High-Throughput Analysis of Sofalcone in Human Plasma by Use of Automated 96-Well Protein Precipitation and LC-MS-MS

A sensitive and selective method for the determination of sofalcone in human plasma was established by use of protein precipitation and liquid chromatography-tandem mass spectrometry. Plasma samples were transferred into 96-well plate using an automated sample handling system and spiked with 10 L of 2 g mL^–1 internal standard solution (d₃-sofalcone). 0.5 mL of acetonitrile was added to the 96-well plate and the plasma samples were then vortexed for 30 sec. After centrifugation, the supernatant was transferred into another 96-well plate and completely evaporated at 40 °C under a stream of nitrogen. The dry residue was reconstituted with mobile phase. All sample transfer and protein precipitation was automated through the application of both the PerkinElmer MultiPROBE II HT and TOMTEC Quadra 96 workstation. The limit of quantitation of sofalcone was 2 ng mL^–1 using a sample volume of 0.2 mL for the analysis. The reproducibility of the method was evaluated by analyzing five samples at nine quality control (QC) levels over the nominal concentration range from 2 ng mL^–1 to 1000 ng mL^–1. Validation of the method showed that the assay has good precision and accuracy. Sofalcone and internal standard produced a protonated precursor ion ([M+H]⁺) at m/z 451 and 454, and both gave a corresponding product ion at m/z 315. The high sample throughput of the method has been successfully applied to a pharmacokinetic study of sofalcone in human plasma.     

Evaluation of phenyldimethylethoxysilane treated high-performance thin-layer chromatographic plates. Application to analysis of flavonoids inScutellariae radix

Summary The retention and selectivity of flavonoids (baicalin, baicalein, wogonin, oroxylin A) inScutellariae radix have been studies by high-performance thin-layer chromatography on phenyldimethylethoxysilane-treated silica plates. The silica plates treated with phenyl groups were used for physical and chemical analysis. From elemental carbon analysis, the maximum number of bonded phenyl surface groups per gram was calculated to be 0.467×10²¹ (Oginal silica plate: Merck Art. 15109, Silica gel 100 F₂₅₄). With methanol-1/15 M phosphate buffer (pH 6.2) mixtures as mobile phase, baicalin, baicalein, wogonin, and oroxylin A inScutellariae radix were separated. It has been shown that phenyl-treated plates are more suitable for selective separation of baicalin, baicalein, wogonin, and oroxylin A than octadecyl-treated plates.     

Surface free energies of styrene-acrylic acid copolymer plates prepared in various frames

Styrene (St)-acrylic acid (AA) copolymer plates were synthesized in the frames made of glass, aluminum (Al) and poly(difluoroethylene) (PDFE). Surface properties of the prepared copolymer plates were characterized with regard to critical surface tension ( _C ), chemical components by IR analysis and surface energies (total energy, _S ; dispersion force components, _S ^d ; polar component, _S ^p ; hydrogen bonding component, _S ^h ) and the following results were obtained.The _C values of the copolymer plates increased with AA content and also depended on the sort of the used frame. The increasing order of the _C values of the copolymer plates corresponded to those of the used frames, namely, PDFE frame < Al frame < Glass frame.The prepared copolymer plates with low AA contents (ca. 10 mol%) were enriched in the AA moiety in the surface layer regardless of the kind of the used frame.The total and the individual components of the surface free energies of the copolymer plates were largely affected by the property of the used frame. The glass frame gave the plate with higher _S ^h values suggesting preferential orientation of the polar site of AA component.     

基于修正模态应变能指标的板结构损伤定位

为解决模态应变能方法识别中产生的"邻近效应"问题,提出基于修正模态应变能指标的板结构损伤定位方法.该方法首先利用邻近测点的应变能变化相对大小计算权重系数,再根据权重系数对测点相应区域的应变能进行重新分配.此外,通过定义的损伤辨识度指标研究噪声对损伤定位结果的影响.为验证本文所提方法的可行性和有效性,以一个四边简支板为数值算例.算例结果表明,本文方法对于点状、块状和带状损伤都可以实现准确定位,且具有良好的抗噪性.     

