微波消解ICP-MS测定新疆一枝蒿中15种微量元素

采用微波消解样品,电感耦合等离子体质谱法（ICP-MS）测定新疆一枝蒿中Li、B、V、Cr、Mn、Fe、Co、Ni、Cu、Zn、Mo、Cd、Tl、Pb、Bi15种微量元素.结果表明：这15种元素的加标回收率为80.06%～111.00%,相对标准偏差小于5.00%.本方法简便、快速、灵敏度高、准确,可同时测定多种元素.     

大高良姜与节鞭山姜油细胞原位拉曼光谱研究

为避免复杂的样品的制备及提取过程,最大限度避免精油活性成分变化,在常温下,用拉曼光谱原位分析大高良姜与节鞭山姜油细胞中精油。两种样品的拉曼光谱非常相似,较强峰出现在1636/1643、1605/1607、1291/1293、1199/1204、1168/1168cm~(-1),中等强度的峰出现在1585/-、1439/1437、1308/-、907/908、856/856、797/788、731/732、632/633cm~(-1)。说明两者的挥发油主要成分相同。与1’一乙酰氧基胡椒酚乙酸酯(1’-acetoxychavico lacetate)拉曼光谱主要的16条谱线比较,在节鞭山姜油细胞拉曼谱的18条谱峰中有15条与之对应,而对大高良姜而言19条谱线中,有14条与之对应。用密度泛函理论计算了ACA的拉曼光谱,并对谱线进行了初步的归属。     

A new benzofuran from Artemisia halodendron Turcz. ex Bess.

A new benzofuran, methyl (2S,2″S,3′E)-[2-(1″-acetoxypropan-2-yl)-2,3-dihydrobenzofuran-5-yl]acrylate (1), and 13 known compounds (2–14) were isolated from an ethanol extract of Artemisia halodendron Turcz. ex Bess. The chemical structures of these compounds were determined by 1D and 2D NMR (¹H-¹H COSY, HMBC, HMQC and NOESY) and HR-ESI-MS spectra, and results were compared with data from the literature. The effects of compounds 1–14 were measured on NF-κB activation, with compounds 2 and 3 exhibiting inhibitory activities against TNF-α-induced NF-κB reporter gene expression in HeLa cells from 10 to 100 μM.     

野生藜蒿中的微量元素的质谱分析

研究应用电感耦合等离子体串联质谱(ICP-MS/MS)技术建立准确测定野生藜蒿中12个微量元素的分析方法。采用MS/MS模式,以O₂为反应气,元素As和Se与O₂发生质量转移反应生成AsO和SeO,通过测定AsO和SeO消除质谱干扰;以NH₃/He为反应气,元素Cr,Mn,Fe,Co,Ni,Cu,Zn与NH₃/He发生质量转移反应形成团簇离子,通过测定团簇离子消除质谱干扰。元素Cd,Hg和Pb采用标准模式进行测定。12个待测元素的校准曲线在0～200 μg·L-1范围内具有良好的线性关系,各元素的检出限为0.64～49.61 ng·L-1。通过测定国家标准物质芹菜(GBW10048),采用t检验法进行差异性检验表明,测定值与标准值之间无显著性差异,验证了方法的准确可靠性。各元素的加标回收率在92.0%～106.1%之间,相对标准偏差(RSD)为1.6%～4.9%。分析了来自国内不同地区的4个野生藜蒿样品,结果显示,不同来源样品中12个元素的含量各有差异,Mn,Fe,Zn的含量远高于其余九种元素,毒理性元素As,Cd,Hg和Pb的含量处于极低水平。方法能准确测定野生藜蒿中的多种微量元素,为野生藜蒿的食用营养和安全提供科学的理论依据。     

9β-hydroxy-11β(H),13-dihyhdrosantamarine, a new eudesmanolide fromArtemisia leucotricha

A new sesquiterpene lactone, 9-hydroxy-11(H),13-dihydrosantamarine, and known terpenes are isolated from the underground part ofArtemisia leucotricha Krasch ex Ladyg (Asteraceae). The structure is determined by spectral and x-ray structural analysis.Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 230–232, May–June, 2000.     

