A fluorescent probe for Hg2+sensing in solutions and living cells with a wide working pH range简

In this paper, 2-carboxybenzaldehyde rhodamine B thiohydrazine(1) was synthesized and developed as a fluorescent probe to recognize Hg2+in DMF/H2 O(1:9, v/v) solution with high selectivity. The probe can be applied to the quanti?cation of Hg2+with a linear concentration range covering from 1.0×10-7mol/L to 1.0×10-5mol/L(R2= 0.9985) and a detection limit of 4.2×10-8mol/L. The experiment results show that the response of probe 1 to Hg2+is pH-independent in a wide range from 4.0 to 9.0. Moreover, the probe 1 exhibits excellent selectivity toward Hg2+over other common metal cations. Most importantly, the probe can be employed to monitor Hg2+in living cells using fluorescent imaging technique with satisfied results.     

Ceric ammonium nitrate supported HY-zeolite：An efficient catalyst for the synthesis of 1, 8-dioxo-octahydroxanthenes

Ceric ammonium nitrate （CAN） supported HY-zeolite has been used as an efficient catalyst for the one- pot synthesis of 1,8-dioxo-octahydroxanthenes from the readily available 1,3-diketone and aromatic aldehydes under solvent-free conditions. The present methodology is cost-effective in addition to other advantages like high yields of products in shorter reaction time and simple workup procedure without the use of any injurious solvents.     

关于太阳能热水器的物理研究

太阳能热水器具有安全、节能、经济、环保等优点，可以改善和提高人们的居住条件与生活水平．为了让大家了解太阳能热水器，使太阳能热水器走人普通百姓生活中，特对太阳能热水器的有关问题进行了研究．     

盘声珠韵——图说中国算盘发展简史

<正>五、通商惠工——清代民国时期算盘的普及清代珠算广泛使用。上至天文历算,下至农田水利;大到国家经济发展,小到百姓家庭生活,都离不开算盘。珠算在促进经济发展、推动社会进步方面,发挥了极为重要的作用。民国时期,新工业兴办,     

组合发电仪的制作

仪器应用生活中常见的材料,仅仅围绕物理学科知识,通过巧妙设计,组装出一系列发电机。旨在让学生明白各种发电机的工作原理,以及能量之间是如何相互转化的,培养学生节约能源,保护环境利用清洁能源的意识。此外本仪器制作简单,便于学生亲手制作,锻炼动手能力的同时,又可提高学习兴趣。     

多美“概率”

概率密切联系生活与现实世界，使数学问题情景新颖别致，丰富多彩．纵观近几年各省、市高考和模拟题，概率好题不胜枚举，俯拾皆是，它们像一颗颗璀璨的珍珠在数学题海中闪闪发光．本文特精选举例予以分类解析，旨在探究题型规律，揭示解题方法、提供解题规律，供读者参考．     

善用“错误”实验 拓展学生才智

物理来源于生活，又反馈给生活，同时促进社会生产力水平的发展．     

平行线与截线——主角和配角

一般地，平行线是用“不相交”这种否定方式定义的，其中包含了对空间的想象．而在实际生活中，理解平行线是无限延伸的，无论怎样延伸也不会相交是一个理解的难点．正是由于截线的出现，提供了同位角、内错角，和同旁内角的运算关系．对平面内两条直线的位置关系，可以从定性的讨论而到定量的刻画，体现了数学对客观世界定性把握和定量刻画．     

Investigation on activating individual living monocytic U937 cell by interleukin-6 using Raman tweezers

We investigate the activation of living monocytic U937 cells induced by interleukin-6 （IL-6） at the single cell level. We employ home-built Raman tweezers to measure the Raman spectra of living U937 cells with and without IL-6 at the single cell level. Raman peaks of amide III, amide I, DNA backbone, as well as guanine and adenine in U937 cells, change at 1312, 1652, 1090, and 1576 cm ？1 , respectively, shortly after IL-6 is added in the medium. The change is a dynamic temporal process. In the activation process of U937 cells induced by IL-6, the protein signals recover in 20 min, while the nucleic acid signals continue to increase for 20 min. The results reveal that the biochemical cascades of activation in signal transduction induced by IL-6 can be investigated in situ at the single cell level.     

讨厌的辅导班

刺龙虾是生活在海底的巨型龙虾，比我们常见的龙虾大得多，有些可重达10千克。每到秋季，它们就要结束悠闲的生活．开始漫长的“辅导班”学习——沿着海底艰苦跋涉几百千米，去寻找新鲜的食物。为了安全，它们要排成单行紧紧跟着前面的龙虾。并且穿越50千米都不休息。真是辛苦呀!