Investigation of the Doughnut Effect in Cy3-Labeled Protein Microarrays using Scanning Near-Field Optical Microscope

We demonstrate the fluorescence mapping of protein microarrays by the technique of scanning near-field optical microscopy (SNOM) and confocal microscopy. Micron sized spots (300 μm) of human Immunoglobulin G (hIgG) protein with and without a Cy3 dye labeling have been fabricated on glass substrates by an immobilization method which makes use of calixcrown derivatives termed Prolinker. We have also tried to probe into the well-known “doughnut effect” observed in fluorescence images of proteins using the SNOM technique. The topographic and fluorescence SNOM images revealed that the number of proteins at the boundary of the spot were more than at the center in the case of the microarray spot which showed brighter luminescence at the edge than at the center in the confocal image.     

蛋白质—四氯苯醌荷移反应的分光光度研究

本文用分光光度法研究了蛋白质—四氯苯醌荷移反应。通过对影响反应各因素的研究,确立了以四氯苯醌形成荷移络合物的方法测定蛋白质的最佳条件,测定了最大结合数及反应平衡常数。     

1-氨基环丙烷-1-羧酸的合成: 新型植物生长调节剂

本文报道了合成1-氨基环丙烷-1-羧酸的简便而价廉的方法,即乙酰乙酸乙酯法和α-氰乙酸乙酯法;并测定了产品的结构。     

取代硫脲化合物对作物的生长调节作用

取代脲类化合物已广泛用作除草剂、杀虫剂、杀鼠剂。根据结构活性关系,亦对烯丙基、甲基、二芳基取代硫脲衍生物的植物生长调节活性进行了研究。本文合成了3类取代硫脲化合物:R_1NHCSNHR_2,其中:R_1=苯基,α-萘基,叔丁基,R_2=α-苯基乙     

复合蛋白锌在儿科疾患方面的应用

儿童缺锌是较普遍的社会现象，济南泉城微量元素生命研究所研制的复合蛋白锌系采用生物技术转化而得到的一种蛋白复合物，属生物合成的有机微量元素，经急性和长期毒性试验均未发现毒副作用，经检测不含兴奋剂，用于临床后，对纠正偏食厌食，反复呼吸道感染和智力低下等近千例儿童疾患，显示了可喜的临床效果，证明是优质有机补锌剂。     

LC Packing Materials for Pharmaceutical and Biomedical Analysis

 The author has prepared novel liquid chromatography (LC) packing materials for pharmaceutical and biomedical analysis. Those include LC packing materials for direct serum injection assays of drugs and their metabolites, LC packing materials for resolution of enantiomeric drugs, and uniformly sized molecularly imprinted polymers for drugs and their metabolites.     

天花粉蛋白的化学——Ⅴ.粉末状结晶天花粉蛋白的拉曼光谱

本文报道粉末状结晶天花粉蛋白的拉曼光谱。酰胺Ⅰ和酰胺Ⅲ的振动谱带在1680和1250 cm~(-1)。骨架C_α—C—N的振动谱带在965 cm~(-1)。苯丙氨酸残基的特征谱带在1010和1620 cm~(-1)。酪氨酸残基的特征谱带在845和862 cm~(-1)。色氨酸残基的特征谱带在765 cm~(-1)。酰胺Ⅰ和Ⅲ的谱带中,B折叠和无序结构的特征较为明显。表征无序结构的965 cm~(-1)谱带很弱。     

脑神经退行性疾病中的有机化学-朊病毒(疯牛病)中的蛋白物理有机化学和自由基化学

通过关于“普里昂”蛋白病毒疾病的已有临床、医学生理、免疫和化学等方面的现象，讨论了朊病毒当中的部分蛋白氧化损伤和蛋白自由基化学本质。     

The effect of ph and gas composition on the bubble fractionation of proteins

Studies were conducted to establish the effect of the variation of environmental factors on the separation occurring in protein systems, resulting from bubble fractionation in a bioreactor. The measure of separation was selected to be the separation ratio. This is defined to be the ratio of either the top or the middle position concentration in the vessel to the bottom concentration of the vessel. Invertase and α-amylase were the two “model” enzymes considered. It was observed that, under certain conditions, i.e., a combination of the nature of the sparging gas and the medium pH, varying degrees of protein separation were achieved. The pH of the system dramatically influenced the separation. It was found that the best separation occurred at a certain pH, assumed to be at or close to thepI of the protein in question. Furthermore, it was observed that systems sparged with CO₂ exhibited greater separation than systems sparged with air. In fact, in the case of invertase, almost threefold separation was observed at the top port when the solution was sparged with CO₂.     

Growth kinetics and phenolics production inGlycine Max cell suspension cultures

Glycine max was used as a model plant cell suspension culture to establish relationships among growth kinetics, phenolics production, elicitor action, and peroxidase activity. Timing of elictor addition through monitoring of peroxidase provided an excellent means of optimizing yields of phenolics and reduced the time span during which phenolics were formed, negating the need for a secondary production medium. We have also determined that calcium and other cellular effectors like polyamines and organic osmolytes, when used in conjunction with elicitors, enhance phenolics production. Calcium directly enhanced elicitation, whereas polyamines and other osmolytes such as glycerol and proline extended cell viability. The study also demonstrated potential for enhancing secondary metabolite production by a combination of elicitation, cell viability stabilizers, and by addition of nutrients at the time of elicitation.