Phenolic Compounds of Salix acutifolia Bark

Column chromatography over silica gel and polyamide was used to isolate 14 phenolic substances from bark of sharp-leaved willow (Salix acutifolia Willd.). These included naringenin, prunin, (-)-salipurposide, (+)-catechin, isosalipurposide, 6-coumarylisosalipurposide, isosalipurpol (flavonoids), triandrin, syringin (phenylpropanoids), and salicyl alcohol (saligenin) and its derivatives acylsaligenin, salicin, salicortin, and tremulacin. Nine of these were isolated for the first time from this species. They were identified using UV, IR, NMR, and mass spectra, chemical transformations, and comparison with authentic samples.     

火焰原子吸收法测定栽培柴胡中六种金属元素含量的研究

采用浓硝酸-高氯酸(V:V=4:1)常压微沸条件下消解柴胡样品,应用火焰原子吸收法测定栽培柴胡中的金属元素Ca,Mg,Fe,Cu,Zn,Pb的含量.研究了测定不同元素的仪器最佳工作条件及溶液介质对测定结果的影响,并做了方法的准确性和精密度考察.结果表明,栽培柴胡中Ca,Mg,Fe,Cu,Zn的含量分别为5 588.9,1 790.5,869.3,78.4,44.3μg·g-1,Pb未检出.方法的加标回收率为99.57%～102.10%,相对标准偏差(n=9)为0.18%～2.26%.测定方法简单易行,方便快捷,结果可靠.     

Potential Mechanisms Involved in the Protective Effect of Dicaffeoylquinic Acids from Artemisia annua L. Leaves against Diabetes and Its Complications

Diabetes mellitus is a chronic disease affecting the globe and its incidence is increasing pandemically. The use of plant-derived natural products for diabetes management is of great interest. Polar fraction of Artemisia annua L. leaves has shown antidiabetic activity in vivo. In the present study, three major compounds were isolated from this polar fraction; namely, 3,5-dicaffeoylquinic acid (1); 4,5-dicaffeoylquinic acid (2), and 3,4- dicaffeoylquinic acid methyl ester (3), using VLC-RP-18 and HPLC techniques. The potential protective effects of these compounds against diabetes and its complications were investigated by employing various in vitro enzyme inhibition assays. Furthermore, their antioxidant and wound healing effectiveness were evaluated. Results declared that these dicaffeoylquinic acids greatly inhibited DPPIV enzyme while moderately inhibited α-glucosidase enzyme, where compounds 1 and 3 displayed the most prominent effects. In addition, compound 3 showed pronounced inhibition of α-amylase enzyme. Moreover, these compounds markedly inhibited aldose reductase enzyme and exerted powerful antioxidant effects, among which compound 3 exhibited the highest activity implying a notable potentiality in impeding diabetes complications. Interestingly, compounds 2 and 3 moderately accelerated scratch wound healing. Our findings suggest that these dicaffeoylquinic acids can be promising therapeutic agents for managing diabetes and its complications.     

Four New Benzoylamide Derivatives Isolated from the Seeds of Lepidium apetalum Willd. and Ameliorated LPS-Induced NRK52e Cells via Nrf2/Keap1 Pathway

Four new benzoylamide derivatives, lepidiumamide B–E (1–4), were isolated from the seeds of Lepidium apetalum Willd. The structures were determined by a combination of MS and NMR analyses. All compounds were evaluated for their protective effects against NRK-52e cell injury induced by lipopolysaccharide (LPS) in vitro. These compounds showed significantly protective activity and ameliorated LPS-induced NRK52e cells via the Nrf2/Keap1 pathway. The discovery of these active compounds is important for the prevention and treatment of renalinjury.