudi Chemical Society》2022,26(5):101531
We used SERS with silver nanoparticles (AgNPs) as the active substrate to develop a, simple, quick, and accurate method for the detection and characterization SARS-CoV-2 without the need for RNA isolation and purification. Inactivated SARS-CoV-2 was used. The SERS signals were more than 105 times enhanced than the normal Raman (NR) spectra. The SERS spectra of SARS-CoV-2 fingerprint revealed pronounced intensity signals of nucleic acids; aromatic amino acid side chains: 1007 cm?1 (Phe marker), 1095 cm?1 (CN and PO2? markers), 1580 cm?1 (Tyr, Trp markers). Vibrations of the protein main chain: 1144 cm?1 (CN and NH2 markers), 1221 cm?1 (CN and NH markers), 1270 cm?1 (NH2 marker), 1453 cm?1 (CHCH2 marker). All of these biomolecules could be adsorbed on the AgNPs surface's dense hot patches. The intensity of the SERS band varied with the concentration of SARS-CoV-2, with a virus detection limit of less than 103 vp/mL and RSDs of 20 %. 相似文献
48.
Hydrogen sulfide (H2S), is proposed as a cytoprotectant and gasotransmitter, involving in many physiological processes and regulating of some diseases. In addition, H2S is a small molecular with a minimum of steric hindrance compared with other reactive sulfur species. In physiological atmosphere, H2S is mainly existent in HS−, which has a strong nucleophilicity and reducing potency. It also can precipitate with some metal ions forming metallic sulfides with high precipitation coefficient. In recent years, the researchers have a desire to develop methods to achieve real-time detection of H2S in vivo, further understanding the physiology and pathology of H2S. In this minireview, we summarize recent progress for detecting of H2S in brain or cell and briefly expound the principle of methods with the comparison of the different methods between performance and temporal resolution. 相似文献
49.
《Arabian Journal of Chemistry》2022,15(11):104199
The harmful effects of pesticide residues are a threat to our health. Therefore, the current study aimed to validate a simple method for the determination of pesticide residues in commonly consumed fruits and vegetables from Al-Rass, Al-Qassim region, Saudi Arabia. A total of 1430 samples were collected from a local market and then analyzed for monitoring of 49 pesticide residues. A quick, easy, cheap, effective, rugged, and safe (QuEChERS) multi-residue extraction method followed by gas chromatography equipped with triple-quadrupole mass spectrometry (GC–MS/MS) was successfully implemented. This 17-min-run analytical method detects and quantifies pesticide residues with acceptable validation performance parameters in terms of sensitivity, selectivity, linearity, the limit of quantification, accuracy, and precision. The linear range of the calibration curves ranged from 10 to 300 µg/L, all the pesticide LODs ranged from 0.0005 to 0.0024 mg/kg, and the pesticide LOQs ranged from 0.0011 to 0.0047 mg/kg. The recovery values at the three fortification levels ranged from 78 % to 107 %, and the precision values (expressed as RSD%) were less than 20 % for all of the investigated analytes. The results showed that 138 (9.65 %) of the analyzed samples were contaminated with pesticide residues, 40 (2.80 %) of the analyzed samples exceeded the maximum residue limit (MRL) of the European Commission regulations (EC) for pesticides residues, 98 (6.85 %) of the analyzed samples were contaminated with residues below the MRL, and 1292 (90.35 %) of the analyzed samples were pesticide residue-free. Coriander contained the highest percentage (46.88 %) of pesticide residues, particularly tetradifon that representing 18.75 % noncompliance with the MRL, followed by parsley, with 20.59 % pesticide residues (10.29 % non-compliance). Multiple pesticide residues were observed most frequently in tomatoes and dates which were contaminated with buprofezin and ethion respectively. 相似文献
50.
K. Alagumalai R. Shanmugam T.-W. Chen S.-M. Chen M. Balamurugan S.S. Choi M.A. Ali A.M. Al-Mohaimeed C.-H. Fan 《Materials Today Chemistry》2022
In this work, we developed a perovskite structured samarium cobalt oxide nanoparticles (SmCoO3 NPs) with the aid of the co-precipitation method. The rare earth metal (Sm) and cobalt oxide combined to form a perovskite lattice structure. One-pot route synthesized SmCoO3 NPs were scrutinized successfully through various physicochemical techniques. Concerning its effective thermal stability and electrical properties, the synthesized SmCoO3 NPs have been effectively implemented in the electrochemical evaluation of promethazine hydrochloride (PHY) using cyclic voltammetry. The electrochemical detection of PHY was performed through SmCoO3 NPs-modified glassy carbon electrode (GCE) and unmodified GCE. The electron transfer kinetics, effect of scan rate, the influence of pH, electroactive surface area, selectivity, and sensitivity have been studied. The electron charge transfer rate (Rct) and electrolyte resistance (Rs) were calculated to be 105.59 (Ω) and 150 (Ω) in the ferricyanide probe, indicating great facilitation of the electron transfer between PHY and SmCoO3 NPs deposited on the electrode surface. Further, the optimized SmCoO3-modified GCE exemplifies excellent selectivity, storage stability, reproducibility, repeatability, detection limit (5 nM), sensitivity (0.594 μA μM?1 cm?2), and wide consecutive linear ranges, respectively. Besides, the proposed method has been effectively employed for the detection of PHY in the various real samples which reveals good recoveries of 95.40–99.17%. 相似文献