High-speed counter-current chromatography assisted preparative isolation of phenolic compounds from the flowers of Chrysanthemum morifolium cv. Fubaiju

Chrysanthemum morifolium cv. Fubaiju is rich in phenolic compounds with various benefits such as anti-inflammatory, antioxidant, and cardiovascular protection. In this study, 12 phenolic compounds, including five flavonoid glycosides and seven quinic acid derivatives, were successfully separated from the flowers of Chrysanthemum morifolium cv. Fubaiju by high-speed counter-current chromatography and preparative high-performance liquid chromatography. Ethyl acetate-n-butanol–acetonitrile–water–acetic acid (5:0.5:2.5:5:0.25, v/v/v/v/v) was selected as solvent system to separate six fractions from the flowers of Chrysanthemum morifolium cv. Fubaiju, and 20% aqueous acetonitrile (containing 0.1% formic acid) was chosen to be the elution solvent in preparative high-performance liquid chromatography for purifying the fractions above. Luteolin-7-O-β-D-glucoside ( 1 ), luteolin-7-O-β-D-glucuronide ( 2 ), apigenin-7-O-β-D-glucoside ( 3 ), luteolin-7-O-β-D-rutinoside ( 4 ), diosmetin-7-O-β-D-glucoside ( 5 ), chlorogenic acid ( 6 ), 1,5-dicaffeoylquinic acid ( 7 ), 1,4-dicaffeoylquinic acid ( 8 ), 3,4-dicaffeoylquinic acid ( 9 ), 3,4-dicaffeoyl-epi-quinic acid ( 10 ), 3,5-dicaffeoylquinic acid ( 11 ), and 4,5-dicaffeoylquinic acid ( 12 ) were isolated with purities all above 95%, respectively. In addition, all isolates were evaluated for their protective effects on H₂O₂-induced oxidative damage in adult retinal pigment epithelial cells.     

An efficient strategy for large-scale preparation of low polarity gingerols directly from ginger crude extract by high-speed countercurrent chromatography with different rotation mode

This study presents an efficient strategy for large-scale preparation of low polarity gingerols directly from ginger crude extract by high-speed countercurrent chromatography with different rotation mode. The ultrasonic-assisted extraction conditions were optimized by response surface methodology and the results showed the major low polarity gingerols could be well enriched under the optimized extraction conditions. Then the crude extract without any pretreatment was directly separated by high-speed countercurrent chromatography with different rotation mode using n-hexane/ethyl acetate/methanol/water (6:4:6:4, v/v/v/v) as the solvent system. In about 400 min, five major gingerols including 150 mg of [6]-gingerol, 50 mg of [8]-gingerol, 20 mg of [6]-shogaol, 43 mg of [6]-dehydrogingerdione, and 40 mg of [10]-gingerol were obtained from 1.2 g of crude extract in a single run with repeated injection. Their structures were identified by ¹H-NMR spectroscopy.     

Rapid large-scale preparation of polysaccharides from jackfruit peel waste by high-speed countercurrent chromatography and their antioxidant and hypoglycemic activities

Polysaccharides with antioxidant and hypoglycemic activities were first isolated from jackfruit (Artocarpus heterophyllus Lam.) peel through the one-step high-speed countercurrent chromatography. The separation process was completed using the polymer two-phase aqueous system constituted by PEG1000-K₂HPO₄-KH₂PO₄-H₂O (0.8:1.25:1.25:6.5, w/w). For every separation process, two main polysaccharides, namely, fraction-1 and fraction-2 (165 and 225 mg, respectively) were obtained from a 2.0 g crude sample. As suggested by high-performance gel permeation chromatography, jackfruit peel polysaccharides had the mean molecular weight values of 113.3 and 174.3 kDa, separately. Physicochemical analysis suggested that two polysaccharides were dominant in galacturonic acid, galactose, rhamnose, arabinose, glucose, mannose, as well as fucose, which were highly esterified. Biological activity analysis showed that fraction-1 exhibited stronger antioxidant activity in vitro and hypoglycemic activity in streptozotocin-induced diabetic mice compared with fraction-2. The results suggest that polysaccharide fraction-1 may be developed as a potential functional food supplement.     

Linear Response of General Observables in Spiking Neuronal Network Models

We establish a general linear response relation for spiking neuronal networks, based on chains with unbounded memory. This relation allow us to predict the influence of a weak amplitude time dependent external stimuli on spatio-temporal spike correlations, from the spontaneous statistics (without stimulus) in a general context where the memory in spike dynamics can extend arbitrarily far in the past. Using this approach, we show how the linear response is explicitly related to the collective effect of the stimuli, intrinsic neuronal dynamics, and network connectivity on spike train statistics. We illustrate our results with numerical simulations performed over a discrete time integrate and fire model.     

