Proof of a conjecture of Alan Hartman

A tree T is said to be bad, if it is the vertex‐disjoint union of two stars plus an edge joining the center of the first star to an end‐vertex of the second star. A tree T is good, if it is not bad. In this article, we prove a conjecture of Alan Hartman that, for any spanning tree T of K_2m, where m ≥ 4, there exists a (2m − 1)‐edge‐coloring of K_2m such that all the edges of T receive distinct colors if and only if T is good. © 1999 John Wiley & Sons, Inc. J Graph Theory 30: 7–17, 1999     

Time asymmetric quantum theory – II. Relativistic resonances from S‐matrix poles

Relativistic resonances and decaying states are described by representations of Poincaré transformations, similar to Wigner's definition of stable particles. To associate decaying state vectors to resonance poles of the S‐matrix, the conventional Hilbert space assumption (or asymptotic completeness) is replaced by a new hypothesis that associates different dense Hardy subspaces to the in‐ and out‐scattering states. Then one can separate the scattering amplitude into a background amplitude and one or several “relativistic Breit‐Wigner” amplitudes, which represent the resonances per se. These Breit‐Wigner amplitudes have a precisely defined lineshape and are associated to exponentially decaying Gamow vectors which furnish the irreducible representation spaces of causal Poincaré transformations into the forward light cone.     

Supereulerian regular matroids without small cocircuits

A cycle of a matroid is a disjoint union of circuits. A matroid is supereulerian if it contains a spanning cycle. To answer an open problem of Bauer in 1985, Catlin proved in [J. Graph Theory 12 (1988) 29–44] that for sufficiently large $n$, every 2-edge-connected simple graph $G$ with $\text{◂=▸}n=\mid V\left(G\right)\mid$ and minimum degree $\text{◂≥▸}\delta \left(G\right)\ge \frac{n}{5}$ is supereulerian. In [Eur. J. Combinatorics, 33 (2012), 1765–1776], it is shown that for any connected simple regular matroid $M$, if every cocircuit $D$ of $M$ satisfies $\text{◂≥▸}\mid D\mid \ge \max \left\{\frac{\text{◂−▸}r\left(M\right)-5}{5},6\right\}$, then $M$ is supereulerian. We prove the following. (i) Let $M$ be a connected simple regular matroid. If every cocircuit $D$ of $M$ satisfies $\text{◂≥▸}\mid D\mid \ge \max \left\{\frac{\text{◂+▸}r\left(M\right)+1}{10},9\right\}$, then $M$ is supereulerian. (ii) For any real number $c$ with , there exists an integer $f\left(c\right)$ such that if every cocircuit $D$ of a connected simple cographic matroid $M$ satisfies $\mid D\mid \ge \text{◂lim▸}\max \text{◂{}▸}\{c\text{◂()▸}\left(r\left(M\right)+1\right),f\left(c\right)\}$, then $M$ is supereulerian.     

Rapid isolation and quantification of extracellular vesicles from suspension-adapted human embryonic kidney cells using capillary-channeled polymer fiber spin-down tips

Exosomes, a subset of extracellular vesicles (EVs, 30–200-nm diameter), serve as biomolecular snapshots of their cell of origin and vehicles for intercellular communication, playing roles in biological processes, including homeostasis maintenance and immune modulation. The large-scale processing of exosomes for use as therapeutic vectors has been proposed, but these applications are limited by impure, low-yield recoveries from cell culture milieu (CCM). Current isolation methods are also limited by tedious and laborious workflows, especially toward an isolation of EVs from CCM for therapeutic applications. Employed is a rapid (<10 min) EV isolation method on a capillary-channeled polymer fiber spin-down tip format. EVs are isolated from the CCM of suspension-adapted human embryonic kidney cells (HEK293), one of the candidate cell lines for commercial EV production. This batch solid-phase extraction technique allows 10¹² EVs to be obtained from only 100-µl aliquots of milieu, processed using a benchtop centrifuge. The tip-isolated EVs were characterized using transmission electron microscopy, multi-angle light scattering, absorbance quantification, an enzyme-linked immunosorbent assay to tetraspanin marker proteins, and a protein purity assay. It is believed that the demonstrated approach has immediate relevance in research and analytical laboratories, with opportunities for production-level scale-up projected.