回传波矩阵法在杆系结构静力分析中的应用

回传波矩阵法最初是由Pao等人分析二维框架结构动力响应时提出的。对于三维杆系结构的静力分析,为了确定结构的位移和内力,先要建立传递分配矩阵和载荷源向量,这可通过列出所有节点的静力平衡方程和位移协调方程来实现。同时,通过分析每根杆近端位移和远端位移的关系,建立结构的回传波矩阵（重分配矩阵）。在此基础上求解线性方程组,就可以得到结构的位移和内力。本文推导了空间杆系结构的有关矩阵方程式,并给出了一固定梁的两端弯矩求解算例。     

Diatomaceous Earth for Arthropod Pest Control: Back to the Future

Nowadays, we are tackling various issues related to the overuse of synthetic insecticides. Growing concerns about biodiversity, animal and human welfare, and food security are pushing agriculture toward a more sustainable approach, and research is moving in this direction, looking for environmentally friendly alternatives to be adopted in Integrated Pest Management (IPM) protocols. In this regard, inert dusts, especially diatomaceous earths (DEs), hold a significant promise to prevent and control a wide range of arthropod pests. DEs are a type of naturally occurring soft siliceous sedimentary rock, consisting of the fossilized exoskeleton of unicellular algae, which are called diatoms. Mainly adopted for the control of stored product pests, DEs have found also their use against some household insects living in a dry environment, such as bed bugs, or insects of agricultural interest. In this article, we reported a comprehensive review of the use of DEs against different arthropod pest taxa, such as Acarina, Blattodea, Coleoptera, Diptera, Hemiptera, Hymenoptera, Ixodida, Lepidoptera, when applied either alone or in combination with other techniques. The mechanisms of action of DEs, their real-world applications, and challenges related to their adoption in IPM programs are critically reported.     

Time asymmetric quantum theory – II. Relativistic resonances from S‐matrix poles

Relativistic resonances and decaying states are described by representations of Poincaré transformations, similar to Wigner's definition of stable particles. To associate decaying state vectors to resonance poles of the S‐matrix, the conventional Hilbert space assumption (or asymptotic completeness) is replaced by a new hypothesis that associates different dense Hardy subspaces to the in‐ and out‐scattering states. Then one can separate the scattering amplitude into a background amplitude and one or several “relativistic Breit‐Wigner” amplitudes, which represent the resonances per se. These Breit‐Wigner amplitudes have a precisely defined lineshape and are associated to exponentially decaying Gamow vectors which furnish the irreducible representation spaces of causal Poincaré transformations into the forward light cone.     

Rapid isolation and quantification of extracellular vesicles from suspension-adapted human embryonic kidney cells using capillary-channeled polymer fiber spin-down tips

Exosomes, a subset of extracellular vesicles (EVs, 30–200-nm diameter), serve as biomolecular snapshots of their cell of origin and vehicles for intercellular communication, playing roles in biological processes, including homeostasis maintenance and immune modulation. The large-scale processing of exosomes for use as therapeutic vectors has been proposed, but these applications are limited by impure, low-yield recoveries from cell culture milieu (CCM). Current isolation methods are also limited by tedious and laborious workflows, especially toward an isolation of EVs from CCM for therapeutic applications. Employed is a rapid (<10 min) EV isolation method on a capillary-channeled polymer fiber spin-down tip format. EVs are isolated from the CCM of suspension-adapted human embryonic kidney cells (HEK293), one of the candidate cell lines for commercial EV production. This batch solid-phase extraction technique allows 10¹² EVs to be obtained from only 100-µl aliquots of milieu, processed using a benchtop centrifuge. The tip-isolated EVs were characterized using transmission electron microscopy, multi-angle light scattering, absorbance quantification, an enzyme-linked immunosorbent assay to tetraspanin marker proteins, and a protein purity assay. It is believed that the demonstrated approach has immediate relevance in research and analytical laboratories, with opportunities for production-level scale-up projected.