Simulation of shape variation of projectile nose during high-speed penetration into concrete

A formula is developed to estimate the total mass loss of projectile, based on the assumptions that the peeling of molten surface layer in projectile nose is the primary cause of mass loss, and the frictional heat is totally absorbed by the projectile. Extrapolating this formula to predict the mass loss of local area of projectile, the receding displacement on projectile surface is obtained, which is vertical to the symmetry axis of projectile. Thereby, a finite difference method model is constructed to simulate the variation of projectile shape. The shape of residual projectile, depth of penetration of projectile and its mass loss obtained by calculation are found in good consistency with respective experimental data.     

A Sensitive Measurement of Maleic Hydrazide by a Differential Pulse Voltammetry Followed by a Cleansing Pulse Train

In this current project, we report the most sensitive scheme for the measurement of maleic hydrazide by a modified differential pulse voltammetry (DPV) which is composed of a conventional DPV followed with a cleansing pulse train. The absorbed oxidized species (1,2-pyridazine-3,6-dione) on the solid electrode surface cause subsequent mass transfer hindrance which can be removed easily by a cleansing pulse train of ±0.1 volts, 50 Hz for 5 minutes at the end of each voltammetric run. This scheme possesses the advantage of high sensitivity (submicro-molar range), which may be used in sensitive maleic hydrazide measurement or toxicity studies. This scheme offers a better tool to study a very low concentration of maleic hydrazide. A slope of 59.2 mV per decades from the potential versus pH plot was obtained. This result indicates that these are two electrons and two protons oxidation processes. The linear range of the calibration plot is up to 50 μM with a correlation coefficient of 0.9999. The sensitivity of the linear portion of the calibration plot yields a slope of 13.63 nA/μM with a detection limit of 20 nM (S/N = 3). The width of half peak width is 100 mV at the injection of 22 μM maleic hydrazide. Precision study possesses a relative standard deviation of 4.1% (n = 20).     

高速逆流色谱分离与鉴定鹿药中黄酮类化合物

采用高速逆流色谱(HSCCC)与其它色谱联用的方法分离纯化鹿药中的化学成分,得到5个黄酮类化合物: 5,7,3′,4′-四羟基-3-甲氧基-8-甲基黄酮(1)、 8-甲基木犀草素(2)、 3′-甲氧基木犀草素(3)、 木犀草素(4)和槲皮素(5),它们均为首次自该种及该属植物中分离得到.HSCCC以V(氯仿)∶ V(甲醇)∶ V(水)=4∶ 3∶ 2混合液为溶剂,上相为固定相,下相为流动相, 分离纯化得到3个分别含化合物1, 4和5的主要部分,经HPLC检验纯度分别为98.3%, 96.7%和95.3%;还有1个含有化合物2和3的较纯部分,通过Sephadex LH-20柱色谱,以V(甲醇)∶ V(水)=1∶ 1混合液洗脱将二者分离.通过理化性质及紫外、红外、质谱、核磁等波谱分析确定化合物结构.     

货运列车的优化编组调度方案与实现方法

针对2008年全国研究生数学建模竞赛C题"货运列车的编组调度问题",首先介绍了问题的背景和问题的构成,并提出了6个要解决的问题;然后概要地分析介绍了解决这6个具体问题的思想方法;接着给出了具体解决问题的实现方法、主要模型和求解思路;最后对参赛队的总体做法和存在问题情况做了较详细的分析,并就与这个题目有关的几个问题做了说明.     

一个基于张量火车分解的张量填充方法及在图像恢复中的应用

低秩张量填充在数据恢复中有广泛应用, 基于张量火车(TT) 分解的张量填充模型在彩色图像和视频以及互联网数据恢复中应用效果良好。本文提出一个基于三阶张量TT分解的填充模型。在模型中, 引入稀疏正则项与时空正则项, 分别刻画核张量的稀疏性和数据固有的块相似性。根据问题的结构特点, 引入辅助变量将原模型等价转化成可分离形式, 并采用临近交替极小化(PAM) 与交替方向乘子法(ADMM) 相结合的方法求解模型。数值实验表明, 两正则项的引入有利于提高数据恢复的稳定性和实际效果, 所提出方法优于其他方法。在采样率较低或图像出现结构性缺失时, 其方法效果较为显著。     

14N pulsed nuclear quadrupole resonance. 3. Effect of a pulse train. Optimal conditions for data averaging

The calculations developed in this paper aim at determining the optimal conditions of a NQR experiment when a transition is monitored by means of a pulse train with pulses of identical duration and signal acquisition after each pulse; coherences are assumed to vanish by effective transverse relaxation prior to every new pulse. These calculations demonstrate that, as in NMR, a steady state is effectively reached for any value of the recycle time. However, by contrast with NMR, it is shown that, for optimal data averaging under steady state conditions, the recycle time T can be kept as low as possible (the only limitation is the acquisition time). Nutation curves (signal amplitude versus pulse length) calculated in the steady state case are shown to depend strongly on the ratio T/T ₁ (T ₁: longitudinal relaxation time). The signal growth as a function of T/T ₁under averaging of the first transients has been evaluated as well as the number of pulses necessary for reaching a steady state.