喜树叶中喜树碱含量的高效液相色谱分析

建立了一种简便、快速、准确的喜树叶片中喜树碱含量的高效液相色谱分析方法 ;采用英国HPLCTechnology公司的TechsphereODS柱 (25cm×4.6mmID ,5μm) ,流动相乙腈 -水 (体积比4∶6) ,流速1.0mL·min -1,检测波长254nm ,柱温25℃ ,进样量10μL ;样品制备方法以61 % (φ)乙醇为溶剂 ,50℃下超声提取喜树叶粉10min;HPLC法测定喜树碱的含量 ;该法的RSD为2.5% (n=6) ,平均回收率为96 %。     

On complexity of Minimum Leaf Out-Branching problem

Given a digraph D, the Minimum Leaf Out-Branching problem (MinLOB) is the problem of finding in D an out-branching with the minimum possible number of leaves, i.e., vertices of out-degree zero. Gutin, Razgon and Kim [G. Gutin, I. Razgon, E.J. Kim, Minimum leaf out-branching problems, in: Proc. 4th International Conference on Algorithmic Aspects in Information and Management, AAIM’08, in: Lect. Notes Comput. Sci., vol. 5034 2008, pp. 235-246] proved that MinLOB is polynomial time solvable for acyclic digraphs which are exactly the digraphs of directed path-width (DAG-width, directed tree-width, respectively) 0. We investigate how much one can extend this polynomiality result. We prove that already for digraphs of directed path-width (directed tree-width, DAG-width, respectively) 1, MinLOB is NP-hard. On the other hand, we show that for digraphs of restricted directed tree-width (directed path-width, DAG-width, respectively) and a fixed integer k, the problem of checking whether there is an out-branching with at most k leaves is polynomial time solvable.     

氢化物发生-原子荧光光谱法测定城市脐橙树叶中铅

研究氢化物发生-原子荧光光谱法测定城市脐橙树叶中铅的适宜条件,并对脐橙树叶中铅量进行了测定.研究表明:叶中的含铅量与车流量呈正相关.方法的线性范围为0.00～1.50 mg·L-1,相关系数为0.997 8,检出限为0.19μg·L-1,回收率在93.2%～105.0%之间.     

Plumbagin,a Naturally Occurring Naphthoquinone: Its Isolation,Spectrophotometric Determination in Roots,Stems, and Leaves in Plumbago Europaea L.

A new method for isolation and spectrophotometric determination of plumbagin is presented. Plumbagin was isolated by thin layer chromatography (TLC) and column chromatography (CC) techniques, as an orange tinged yellow long crystalline substances. Plumbagin exhibits two absrop-tion maxima at 410 and 510 nm. Stability of the color, pK_a value, and the effect of pH were studied. Beer's law is obeyed over the range 0.9-45 ppm. The method is applied to the determination of plumbagin in roots, stems, and leaves of Plumbago europaea L. plant.     

Kinetic-coulometric determination of mercury in biological samples

A simple approximate calculation method is given which permits determina-tion of the maximal scan rates of potential allowable for distortion-free recording of current-voltage curves with X-Y recorders. For the calculations only the response time of the recorder, the wave shape and the scan rate of potential need be known. Stationary mercury electrodes and rapid polarography with a dropping mercury electrode at controlled drop times were examined. Electroanalytical implications are discussed, with particular emphasis on the rapid a.c. polarographic method with short controlled drop times and on stationary-electrode fundamental and second harmonic a.c. voltammetry. Theoretically and experimentally it has been shown that an X-Y recorder with 0.5–1.0-s response time can be used for scan rates up to about (50/nt') mV s^-1 with a.c. techniques and about (100/nt') mV s^-1 with d.c. polarography (t'= response time of recorder, n = number of electrons).     

NMR spectra of meta-difluorobenzene in nematic solvents: molecular geometry and anisotropy of the indirect fluorine-fluorine coupling

Nuclear magnetic resonance spectra of 1,3-difluorobenzene in thermotropic and lyotropic nematic solvents have been measured and interpreted. The geometrical data calculated from the dipolar couplings are compared with those obtained from microwave spectra and from a recent electron diffraction experiment. Evidence has been found of a small anisotropy in the indirect fluorine-fluorine coupling.     

