全文获取类型
收费全文 | 569篇 |
免费 | 11篇 |
国内免费 | 28篇 |
专业分类
化学 | 383篇 |
力学 | 19篇 |
综合类 | 1篇 |
数学 | 33篇 |
物理学 | 172篇 |
出版年
2024年 | 1篇 |
2023年 | 6篇 |
2022年 | 8篇 |
2021年 | 11篇 |
2020年 | 8篇 |
2019年 | 6篇 |
2018年 | 7篇 |
2017年 | 11篇 |
2016年 | 14篇 |
2015年 | 16篇 |
2014年 | 21篇 |
2013年 | 29篇 |
2012年 | 42篇 |
2011年 | 32篇 |
2010年 | 30篇 |
2009年 | 30篇 |
2008年 | 25篇 |
2007年 | 40篇 |
2006年 | 24篇 |
2005年 | 25篇 |
2004年 | 24篇 |
2003年 | 18篇 |
2002年 | 15篇 |
2001年 | 11篇 |
2000年 | 18篇 |
1999年 | 17篇 |
1998年 | 14篇 |
1997年 | 12篇 |
1996年 | 12篇 |
1995年 | 13篇 |
1994年 | 16篇 |
1993年 | 4篇 |
1992年 | 7篇 |
1991年 | 6篇 |
1990年 | 9篇 |
1989年 | 5篇 |
1988年 | 2篇 |
1987年 | 4篇 |
1986年 | 1篇 |
1984年 | 3篇 |
1983年 | 1篇 |
1982年 | 5篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1978年 | 1篇 |
1971年 | 1篇 |
1968年 | 1篇 |
排序方式: 共有608条查询结果,搜索用时 359 毫秒
51.
Sivaiah Areti Jayshree K. Khedkar Sateesh Bandaru Rohit Teotia Jayesh Bellare Chebrolu Pulla Rao 《Analytica chimica acta》2015
A coumarine–imino–C2-glucosyl conjugate (L) was synthesized and characterized. The conjugate L is found to recognize Cu2+ in aqueous HEPES buffer by exhibiting a 95% fluorescence quenching in pH range 7–10 even in the presence of several biologically and ecologically relevant metal ions. Fluorescence on–off behavior has been clearly demonstrated on the basis of the binding variability of Cu2+ to L. The binding has been elicited through the changes observed in fluorescence, absorption, ESI-MS and 1H NMR titrations. All the other thirteen metal ions studied did not show any change in the fluorescence emission. These ions do not interfere with the recognition of Cu2+ by L. The structural features of [CuL]2 complex in both the isomeric forms were established by DFT computational calculations. The utility of L has been demonstrated by showing its sensitivity toward Cu2+ on a thin layer of silica gel. The L gives sensitive fluorescence signals for Cu2+ even in blood serum and exhibits appropriate fluorescence responses in living cells. 相似文献
52.
Polymer inclusion membranes (PIMs) have several important features, i.e., PIMs are dry and non-porous membranes, which can be prepared ahead of use and stored without noticeable deterioration in extraction performance. In this contribution, in-line coupling of microextractions across PIMs to a separation method for clinical purposes was demonstrated for the first time. Formate (the major metabolite in methanol poisoning) was determined in undiluted human serum and whole blood by capillary zone electrophoresis (CZE) with simultaneous capacitively coupled contactless conductivity detection (C4D) and UV–Vis detection. A purpose-made microextraction device with PIM was coupled to a commercial CZE instrument in order to ensure complete automation of the entire analytical procedure, i.e., of formate extraction, injection, CZE separation and quantification. PIMs for formate extractions consisted of 60% (w/w) cellulose triacetate as base polymer and 40% (w/w) Aliquat™ 336 as anion carrier. The method was characterized by good repeatability of peak areas (≤7.0%) and migration times (≤0.8%) and by good linearity of calibration curves (r2 = 0.993–0.999). Limits of detection in various matrices ranged from 15 to 54 μM for C4D and from 200 to 635 μM for UV–Vis detection and were sufficiently low to clearly distinguish between endogenous and toxic levels of formate in healthy and methanol intoxicated individuals. In addition, PIMs proved that they may act as phase interfaces with excellent long-term stability since once prepared, they retained their extractions properties for, at least, two months of storage. 相似文献
53.