硬夹心矩形夹层板的整体稳定性分析

论文在Reissner型理论给出的位移模式基础上,修正其软夹心假设,考虑夹心层面内刚度,给出了硬夹心夹层板的几何方程、物理方程,建立了硬夹心夹层板结构在面内纵向载荷作用下的平衡微分方程,并对方程进行了简化,通过理论计算得到了四边简支条件下硬夹心矩形夹层板整体失稳临界载荷的解析解,并分别计算了夹心层材料的弹性模量E、厚度h、泊松比μc对硬夹心夹层板临界载荷的影响,结果证明,对于硬夹心夹层结构,夹心层面内刚度对硬夹心夹层板整体失稳临界载荷的影响较大,考虑其面内刚度是必要的.     

功能梯度简支矩形板的非线性动力响应

研究了功能梯度简支矩形板在横向简谐激励作用下的非线性动力响应问题.采用幂律分布规律描述功能梯度材料的等效材料参数,基于Galerkin法建立了系统广义坐标的常微分控制方程.利用平均法得到了系统的幅频响应特性,分析了功能梯度矩形薄板的非线性主共振特性.数值算例验证了平均化方法的正确性,揭示了功能梯度平板主共振响应中的多值性和跳跃现象;同时分析发现初始条件会改变功能梯度平板主共振的响应幅值.最后讨论了功能梯度材料的梯度指数对系统幅值响应的影响.     

正交偏振板对聚焦光斑偏振特性的影响

在间接驱动的惯性约束聚变（ICF）驱动器中,为控制靶腔入口及其附近光场的偏振特性及辐照均匀性,采用二维光谱角色散（2D-SSD）、随机相位板（RPP）和正交偏振板（OPCP）联用的束匀滑方案,数值模拟和理论分析了OPCP对焦面及离焦面处光场偏振特性以及强度均匀性的影响,并讨论了正交偏振板单元数的选取问题。结果表明,不同单元数正交偏振板对焦面及其附近光场偏振特性和强度均匀性的影响程度不同,且光场偏振特性对离焦并不敏感。此外,离焦会使强度均匀性变差,适当增加正交偏振板单元数可以在一定程度上减弱这种影响。     

核工程钢板混凝土墙防撞击贯穿实用计算方法

针对带对拉钢筋的双钢板混凝土墙,研究了双钢板混凝土墙防贯穿计算方法,建立了基于能量法的防贯穿计算公式。在已知弹体和钢板混凝土墙体材料与几何相关参数时,利用防贯穿实用计算公式可对带对拉钢筋的核工程双钢板混凝土墙体贯穿速度以及弹体剩余速度进行计算,避免了复杂的结构抗撞击反应动力时程数值分析。为验证公式的可靠性,将公式计算结果与已有刚性弹体撞击双钢板混凝土墙实验结果及其动力有限元计算结果进行对比,结果表明：防贯穿实用计算公式可以正确判断双钢板混凝土墙的贯穿状态,实用计算公式给出的弹体剩余速度与实验结果符合良好。为进一步验证公式的适用范围,将公式计算结果与共10个工况的飞机发动机撞击双钢板混凝土墙的有限元计算结果进行了对比分析,结果表明：除1个工况计算结果偏差略超10%外,其余工况的偏差均在10%以内,说明该计算方法合理可行。

     

“聚龙一号”装置上磁驱动铝飞片实验的数值模拟

采用二维磁驱动数值模拟程序MDSC2,对大电流脉冲装置聚龙一号上的151发次磁驱动370 m厚铝飞片实验、164发次磁驱动330 m厚铝飞片实验进行了数值模拟和分析。数值模拟表明：370 m厚铝飞片和330 m厚铝飞片的磁驱动过程中, VISAR测量的速度不是飞片自由面的速度,而是飞片中邻近自由面最近的固体反射面的速度。这是由于磁驱动飞片发射过程中,飞片自由面部分被烧蚀,密度低于固体密度状态,而飞片自由面和加载面中间的飞片还保持固体密度状态。VISAR测量的激光将穿过自由面的低于固体密度状态的飞片部分,到达飞片自由面最近的固体密度位置再反射回去,获得这一位置的飞片速度。数值模拟的飞片固体反射面速度历史和VISAR测量的速度历史相吻合。