Stochastic mechano-chemical kinetics of molecular motors: A multidisciplinary enterprise from a physicist’s perspective

A molecular motor is made of either a single macromolecule or a macromolecular complex. Just like their macroscopic counterparts, molecular motors “transduce” input energy into mechanical work. All the nano-motors considered here operate under isothermal conditions far from equilibrium. Moreover, one of the possible mechanisms of energy transduction, called Brownian ratchet, does not even have any macroscopic counterpart. But, molecular motor is not synonymous with Brownian ratchet; a large number of molecular motors execute a noisy power stroke, rather than operating as Brownian ratchet. We review not only the structural design and stochastic kinetics of individual single motors, but also their coordination, cooperation and competition as well as the assembly of multi-module motors in various intracellular kinetic processes. Although all the motors considered here execute mechanical movements, efficiency and power output are not necessarily good measures of performance of some motors. Among the intracellular nano-motors, we consider the porters, sliders and rowers, pistons and hooks, exporters, importers, packers and movers as well as those that also synthesize, manipulate and degrade “macromolecules of life”. We review mostly the quantitative models for the kinetics of these motors. We also describe several of those motor-driven intracellular stochastic processes for which quantitative models are yet to be developed. In part I, we discuss mainly the methodology and the generic models of various important classes of molecular motors. In part II, we review many specific examples emphasizing the unity of the basic mechanisms as well as diversity of operations arising from the differences in their detailed structure and kinetics. Multi-disciplinary research is presented here from the perspective of physicists.     

Development of carbon-carbon nozzle extension for liquid fuel rocket motors

Successful experience of RSC “Energy” and SPA “Iskra” in the development of carbon-carbon extension for oxygen-kerosene liquid fuel rocket motor has been summarized. Methodological approach that served to completion of carbon-carbon extension development in full and at comparatively small expenses has been described. Results of practical application of carbon-carbon extension for liquid fuel rocket motor 11D58M have been presented within the framework of International Space Program “Sea Launch”.     

超导技术在飞轮储能系统中的应用及前景

阐述了飞轮储能装置原理和超导磁悬浮理论,并设计了超导飞轮储能系统的基本结构。采用超导磁悬浮轴承技术可以解决普通的飞轮储能系统由于有机械轴承摩擦产生的能量损耗,克服普通飞轮储能的低效、储能时间短等问题。最后简要介绍了超导飞轮储能技术的发展前景。     

Change in motor cortex activation for muscle release by motor learning

For central nervous system disorders'' rehabilitation, it is important to accurately understand motor control and implement an appropriate motor learning process to induce neuroplastic changes. The neurophysiological studies have revealed that neural control mechanisms are crucial during both the onset of muscular activities and muscle release after contraction. When performing various movements during daily activities, muscle relaxation control enables precise force output and timing control. Moreover, surround inhibition is a functional mechanism in the motor system. Surround inhibition of the motor system may be involved in the selective execution of desired movements. This review demonstrates cortical excitability resulting from motor learning, movement control mechanisms including muscle relaxation and the suppression of nontarget muscle groups, and the voluntary drive''s importance that is required for movement.     

基于工作电流分析的回转副摩擦状态识别实验研究

回转副摩擦状态的变化对机械设备的运行有着重要影响. 针对回转副摩擦状态的识别问题, 设计了以回转副工作电流为摩擦特征来源的实验方案. 通过采集摩擦过程的回转副驱动电机工作电流信号, 分析实验信号的频域和时频域特征, 建立工作电流的摩擦特征库, 用于训练多分类支持向量机分类器, 并在分类器上完成摩擦状态的识别. 实验结果表明, 经特征提取与降维处理后建立的多分类支持向量机分类器具有近90%的识别正确率, 所提出的实验方案有利于实现远距离信号的采集, 适用实际生产加工.     

含BN旋转轴的光驱动分子马达的理论设计和机理研究

依据B=N键与C=C键的电子结构相似性,以Feringa型二苯乙烯型光驱动分子马达（CC-stilbene）为母体,设计了含极性旋转轴的模型马达BN-stilbene.CASPT2//CASSCF计算结果表明,优化所得的BN-stilbene分子的基态存在四个与CC-stilbene马达结构相似、相对能量一致的螺旋异构体;B=N极性共价双键对BN-stilbene的基态和激发态电子结构有显著影响.对BN-stilbene模型马达的工作机理研究表明,极性旋转轴的引入使得BN-stilbene中S₁/S₀-CI与激发态中间体构型更加相似且能量更低,同时可增加旋转的驱动力,起到改进分子马达光异构化过程的单向性的目的.     

Dynamical behaviors of the chaotic Brushless DC motors model

This article is concerned with the boundedness solutions of the brushless DC motor system. For this system, the global attractive set and positively invariant set are obtained based on generalized Lyapunov function stability theory and the extremum principle of function. Furthermore, the rate of the trajectories is also obtained. Numerical simulations are presented to show the effectiveness of the proposed scheme. © 2014 Wiley Periodicals, Inc. Complexity 21: 79–85, 2016     

Monitoring ssDNA Binding to the DnaB Helicase from Helicobacter pylori by Solid‐State NMR Spectroscopy

DnaB helicases are bacterial, ATP‐driven enzymes that unwind double‐stranded DNA during DNA replication. Herein, we study the sequential binding of the “non‐hydrolysable” ATP analogue AMP‐PNP and of single‐stranded (ss) DNA to the dodecameric DnaB helicase from Helicobacter pylori using solid‐state NMR. Phosphorus cross‐polarization experiments monitor the binding of AMP‐PNP and DNA to the helicase. ¹³C chemical‐shift perturbations (CSPs) are used to detect conformational changes in the protein upon binding. The helicase switches upon AMP‐PNP addition into a conformation apt for ssDNA binding, and AMP‐PNP is hydrolyzed and released upon binding of ssDNA. Our study sheds light on the conformational changes which are triggered by the interaction with AMP‐PNP and are needed for ssDNA binding of H. pylori DnaB in vitro. They also demonstrate the level of detail solid‐state NMR can provide for the characterization of protein–DNA interactions and the interplay with ATP or its analogues.     

多模态控制在步进电机位置伺服系统中的应用研究

针对步进电机伺服系统的响应速度慢、稳态误差低的问题,提出了一种多模态控制策略。根据误差大小调节两种控制策略对被控对象的作用权值,使最终的控制量连续输出,实现控制策略无扰动、平滑的切换。半实物仿真实验结果表明,该控制策略较好地综合了模糊控制和模糊PID控制的优势,系统反应速度快,震荡小,抗干扰能力强,同时一定程度上改善了低频振荡和高频失步的问题,具有实际应用价值。     

以太阳为光源测量大气污染物研究中精确跟踪太阳的方法

本文给出了通过计算太阳在天空中移动的轨迹和利用在实验中所采集到的太阳光谱主动精确跟踪太阳的方法,并且通过在以太阳为光源测量大气中污染物浓度的实验研究中的实际应用,证明了该方法的可靠性和实用性.