54.
Papoutsis I Nikolaou P Athanaselis S Alevizopoulos G Pistos C Paraskevopoulou C Spiliopoulou C 《Journal of separation science》2011,34(21):3037-3042
Sildenafil (SDL) is a phosphodiesterase type 5 inhibitor and it is approved for the treatment of erectile dysfunction and pulmonary hypertension. SDL is extensively metabolized to its pharmacologically active metabolite, desmethyl‐sildenafil (DSDL). A sensitive and specific GC/MS method for the determination of SDL and DSDL in whole blood was developed and validated to support therapeutic drug monitoring of SDL patients. The combination of solid‐phase extraction with derivatization using BSTFA with 1% TMCS in acetonitrile efficiently reduced matrix effect and improved sensitivity of the method. In this assay, protriptyline was used as internal standard for both analytes. The LODs were 1.50 and 5.00 ng/mL for SDL and DSDL, respectively, whereas the respective LOQs were 5.00 and 15.0 ng/mL. The calibration curves were linear up to 500.0 ng/mL (SDL: R2 0.992, DSDL: R2 0.990). Absolute recovery values for both analytes ranged from 83.1 to 93.2%. Within‐ and between‐batch accuracy was less than 11.8 and 10.2%, respectively, whereas within‐ and between‐batch precision was less than 8.1 and 10.8%, correspondingly. The developed method is suitable for the determination of SDL and DSDL concentrations in blood samples obtained from patients under Viagra® treatment, for pharmacokinetic studies or for the investigation of related forensic cases. 相似文献
55.
The aim of this paper is to describe and discuss the results of numerical comparative study performed in order to demonstrate and quantify some of the most relevant non-Newtonian characteristics of blood flow in medium-sized blood vessels, namely its shear-thinning and viscoelastic behavior.The models studied in this work are the classical Newtonian and Oldroyd-B models, as well as their generalized (shear-thinning) modifications. Numerical tests are performed on three-dimensional geometries, namely an idealized axisymmetric stenosis and a realistic stenosed carotid bifurcation reconstructed from medical images. The numerical solution of the system of governing equations is obtained by a finite-volume method on a structured grid. Model sensitivity tests are achieved with respect to the characteristic flow rate to evaluate its impact on the observed non-Newtonian effects. 相似文献
56.
In the present paper, blood flow through a tapered artery with a stenosis is analyzed, assuming the flow is steady and blood is treated as non-Newtonian power law fluid model. Exact solution has been evaluated for velocity, resistance impedance, wall shear stress and shearing stress at the stenosis throat. The graphical results of different types of tapered arteries (i.e. converging tapering, diverging tapering, non-tapered artery) have been examined for different parameters of interest. Some special cases of the problem are also presented. 相似文献
57.
The new bioaffinity membranes comprise mammalian blood and tissue cells immobilized in the polymer matrix. The method of immobilization does not assume the retention of physiological and enzymatic activity of immobilized cells, but it ensures the safety of cellular membrane receptors that are used as specific ligands. Macroporous polymer carriers based on polyacrylonitrile maintain the accessibility of the cellular receptors for all blood plasma components including immunoglobulins and viral particles. The sorption capacity of membranes with respect to model substances in a batchwise technique is evaluated. Although the results are of a preliminary nature, the membranes may be used in crossflow modules for selective blood plasma correction of endogenous substances. 相似文献
58.
文章报道用铂作基体改进剂直接测定全血中铅的石墨炉原子吸收光谱法。同时应用衬钽技术,大大延长了石墨管的使用寿命。该方法准确、简便快速,灵敏度为1.74μg·L~(-1),回收率为96%~103%,变异系数小于5%。 相似文献
59.
傅立时变换中红外光谱法用于非损伤性血糖测定的研究 总被引:1,自引:0,他引:1
本文首次使用中红外光纤对人体血糖进行了非损伤性测定,实验显示,人手指的中红外光谱1123cm-1谱带的相对强度与人的血糖浓度同步改变,表明葡萄糖特征谱带的相对强度确可作为人血糖值的一个数量指标。这给红外光纤在生物医学领域中的应用展示了光明的前景。 相似文献
60.
用无火焰原子吸收光谱测定家兔从肝动脉注射顺氯氨铂前后的血药浓度及在体内分布情况。 相似文献