An optimized method to extract poplar leaf proteins for two‐dimensional gel electrophoresis guided by analysis of polysaccharides and phenolic compounds

Commonly used methods for protein extraction from plant leaves, such as extraction with phenol or a combination of trichloroacetic acid and acetone, were ineffective for four tested cultivars of poplar. Moreover, multiple protocols for 2DE of the extracted proteins gave different results when protein profiles of relatively closely related plants were compared. Given that polycyclic compounds strongly hinder 2DE, we analyzed the impact of polyphenols and polysaccharides present in the plant tissues used for protein extraction, on the quality of 2DE protein profiles. Analysis of content of polyphenols and polysaccharides in leaves of poplar cultivars showed that even small differences in concentrations of analyzed metabolites accompany large differences between poplar cultivars when considering the susceptibility of samples to protein extraction for 2DE. High‐quality 2DE results were correlated with decreased amounts of polyphenols. Additional analysis using MS/MS suggested that only levels of total phenolics affected the results of 2DE. Soluble total nonstructural carbohydrates also had a negative effect, but the level of starch was not important. Finally, we present an optimized method for extraction of proteins from poplar leaves, which enables reliable comparative analysis of four different poplar cultivars, that is, “Eridano,” “Villafranca,” “NE‐42,” and “Luisa Avanzo,” which have not yet been used for the proteomic studies.     

山楂叶中总黄酮含量的测定

用索氏提取法提取山楂叶中黄酮化合物.以芦丁为标准品,对其进行络合显色,在最大吸收峰500nm处测定提取物中总黄酮的含量.山楂叶中总黄酮的含量以芦丁计算为9.16%,平均加样回收率为98.53%.方法简便、快速、重现性好,可作为检测山楂叶中黄酮含量的一种手段.     

Symmetries in trees and parking functions

We investigate a particular symmetry in labeled trees first discovered by Gessel, which can be stated as follows: In the set of rooted labeled trees on n+1 vertices rooted at the smallest vertex, the number of trees with a descents and b+1 leaves equals the number of trees with b descents and a+1 leaves. We present two new ways to prove the symmetry resulting from decompositions of trees, which lead to three different bijections from trees to trees in which leaves and descents are swapped. We also interpret the symmetry in terms of parking functions: the number of parking functions on [n] with a descents and b unfavorable spaces (defined in this paper) equals the number of parking functions on [n] with b descents and a unfavorable spaces. We conclude with a generalization of these results to binary trees.RésuméNous étudions une symétrie particulière dans les arbres étiquetés, découverte par Gessel, qu'on peut énoncer comme suit: Dans l'ensemble des arbres étiquetés pointés avec n+1 sommets, pointés au sommet minimum, le nombre d'arbres avec a descentes et b+1 feuilles égale le nombre d'arbres avec b descentes et a+1 feuilles. Nous présentons deux nouvelles démonstrations de la symétrie, qui resultent des décompositions des arbres; à partir des décompositions, nous obtenons trois bijections des arbres sur les arbres qui échangent les feuilles et les descentes. De plus, nous interprétons la symétrie en termes des “fonctions de stationnement” (parking functions): le nombre des fonctions de stationnement avec a descentes et b positions défavorables (définies dans cette article) égale le nombre de fonctions de stationnement avec b positions défavorable et a descentes. Nous donnons aussi une généralisation de ces resultats aux arbres binaires.     

小叶香樟新鲜树叶挥发性成分分析

采用水蒸汽蒸馏和乙醚萃取收集小叶香樟新鲜树叶挥发油,经气质联用分析鉴定出49个化合物,占峰面积的95.9%,挥发油得率为1.73%。主要化合物的类型为单萜(86.4%)、倍半萜(10.0%)、芳香族(1.41%)和脂肪族(1.13%)。其中质量分数最高的为L-芳樟醇(45.9%),其次为樟脑(28.5%)和橙花叔醇(4.66%)。